Rap2a serves as a potential prognostic indicator of renal cell carcinoma and promotes its migration and invasion through up-regulating p-Akt

Abstract Rap2a, a member of the small GTPase superfamily, belongs to Ras superfamily, and its function in cancer progression is still poorly understood. Our previous study indicated that the ectopic expression of Rap2a enhanced the migration and invasion ability of lung cancer cells. However, its ex...

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Autores principales:	Jin-Xia Wu, Wen-Qi Du, Xiu-Cun Wang, Lu-Lu Wei, Fu-Chun Huo, Yao-Jie Pan, Xiao-Jin Wu, Dong-Sheng Pei
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15b014946b634533945d80ccb1662a04
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spelling	oai:doaj.org-article:15b014946b634533945d80ccb1662a042021-12-02T16:08:21ZRap2a serves as a potential prognostic indicator of renal cell carcinoma and promotes its migration and invasion through up-regulating p-Akt10.1038/s41598-017-06162-72045-2322https://doaj.org/article/15b014946b634533945d80ccb1662a042017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06162-7https://doaj.org/toc/2045-2322Abstract Rap2a, a member of the small GTPase superfamily, belongs to Ras superfamily, and its function in cancer progression is still poorly understood. Our previous study indicated that the ectopic expression of Rap2a enhanced the migration and invasion ability of lung cancer cells. However, its expression and molecular mechanism on renal cell carcinoma (RCC) have not been characterized. This study explored the clinical significance and biological function of Rap2a in human RCC. The clinical relevance of Rap2a in RCC was evaluated by immunohistochemical staining using tissue microarray. Our data showed that Rap2a expression was dramatically increased in RCC tissues compared with normal renal tissues. The ectopic expression of Rap2a enhanced the migration and invasive ability of cancer cells. In contrast, downregulation of Rap2a inhibited cell invasion. Rap2a had no effect on the proliferation of RCC cell lines. Meanwhile, Rap2a can regulate the phosphorylation level of Akt in vitro. In vivo studies also showed that Rap2a positively regulated metastasis of renal cancer cells and the expression of p-Akt. These findings indicate that Rap2a promotes RCC metastasis and may serve as a candidate RCC prognostic marker and a potential therapeutic target.Jin-Xia WuWen-Qi DuXiu-Cun WangLu-Lu WeiFu-Chun HuoYao-Jie PanXiao-Jin WuDong-Sheng PeiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jin-Xia Wu Wen-Qi Du Xiu-Cun Wang Lu-Lu Wei Fu-Chun Huo Yao-Jie Pan Xiao-Jin Wu Dong-Sheng Pei Rap2a serves as a potential prognostic indicator of renal cell carcinoma and promotes its migration and invasion through up-regulating p-Akt
description	Abstract Rap2a, a member of the small GTPase superfamily, belongs to Ras superfamily, and its function in cancer progression is still poorly understood. Our previous study indicated that the ectopic expression of Rap2a enhanced the migration and invasion ability of lung cancer cells. However, its expression and molecular mechanism on renal cell carcinoma (RCC) have not been characterized. This study explored the clinical significance and biological function of Rap2a in human RCC. The clinical relevance of Rap2a in RCC was evaluated by immunohistochemical staining using tissue microarray. Our data showed that Rap2a expression was dramatically increased in RCC tissues compared with normal renal tissues. The ectopic expression of Rap2a enhanced the migration and invasive ability of cancer cells. In contrast, downregulation of Rap2a inhibited cell invasion. Rap2a had no effect on the proliferation of RCC cell lines. Meanwhile, Rap2a can regulate the phosphorylation level of Akt in vitro. In vivo studies also showed that Rap2a positively regulated metastasis of renal cancer cells and the expression of p-Akt. These findings indicate that Rap2a promotes RCC metastasis and may serve as a candidate RCC prognostic marker and a potential therapeutic target.
format	article
author	Jin-Xia Wu Wen-Qi Du Xiu-Cun Wang Lu-Lu Wei Fu-Chun Huo Yao-Jie Pan Xiao-Jin Wu Dong-Sheng Pei
author_facet	Jin-Xia Wu Wen-Qi Du Xiu-Cun Wang Lu-Lu Wei Fu-Chun Huo Yao-Jie Pan Xiao-Jin Wu Dong-Sheng Pei
author_sort	Jin-Xia Wu
title	Rap2a serves as a potential prognostic indicator of renal cell carcinoma and promotes its migration and invasion through up-regulating p-Akt
title_short	Rap2a serves as a potential prognostic indicator of renal cell carcinoma and promotes its migration and invasion through up-regulating p-Akt
title_full	Rap2a serves as a potential prognostic indicator of renal cell carcinoma and promotes its migration and invasion through up-regulating p-Akt
title_fullStr	Rap2a serves as a potential prognostic indicator of renal cell carcinoma and promotes its migration and invasion through up-regulating p-Akt
title_full_unstemmed	Rap2a serves as a potential prognostic indicator of renal cell carcinoma and promotes its migration and invasion through up-regulating p-Akt
title_sort	rap2a serves as a potential prognostic indicator of renal cell carcinoma and promotes its migration and invasion through up-regulating p-akt
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/15b014946b634533945d80ccb1662a04
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Rap2a serves as a potential prognostic indicator of renal cell carcinoma and promotes its migration and invasion through up-regulating p-Akt

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