Construction of the Core Collection of Catalpa fargesii f. duclouxii (Huangxinzimu) Based on Molecular Markers and Phenotypic Traits

To promote the conservation and utilization of Catalpa fargesii f. duclouxii (Huangxinzimu) germplasm resources, a total of 252 accessions were used to construct a preliminary core collection according to phenotypic traits and single nucleotide polymorphism (SNP...

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Autores principales:	Huifen Xue, Xiaochi Yu, Pengyue Fu, Bingyang Liu, Shen Zhang, Jie Li, Wenji Zhai, Nan Lu, Xiyang Zhao, Junhui Wang, Wenjun Ma
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Catalpa fargesii</i> f. <i>duclouxii</i> core collection phenotypic traits molecular markers Plant ecology QK900-989
Acceso en línea:	https://doaj.org/article/15b0d59a64144cfb8dc1e2cff81285f4
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spelling	oai:doaj.org-article:15b0d59a64144cfb8dc1e2cff81285f42021-11-25T17:38:11ZConstruction of the Core Collection of <i>Catalpa fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) Based on Molecular Markers and Phenotypic Traits10.3390/f121115181999-4907https://doaj.org/article/15b0d59a64144cfb8dc1e2cff81285f42021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4907/12/11/1518https://doaj.org/toc/1999-4907To promote the conservation and utilization of <i>Catalpa fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) germplasm resources, a total of 252 accessions were used to construct a preliminary core collection according to phenotypic traits and single nucleotide polymorphism (SNP) markers. In this study, 24 phenotypic traits, namely, 9 quantitative traits and 15 qualitative traits, were investigated. The core collection of <i>C. fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) was constructed to remove redundant samples from the collected materials. First, the phenotypic core collection, with a sample proportion of 30, consisting of 24 clones, was constructed according to two genetic distances (Euclidean distance and Mahalanobis), four system clustering methods (the unweighted pair-group average method, Ward’s method, the complete linkage method, and the single linkage method), and three sampling methods (random sampling, deviation sampling, and preferred sampling). The best construction strategies were selected for further comparison. Three core collections (D2C3S3-30, D2C3S3-50, and D2C3S3-70) were constructed according to the optimal construction strategy at three sampling proportions. The core collection D2C3S3-30 with the best parameters was evaluated by using six parameters: the mean difference percentage (<i>MD</i>), variance difference percentage (<i>VD</i>), periodic rate of range (<i>CR</i>), changeable rate of the coefficient of variation (<i>VR</i>), minimum rate of change (<i>CR<sub>MIN</sub></i>), and maximum rate of change (<i>CR<sub>MAX</sub></i>). Three core collections (M-30, M-50, and M-70) were constructed by molecular markers, and the optimal core collection M-30 was selected by using five parameters, namely, <i>H<sub>o</sub></i>, <i>He</i>, <i>PIC</i>, <i>MAF</i>, and <i>loci</i>. The combination of D2C3S3-30 and M-30 was used to construct the final core collection DM-45, 45 samples representing the complete range of phenotypic and genetic variability. In this study, phenotypic traits combined with molecular markers were used to construct core collections to effectively capture the entire range of trait variation, effectively representing the original germplasm and providing a basis for the conservation and utilization of <i>C</i><i>. fargesii</i> f. <i>duclouxii</i> (Huangxinzimu).Huifen XueXiaochi YuPengyue FuBingyang LiuShen ZhangJie LiWenji ZhaiNan LuXiyang ZhaoJunhui WangWenjun MaMDPI AGarticle<i>Catalpa fargesii</i> f. <i>duclouxii</i>core collectionphenotypic traitsmolecular markersPlant ecologyQK900-989ENForests, Vol 12, Iss 1518, p 1518 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Catalpa fargesii</i> f. <i>duclouxii</i> core collection phenotypic traits molecular markers Plant ecology QK900-989
spellingShingle	<i>Catalpa fargesii</i> f. <i>duclouxii</i> core collection phenotypic traits molecular markers Plant ecology QK900-989 Huifen Xue Xiaochi Yu Pengyue Fu Bingyang Liu Shen Zhang Jie Li Wenji Zhai Nan Lu Xiyang Zhao Junhui Wang Wenjun Ma Construction of the Core Collection of <i>Catalpa fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) Based on Molecular Markers and Phenotypic Traits
description	To promote the conservation and utilization of <i>Catalpa fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) germplasm resources, a total of 252 accessions were used to construct a preliminary core collection according to phenotypic traits and single nucleotide polymorphism (SNP) markers. In this study, 24 phenotypic traits, namely, 9 quantitative traits and 15 qualitative traits, were investigated. The core collection of <i>C. fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) was constructed to remove redundant samples from the collected materials. First, the phenotypic core collection, with a sample proportion of 30, consisting of 24 clones, was constructed according to two genetic distances (Euclidean distance and Mahalanobis), four system clustering methods (the unweighted pair-group average method, Ward’s method, the complete linkage method, and the single linkage method), and three sampling methods (random sampling, deviation sampling, and preferred sampling). The best construction strategies were selected for further comparison. Three core collections (D2C3S3-30, D2C3S3-50, and D2C3S3-70) were constructed according to the optimal construction strategy at three sampling proportions. The core collection D2C3S3-30 with the best parameters was evaluated by using six parameters: the mean difference percentage (<i>MD</i>), variance difference percentage (<i>VD</i>), periodic rate of range (<i>CR</i>), changeable rate of the coefficient of variation (<i>VR</i>), minimum rate of change (<i>CR<sub>MIN</sub></i>), and maximum rate of change (<i>CR<sub>MAX</sub></i>). Three core collections (M-30, M-50, and M-70) were constructed by molecular markers, and the optimal core collection M-30 was selected by using five parameters, namely, <i>H<sub>o</sub></i>, <i>He</i>, <i>PIC</i>, <i>MAF</i>, and <i>loci</i>. The combination of D2C3S3-30 and M-30 was used to construct the final core collection DM-45, 45 samples representing the complete range of phenotypic and genetic variability. In this study, phenotypic traits combined with molecular markers were used to construct core collections to effectively capture the entire range of trait variation, effectively representing the original germplasm and providing a basis for the conservation and utilization of <i>C</i><i>. fargesii</i> f. <i>duclouxii</i> (Huangxinzimu).
format	article
author	Huifen Xue Xiaochi Yu Pengyue Fu Bingyang Liu Shen Zhang Jie Li Wenji Zhai Nan Lu Xiyang Zhao Junhui Wang Wenjun Ma
author_facet	Huifen Xue Xiaochi Yu Pengyue Fu Bingyang Liu Shen Zhang Jie Li Wenji Zhai Nan Lu Xiyang Zhao Junhui Wang Wenjun Ma
author_sort	Huifen Xue
title	Construction of the Core Collection of <i>Catalpa fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) Based on Molecular Markers and Phenotypic Traits
title_short	Construction of the Core Collection of <i>Catalpa fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) Based on Molecular Markers and Phenotypic Traits
title_full	Construction of the Core Collection of <i>Catalpa fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) Based on Molecular Markers and Phenotypic Traits
title_fullStr	Construction of the Core Collection of <i>Catalpa fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) Based on Molecular Markers and Phenotypic Traits
title_full_unstemmed	Construction of the Core Collection of <i>Catalpa fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) Based on Molecular Markers and Phenotypic Traits
title_sort	construction of the core collection of <i>catalpa fargesii</i> f. <i>duclouxii</i> (huangxinzimu) based on molecular markers and phenotypic traits
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/15b0d59a64144cfb8dc1e2cff81285f4
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Construction of the Core Collection of <i>Catalpa fargesii</i> f. <i>duclouxii</i> (Huangxinzimu) Based on Molecular Markers and Phenotypic Traits

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