Ultrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal

Abstract A novel sandwich-assay electrochemical immunosensor for simultaneous determination of autophagic biomarkers was introduced for the first time, the gold-reduced grapheme oxide nanocomposite (Au/r-GO) set as a good conductive platform with super high specific area, and provided more binding s...

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Autores principales:	Guannan Wang, Yankun Li, Jinlei Liu, Yajing Yuan, Zhaoliang Shen, Xifan Mei
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15b1ff8f2f9a4374a7028302a343194e
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spelling	oai:doaj.org-article:15b1ff8f2f9a4374a7028302a343194e2021-12-02T16:06:55ZUltrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal10.1038/s41598-017-02766-12045-2322https://doaj.org/article/15b1ff8f2f9a4374a7028302a343194e2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02766-1https://doaj.org/toc/2045-2322Abstract A novel sandwich-assay electrochemical immunosensor for simultaneous determination of autophagic biomarkers was introduced for the first time, the gold-reduced grapheme oxide nanocomposite (Au/r-GO) set as a good conductive platform with super high specific area, and provided more binding sites for the both antibodies of Beclin-1 and LC3B-II. While Au nanocages (AuNCs) served as good conductive platform to encapsulate a large amount of redox probe and secondary antibodies for signal amplification, due to the abundant reactive oxygen functional groups on its surface. Through differential pulse voltammetry (DPV) measurements, two separate signals can be detected directly in a single run, which represent the existence of Belin-1 and LC3B-II. Under optimized conditions, the electrochemical immunosensor exhibited good sensitivity and selectivity for the simultaneous determination of Beclin-1 and LC3B-II with linear ranges of 0.1–100 ng/mL. The detection limit for Beclin-1 and LC3B-II is 0.02 and 0.03 ng/mL respectively. This method was also applied for the analysis of Beclin-1 and LC3B-II levels in experimental cellular protein lysates, and the results were in good agreement with those of enzyme linked immunosorbent assay. This approach gives a promising simple, sensitive and quantitative strategy for the detection of autophagyGuannan WangYankun LiJinlei LiuYajing YuanZhaoliang ShenXifan MeiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guannan Wang Yankun Li Jinlei Liu Yajing Yuan Zhaoliang Shen Xifan Mei Ultrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal
description	Abstract A novel sandwich-assay electrochemical immunosensor for simultaneous determination of autophagic biomarkers was introduced for the first time, the gold-reduced grapheme oxide nanocomposite (Au/r-GO) set as a good conductive platform with super high specific area, and provided more binding sites for the both antibodies of Beclin-1 and LC3B-II. While Au nanocages (AuNCs) served as good conductive platform to encapsulate a large amount of redox probe and secondary antibodies for signal amplification, due to the abundant reactive oxygen functional groups on its surface. Through differential pulse voltammetry (DPV) measurements, two separate signals can be detected directly in a single run, which represent the existence of Belin-1 and LC3B-II. Under optimized conditions, the electrochemical immunosensor exhibited good sensitivity and selectivity for the simultaneous determination of Beclin-1 and LC3B-II with linear ranges of 0.1–100 ng/mL. The detection limit for Beclin-1 and LC3B-II is 0.02 and 0.03 ng/mL respectively. This method was also applied for the analysis of Beclin-1 and LC3B-II levels in experimental cellular protein lysates, and the results were in good agreement with those of enzyme linked immunosorbent assay. This approach gives a promising simple, sensitive and quantitative strategy for the detection of autophagy
format	article
author	Guannan Wang Yankun Li Jinlei Liu Yajing Yuan Zhaoliang Shen Xifan Mei
author_facet	Guannan Wang Yankun Li Jinlei Liu Yajing Yuan Zhaoliang Shen Xifan Mei
author_sort	Guannan Wang
title	Ultrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal
title_short	Ultrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal
title_full	Ultrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal
title_fullStr	Ultrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal
title_full_unstemmed	Ultrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal
title_sort	ultrasensitive multiplexed immunoassay of autophagic biomarkers based on au/rgo and au nanocages amplifying electrochemcial signal
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/15b1ff8f2f9a4374a7028302a343194e
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_version_	1718384762419150848

Ultrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal

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