Transcriptome Analysis in the Head Kidney of Rainbow Trout (Oncorhynchus mykiss) Immunized with a Combined Vaccine of Formalin-Inactivated Aeromonas salmonicida and Vibrio anguillarum

This study aimed to identify the molecular mechanisms regulated by a combined vaccine against Aeromonas salmonicida and Vibrio anguillarum (O1 serotype). These bacteria cause furunculosis and vibriosis, respectively, and are associated with a high mortality in r...

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Autores principales:	Jongwon Lim, Suhee Hong
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	vaccine <i>A. salmonicida</i> <i>V. anguillarum</i> transcriptome immune Medicine R
Acceso en línea:	https://doaj.org/article/15bd88e00a7249c0b704ef626cf7d6fc
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spelling	oai:doaj.org-article:15bd88e00a7249c0b704ef626cf7d6fc2021-11-25T19:10:20ZTranscriptome Analysis in the Head Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Immunized with a Combined Vaccine of Formalin-Inactivated <i>Aeromonas salmonicida</i> and <i>Vibrio anguillarum</i>10.3390/vaccines91112342076-393Xhttps://doaj.org/article/15bd88e00a7249c0b704ef626cf7d6fc2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1234https://doaj.org/toc/2076-393XThis study aimed to identify the molecular mechanisms regulated by a combined vaccine against <i>Aeromonas salmonicida</i> and <i>Vibrio anguillarum</i> (O1 serotype). These bacteria cause furunculosis and vibriosis, respectively, and are associated with a high mortality in rainbow trout in Korea. The vaccine upregulated gene expression of TCRα, T-bet, sIgM, and mIgM, markers of an activated adaptive immune response. On days 1, 3, and 5, transcriptome analysis revealed 862 (430 up- and 432 downregulated), 492 (204 up- and 288 downregulated), and 741 (270 up- and 471 downregulated) differentially expressed genes (DEGs), respectively. Gene ontology (GO) enrichment analysis identified 377 (108 MF, 132 CC, 137 BP), 302 (60 MF, 180 CC, 62 BP), and 314 (115 MF, 129 CC, 70 BP) GOs at days 1, 3, and 5, respectively. Kyoto Encyclopedia of Genetic and Genomic enrichment analysis identified eight immune system-related pathways like cytokine-cytokine receptor interaction, NF-kappaB signaling pathway, TNF signaling pathway, NOD-like receptor signaling pathway, cytosolic DNA sensing pathway, cell adhesion molecule, complement and coagulation cascade, and antigen processing and presentation. In the analysis of the protein–protein interaction of immune-related DEGs, a total of 59, 21, and 21 interactional relationships were identified at days 1, 3, and 5, respectively, with TNF having the highest centrality at all three time points.Jongwon LimSuhee HongMDPI AGarticlevaccine<i>A. salmonicida</i><i>V. anguillarum</i>transcriptomeimmuneMedicineRENVaccines, Vol 9, Iss 1234, p 1234 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vaccine <i>A. salmonicida</i> <i>V. anguillarum</i> transcriptome immune Medicine R
spellingShingle	vaccine <i>A. salmonicida</i> <i>V. anguillarum</i> transcriptome immune Medicine R Jongwon Lim Suhee Hong Transcriptome Analysis in the Head Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Immunized with a Combined Vaccine of Formalin-Inactivated <i>Aeromonas salmonicida</i> and <i>Vibrio anguillarum</i>
description	This study aimed to identify the molecular mechanisms regulated by a combined vaccine against <i>Aeromonas salmonicida</i> and <i>Vibrio anguillarum</i> (O1 serotype). These bacteria cause furunculosis and vibriosis, respectively, and are associated with a high mortality in rainbow trout in Korea. The vaccine upregulated gene expression of TCRα, T-bet, sIgM, and mIgM, markers of an activated adaptive immune response. On days 1, 3, and 5, transcriptome analysis revealed 862 (430 up- and 432 downregulated), 492 (204 up- and 288 downregulated), and 741 (270 up- and 471 downregulated) differentially expressed genes (DEGs), respectively. Gene ontology (GO) enrichment analysis identified 377 (108 MF, 132 CC, 137 BP), 302 (60 MF, 180 CC, 62 BP), and 314 (115 MF, 129 CC, 70 BP) GOs at days 1, 3, and 5, respectively. Kyoto Encyclopedia of Genetic and Genomic enrichment analysis identified eight immune system-related pathways like cytokine-cytokine receptor interaction, NF-kappaB signaling pathway, TNF signaling pathway, NOD-like receptor signaling pathway, cytosolic DNA sensing pathway, cell adhesion molecule, complement and coagulation cascade, and antigen processing and presentation. In the analysis of the protein–protein interaction of immune-related DEGs, a total of 59, 21, and 21 interactional relationships were identified at days 1, 3, and 5, respectively, with TNF having the highest centrality at all three time points.
format	article
author	Jongwon Lim Suhee Hong
author_facet	Jongwon Lim Suhee Hong
author_sort	Jongwon Lim
title	Transcriptome Analysis in the Head Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Immunized with a Combined Vaccine of Formalin-Inactivated <i>Aeromonas salmonicida</i> and <i>Vibrio anguillarum</i>
title_short	Transcriptome Analysis in the Head Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Immunized with a Combined Vaccine of Formalin-Inactivated <i>Aeromonas salmonicida</i> and <i>Vibrio anguillarum</i>
title_full	Transcriptome Analysis in the Head Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Immunized with a Combined Vaccine of Formalin-Inactivated <i>Aeromonas salmonicida</i> and <i>Vibrio anguillarum</i>
title_fullStr	Transcriptome Analysis in the Head Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Immunized with a Combined Vaccine of Formalin-Inactivated <i>Aeromonas salmonicida</i> and <i>Vibrio anguillarum</i>
title_full_unstemmed	Transcriptome Analysis in the Head Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Immunized with a Combined Vaccine of Formalin-Inactivated <i>Aeromonas salmonicida</i> and <i>Vibrio anguillarum</i>
title_sort	transcriptome analysis in the head kidney of rainbow trout (<i>oncorhynchus mykiss</i>) immunized with a combined vaccine of formalin-inactivated <i>aeromonas salmonicida</i> and <i>vibrio anguillarum</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/15bd88e00a7249c0b704ef626cf7d6fc
work_keys_str_mv	AT jongwonlim transcriptomeanalysisintheheadkidneyofrainbowtroutioncorhynchusmykissiimmunizedwithacombinedvaccineofformalininactivatediaeromonassalmonicidaiandivibrioanguillarumi AT suheehong transcriptomeanalysisintheheadkidneyofrainbowtroutioncorhynchusmykissiimmunizedwithacombinedvaccineofformalininactivatediaeromonassalmonicidaiandivibrioanguillarumi
_version_	1718410199604133888

Transcriptome Analysis in the Head Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Immunized with a Combined Vaccine of Formalin-Inactivated <i>Aeromonas salmonicida</i> and <i>Vibrio anguillarum</i>

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