RSF governs silent chromatin formation via histone H2Av replacement.

Human remodeling and spacing factor (RSF) consists of a heterodimer of Rsf-1 and hSNF2H, a counterpart of Drosophila ISWI. RSF possesses not only chromatin remodeling activity but also chromatin assembly activity in vitro. While no other single factor can execute the same activities as RSF, the biol...

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Autores principales:	Kazuma Hanai, Hirofumi Furuhashi, Takashi Yamamoto, Koji Akasaka, Susumu Hirose
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/15bf9608f0b142db91a61f21993b4184
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spelling	oai:doaj.org-article:15bf9608f0b142db91a61f21993b41842021-11-25T05:52:15ZRSF governs silent chromatin formation via histone H2Av replacement.1553-73901553-740410.1371/journal.pgen.1000011https://doaj.org/article/15bf9608f0b142db91a61f21993b41842008-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18454204/pdf/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Human remodeling and spacing factor (RSF) consists of a heterodimer of Rsf-1 and hSNF2H, a counterpart of Drosophila ISWI. RSF possesses not only chromatin remodeling activity but also chromatin assembly activity in vitro. While no other single factor can execute the same activities as RSF, the biological significance of RSF remained unknown. To investigate the in vivo function of RSF, we generated a mutant allele of Drosophila Rsf-1 (dRsf-1). The dRsf-1 mutant behaved as a dominant suppressor of position effect variegation. In dRsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin. Furthermore, using both genetic and biochemical approaches, we demonstrate that dRsf-1 interacts with H2Av and the H2Av-exchanging machinery Tip60 complex. These results suggest that RSF contributes to histone H2Av replacement in the pathway of silent chromatin formation.Kazuma HanaiHirofumi FuruhashiTakashi YamamotoKoji AkasakaSusumu HirosePublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 4, Iss 2, p e1000011 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Kazuma Hanai Hirofumi Furuhashi Takashi Yamamoto Koji Akasaka Susumu Hirose RSF governs silent chromatin formation via histone H2Av replacement.
description	Human remodeling and spacing factor (RSF) consists of a heterodimer of Rsf-1 and hSNF2H, a counterpart of Drosophila ISWI. RSF possesses not only chromatin remodeling activity but also chromatin assembly activity in vitro. While no other single factor can execute the same activities as RSF, the biological significance of RSF remained unknown. To investigate the in vivo function of RSF, we generated a mutant allele of Drosophila Rsf-1 (dRsf-1). The dRsf-1 mutant behaved as a dominant suppressor of position effect variegation. In dRsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin. Furthermore, using both genetic and biochemical approaches, we demonstrate that dRsf-1 interacts with H2Av and the H2Av-exchanging machinery Tip60 complex. These results suggest that RSF contributes to histone H2Av replacement in the pathway of silent chromatin formation.
format	article
author	Kazuma Hanai Hirofumi Furuhashi Takashi Yamamoto Koji Akasaka Susumu Hirose
author_facet	Kazuma Hanai Hirofumi Furuhashi Takashi Yamamoto Koji Akasaka Susumu Hirose
author_sort	Kazuma Hanai
title	RSF governs silent chromatin formation via histone H2Av replacement.
title_short	RSF governs silent chromatin formation via histone H2Av replacement.
title_full	RSF governs silent chromatin formation via histone H2Av replacement.
title_fullStr	RSF governs silent chromatin formation via histone H2Av replacement.
title_full_unstemmed	RSF governs silent chromatin formation via histone H2Av replacement.
title_sort	rsf governs silent chromatin formation via histone h2av replacement.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/15bf9608f0b142db91a61f21993b4184
work_keys_str_mv	AT kazumahanai rsfgovernssilentchromatinformationviahistoneh2avreplacement AT hirofumifuruhashi rsfgovernssilentchromatinformationviahistoneh2avreplacement AT takashiyamamoto rsfgovernssilentchromatinformationviahistoneh2avreplacement AT kojiakasaka rsfgovernssilentchromatinformationviahistoneh2avreplacement AT susumuhirose rsfgovernssilentchromatinformationviahistoneh2avreplacement
_version_	1718414383686615040

RSF governs silent chromatin formation via histone H2Av replacement.

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