Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis

Abstract Background GenoLab M is a recently established next-generation sequencing platform from GeneMind Biosciences. Presently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Here, we present the first report to compare the transcriptome...

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Autores principales:	Yongfeng Liu, Ran Han, Letian Zhou, Mingjie Luo, Lidong Zeng, Xiaochao Zhao, Yukun Ma, Zhiliang Zhou, Lei Sun
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	GenoLab M NovaSeq 6000 Transcriptome LncRNA Compatibility Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/15c526273cd648518d63065256f691dd
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spelling	oai:doaj.org-article:15c526273cd648518d63065256f691dd2021-11-21T12:26:38ZComparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis10.1186/s12864-021-08150-81471-2164https://doaj.org/article/15c526273cd648518d63065256f691dd2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08150-8https://doaj.org/toc/1471-2164Abstract Background GenoLab M is a recently established next-generation sequencing platform from GeneMind Biosciences. Presently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Here, we present the first report to compare the transcriptome and LncRNA sequencing data of the GenoLab M sequencer to NovaSeq 6000 platform in various types of analysis. Results We tested 16 libraries in three species using various library kits from different companies. We compared the data quality, genes expression, alternatively spliced (AS) events, single nucleotide polymorphism (SNP), and insertions–deletions (InDel) between two sequencing platforms. The data suggested that platforms have comparable sensitivity and accuracy in terms of quantification of gene expression levels with technical compatibility. Conclusions Genolab M is a promising next-generation sequencing platform for transcriptomics and LncRNA studies with high performance at low costs.Yongfeng LiuRan HanLetian ZhouMingjie LuoLidong ZengXiaochao ZhaoYukun MaZhiliang ZhouLei SunBMCarticleGenoLab MNovaSeq 6000TranscriptomeLncRNACompatibilityBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	GenoLab M NovaSeq 6000 Transcriptome LncRNA Compatibility Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	GenoLab M NovaSeq 6000 Transcriptome LncRNA Compatibility Biotechnology TP248.13-248.65 Genetics QH426-470 Yongfeng Liu Ran Han Letian Zhou Mingjie Luo Lidong Zeng Xiaochao Zhao Yukun Ma Zhiliang Zhou Lei Sun Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis
description	Abstract Background GenoLab M is a recently established next-generation sequencing platform from GeneMind Biosciences. Presently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Here, we present the first report to compare the transcriptome and LncRNA sequencing data of the GenoLab M sequencer to NovaSeq 6000 platform in various types of analysis. Results We tested 16 libraries in three species using various library kits from different companies. We compared the data quality, genes expression, alternatively spliced (AS) events, single nucleotide polymorphism (SNP), and insertions–deletions (InDel) between two sequencing platforms. The data suggested that platforms have comparable sensitivity and accuracy in terms of quantification of gene expression levels with technical compatibility. Conclusions Genolab M is a promising next-generation sequencing platform for transcriptomics and LncRNA studies with high performance at low costs.
format	article
author	Yongfeng Liu Ran Han Letian Zhou Mingjie Luo Lidong Zeng Xiaochao Zhao Yukun Ma Zhiliang Zhou Lei Sun
author_facet	Yongfeng Liu Ran Han Letian Zhou Mingjie Luo Lidong Zeng Xiaochao Zhao Yukun Ma Zhiliang Zhou Lei Sun
author_sort	Yongfeng Liu
title	Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis
title_short	Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis
title_full	Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis
title_fullStr	Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis
title_full_unstemmed	Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis
title_sort	comparative performance of the genolab m and novaseq 6000 sequencing platforms for transcriptome and lncrna analysis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/15c526273cd648518d63065256f691dd
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Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis

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