Rapid adaptation to microgravity in mammalian macrophage cells
Abstract Despite the observed severe effects of microgravity on mammalian cells, many astronauts have completed long term stays in space without suffering from severe health problems. This raises questions about the cellular capacity for adaptation to a new gravitational environment. The Internation...
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Nature Portfolio
2017
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oai:doaj.org-article:15cba96c636e43309fa596cd593879c62021-12-02T11:53:03ZRapid adaptation to microgravity in mammalian macrophage cells10.1038/s41598-017-00119-62045-2322https://doaj.org/article/15cba96c636e43309fa596cd593879c62017-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00119-6https://doaj.org/toc/2045-2322Abstract Despite the observed severe effects of microgravity on mammalian cells, many astronauts have completed long term stays in space without suffering from severe health problems. This raises questions about the cellular capacity for adaptation to a new gravitational environment. The International Space Station (ISS) experiment TRIPLE LUX A, performed in the BIOLAB laboratory of the ISS COLUMBUS module, allowed for the first time the direct measurement of a cellular function in real time and on orbit. We measured the oxidative burst reaction in mammalian macrophages (NR8383 rat alveolar macrophages) exposed to a centrifuge regime of internal 0 g and 1 g controls and step-wise increase or decrease of the gravitational force in four independent experiments. Surprisingly, we found that these macrophages adapted to microgravity in an ultra-fast manner within seconds, after an immediate inhibitory effect on the oxidative burst reaction. For the first time, we provided direct evidence of cellular sensitivity to gravity, through real-time on orbit measurements and by using an experimental system, in which all factors except gravity were constant. The surprisingly ultra-fast adaptation to microgravity indicates that mammalian macrophages are equipped with a highly efficient adaptation potential to a low gravity environment. This opens new avenues for the exploration of adaptation of mammalian cells to gravitational changes.Cora S. ThielDiane de ZélicourtSvantje TauberAstrid AdrianMarkus FranzDana M. SimmetKathrin SchoppmannSwantje HauschildSonja KrammerMiriam ChristenGesine BradacsKatrin PaulsenSusanne A. WolfMarkus BraunJason HattonVartan KurtcuogluStefanie FrankeSamuel TannerSamantha CristoforettiBeate SickBertold HockOliver UllrichNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
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Medicine R Science Q Cora S. Thiel Diane de Zélicourt Svantje Tauber Astrid Adrian Markus Franz Dana M. Simmet Kathrin Schoppmann Swantje Hauschild Sonja Krammer Miriam Christen Gesine Bradacs Katrin Paulsen Susanne A. Wolf Markus Braun Jason Hatton Vartan Kurtcuoglu Stefanie Franke Samuel Tanner Samantha Cristoforetti Beate Sick Bertold Hock Oliver Ullrich Rapid adaptation to microgravity in mammalian macrophage cells |
| description |
Abstract Despite the observed severe effects of microgravity on mammalian cells, many astronauts have completed long term stays in space without suffering from severe health problems. This raises questions about the cellular capacity for adaptation to a new gravitational environment. The International Space Station (ISS) experiment TRIPLE LUX A, performed in the BIOLAB laboratory of the ISS COLUMBUS module, allowed for the first time the direct measurement of a cellular function in real time and on orbit. We measured the oxidative burst reaction in mammalian macrophages (NR8383 rat alveolar macrophages) exposed to a centrifuge regime of internal 0 g and 1 g controls and step-wise increase or decrease of the gravitational force in four independent experiments. Surprisingly, we found that these macrophages adapted to microgravity in an ultra-fast manner within seconds, after an immediate inhibitory effect on the oxidative burst reaction. For the first time, we provided direct evidence of cellular sensitivity to gravity, through real-time on orbit measurements and by using an experimental system, in which all factors except gravity were constant. The surprisingly ultra-fast adaptation to microgravity indicates that mammalian macrophages are equipped with a highly efficient adaptation potential to a low gravity environment. This opens new avenues for the exploration of adaptation of mammalian cells to gravitational changes. |
| format |
article |
| author |
Cora S. Thiel Diane de Zélicourt Svantje Tauber Astrid Adrian Markus Franz Dana M. Simmet Kathrin Schoppmann Swantje Hauschild Sonja Krammer Miriam Christen Gesine Bradacs Katrin Paulsen Susanne A. Wolf Markus Braun Jason Hatton Vartan Kurtcuoglu Stefanie Franke Samuel Tanner Samantha Cristoforetti Beate Sick Bertold Hock Oliver Ullrich |
| author_facet |
Cora S. Thiel Diane de Zélicourt Svantje Tauber Astrid Adrian Markus Franz Dana M. Simmet Kathrin Schoppmann Swantje Hauschild Sonja Krammer Miriam Christen Gesine Bradacs Katrin Paulsen Susanne A. Wolf Markus Braun Jason Hatton Vartan Kurtcuoglu Stefanie Franke Samuel Tanner Samantha Cristoforetti Beate Sick Bertold Hock Oliver Ullrich |
| author_sort |
Cora S. Thiel |
| title |
Rapid adaptation to microgravity in mammalian macrophage cells |
| title_short |
Rapid adaptation to microgravity in mammalian macrophage cells |
| title_full |
Rapid adaptation to microgravity in mammalian macrophage cells |
| title_fullStr |
Rapid adaptation to microgravity in mammalian macrophage cells |
| title_full_unstemmed |
Rapid adaptation to microgravity in mammalian macrophage cells |
| title_sort |
rapid adaptation to microgravity in mammalian macrophage cells |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/15cba96c636e43309fa596cd593879c6 |
| work_keys_str_mv |
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