Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities

Abstract Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE...

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Autores principales:	Dvison de Melo Pacífico, Cecília Leite Costa, Hercules Moura, John R. Barr, Guilherme Augusto Maia, Vilmar Benetti Filho, Renato Simões Moreira, Glauber Wagner, Regina Maria Cavalcanti Pilotto Domingues, Carlos Quesada-Gómez, Eliane de Oliveira Ferreira, Gerly Anne de Castro Brito
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15d044fdb0654e53b00cc62d46238c97
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spelling	oai:doaj.org-article:15d044fdb0654e53b00cc62d46238c972021-12-02T17:12:18ZExoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities10.1038/s41598-021-92684-02045-2322https://doaj.org/article/15d044fdb0654e53b00cc62d46238c972021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92684-0https://doaj.org/toc/2045-2322Abstract Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liquid chromatography-tandem mass spectrometry. Genomic analysis revealed that these isolates shared most of the gene composition. Only 83 and 290 NAP1/027 genes were considered singletons in ICC-45 and NAP1/027, respectively. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only five proteins were exclusive to the ICC-45 strain. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC-45 strain; 14 proteins, including mature S-layer protein, were present in higher proportions in LIBA5756. Data are available via ProteomeXchange with identifier PXD026218. These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains.Dvison de Melo PacíficoCecília Leite CostaHercules MouraJohn R. BarrGuilherme Augusto MaiaVilmar Benetti FilhoRenato Simões MoreiraGlauber WagnerRegina Maria Cavalcanti Pilotto DominguesCarlos Quesada-GómezEliane de Oliveira FerreiraGerly Anne de Castro BritoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dvison de Melo Pacífico Cecília Leite Costa Hercules Moura John R. Barr Guilherme Augusto Maia Vilmar Benetti Filho Renato Simões Moreira Glauber Wagner Regina Maria Cavalcanti Pilotto Domingues Carlos Quesada-Gómez Eliane de Oliveira Ferreira Gerly Anne de Castro Brito Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
description	Abstract Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liquid chromatography-tandem mass spectrometry. Genomic analysis revealed that these isolates shared most of the gene composition. Only 83 and 290 NAP1/027 genes were considered singletons in ICC-45 and NAP1/027, respectively. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only five proteins were exclusive to the ICC-45 strain. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC-45 strain; 14 proteins, including mature S-layer protein, were present in higher proportions in LIBA5756. Data are available via ProteomeXchange with identifier PXD026218. These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains.
format	article
author	Dvison de Melo Pacífico Cecília Leite Costa Hercules Moura John R. Barr Guilherme Augusto Maia Vilmar Benetti Filho Renato Simões Moreira Glauber Wagner Regina Maria Cavalcanti Pilotto Domingues Carlos Quesada-Gómez Eliane de Oliveira Ferreira Gerly Anne de Castro Brito
author_facet	Dvison de Melo Pacífico Cecília Leite Costa Hercules Moura John R. Barr Guilherme Augusto Maia Vilmar Benetti Filho Renato Simões Moreira Glauber Wagner Regina Maria Cavalcanti Pilotto Domingues Carlos Quesada-Gómez Eliane de Oliveira Ferreira Gerly Anne de Castro Brito
author_sort	Dvison de Melo Pacífico
title	Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title_short	Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title_full	Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title_fullStr	Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title_full_unstemmed	Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title_sort	exoproteomic analysis of two mlst clade 2 strains of clostridioides difficile from latin america reveal close similarities
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/15d044fdb0654e53b00cc62d46238c97
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Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities

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