Open field release of genetically engineered sterile male Aedes aegypti in Malaysia.
<h4>Background</h4>Dengue is the most important mosquito-borne viral disease. In the absence of specific drugs or vaccines, control focuses on suppressing the principal mosquito vector, Aedes aegypti, yet current methods have not proven adequate to control the disease. New methods are th...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/15d1f1254ff84a4eb81b3ceaad8e50d0 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:15d1f1254ff84a4eb81b3ceaad8e50d0 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:15d1f1254ff84a4eb81b3ceaad8e50d02021-11-18T07:07:27ZOpen field release of genetically engineered sterile male Aedes aegypti in Malaysia.1932-620310.1371/journal.pone.0042771https://doaj.org/article/15d1f1254ff84a4eb81b3ceaad8e50d02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22970102/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Dengue is the most important mosquito-borne viral disease. In the absence of specific drugs or vaccines, control focuses on suppressing the principal mosquito vector, Aedes aegypti, yet current methods have not proven adequate to control the disease. New methods are therefore urgently needed, for example genetics-based sterile-male-release methods. However, this requires that lab-reared, modified mosquitoes be able to survive and disperse adequately in the field.<h4>Methodology/principal findings</h4>Adult male mosquitoes were released into an uninhabited forested area of Pahang, Malaysia. Their survival and dispersal was assessed by use of a network of traps. Two strains were used, an engineered 'genetically sterile' (OX513A) and a wild-type laboratory strain, to give both absolute and relative data about the performance of the modified mosquitoes. The two strains had similar maximum dispersal distances (220 m), but mean distance travelled of the OX513A strain was lower (52 vs. 100 m). Life expectancy was similar (2.0 vs. 2.2 days). Recapture rates were high for both strains, possibly because of the uninhabited nature of the site.<h4>Conclusions/significance</h4>After extensive contained studies and regulatory scrutiny, a field release of engineered mosquitoes was safely and successfully conducted in Malaysia. The engineered strain showed similar field longevity to an unmodified counterpart, though in this setting dispersal was reduced relative to the unmodified strain. These data are encouraging for the future testing and implementation of genetic control strategies and will help guide future field use of this and other engineered strains.Renaud LacroixAndrew R McKemeyNorzahira RaduanLim Kwee WeeWong Hong MingTeoh Guat NeySiti Rahidah A ASawaluddin SalmanSelvi SubramaniamOreenaiza NordinNorhaida Hanum A TChandru AngamuthuSuria Marlina MansorRosemary S LeesNeil NaishSarah ScaifePam GrayGeneviève LabbéCamilla BeechDerric NimmoLuke AlpheySeshadri S VasanLee Han LimNazni Wasi AShahnaz MuradPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42771 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Renaud Lacroix Andrew R McKemey Norzahira Raduan Lim Kwee Wee Wong Hong Ming Teoh Guat Ney Siti Rahidah A A Sawaluddin Salman Selvi Subramaniam Oreenaiza Nordin Norhaida Hanum A T Chandru Angamuthu Suria Marlina Mansor Rosemary S Lees Neil Naish Sarah Scaife Pam Gray Geneviève Labbé Camilla Beech Derric Nimmo Luke Alphey Seshadri S Vasan Lee Han Lim Nazni Wasi A Shahnaz Murad Open field release of genetically engineered sterile male Aedes aegypti in Malaysia. |
| description |
<h4>Background</h4>Dengue is the most important mosquito-borne viral disease. In the absence of specific drugs or vaccines, control focuses on suppressing the principal mosquito vector, Aedes aegypti, yet current methods have not proven adequate to control the disease. New methods are therefore urgently needed, for example genetics-based sterile-male-release methods. However, this requires that lab-reared, modified mosquitoes be able to survive and disperse adequately in the field.<h4>Methodology/principal findings</h4>Adult male mosquitoes were released into an uninhabited forested area of Pahang, Malaysia. Their survival and dispersal was assessed by use of a network of traps. Two strains were used, an engineered 'genetically sterile' (OX513A) and a wild-type laboratory strain, to give both absolute and relative data about the performance of the modified mosquitoes. The two strains had similar maximum dispersal distances (220 m), but mean distance travelled of the OX513A strain was lower (52 vs. 100 m). Life expectancy was similar (2.0 vs. 2.2 days). Recapture rates were high for both strains, possibly because of the uninhabited nature of the site.<h4>Conclusions/significance</h4>After extensive contained studies and regulatory scrutiny, a field release of engineered mosquitoes was safely and successfully conducted in Malaysia. The engineered strain showed similar field longevity to an unmodified counterpart, though in this setting dispersal was reduced relative to the unmodified strain. These data are encouraging for the future testing and implementation of genetic control strategies and will help guide future field use of this and other engineered strains. |
| format |
article |
| author |
Renaud Lacroix Andrew R McKemey Norzahira Raduan Lim Kwee Wee Wong Hong Ming Teoh Guat Ney Siti Rahidah A A Sawaluddin Salman Selvi Subramaniam Oreenaiza Nordin Norhaida Hanum A T Chandru Angamuthu Suria Marlina Mansor Rosemary S Lees Neil Naish Sarah Scaife Pam Gray Geneviève Labbé Camilla Beech Derric Nimmo Luke Alphey Seshadri S Vasan Lee Han Lim Nazni Wasi A Shahnaz Murad |
| author_facet |
Renaud Lacroix Andrew R McKemey Norzahira Raduan Lim Kwee Wee Wong Hong Ming Teoh Guat Ney Siti Rahidah A A Sawaluddin Salman Selvi Subramaniam Oreenaiza Nordin Norhaida Hanum A T Chandru Angamuthu Suria Marlina Mansor Rosemary S Lees Neil Naish Sarah Scaife Pam Gray Geneviève Labbé Camilla Beech Derric Nimmo Luke Alphey Seshadri S Vasan Lee Han Lim Nazni Wasi A Shahnaz Murad |
| author_sort |
Renaud Lacroix |
| title |
Open field release of genetically engineered sterile male Aedes aegypti in Malaysia. |
| title_short |
Open field release of genetically engineered sterile male Aedes aegypti in Malaysia. |
| title_full |
Open field release of genetically engineered sterile male Aedes aegypti in Malaysia. |
| title_fullStr |
Open field release of genetically engineered sterile male Aedes aegypti in Malaysia. |
| title_full_unstemmed |
Open field release of genetically engineered sterile male Aedes aegypti in Malaysia. |
| title_sort |
open field release of genetically engineered sterile male aedes aegypti in malaysia. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/15d1f1254ff84a4eb81b3ceaad8e50d0 |
| work_keys_str_mv |
AT renaudlacroix openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT andrewrmckemey openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT norzahiraraduan openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT limkweewee openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT wonghongming openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT teohguatney openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT sitirahidahaa openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT sawaluddinsalman openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT selvisubramaniam openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT oreenaizanordin openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT norhaidahanumat openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT chandruangamuthu openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT suriamarlinamansor openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT rosemaryslees openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT neilnaish openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT sarahscaife openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT pamgray openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT genevievelabbe openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT camillabeech openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT derricnimmo openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT lukealphey openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT seshadrisvasan openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT leehanlim openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT nazniwasia openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia AT shahnazmurad openfieldreleaseofgeneticallyengineeredsterilemaleaedesaegyptiinmalaysia |
| _version_ |
1718423900303392768 |