Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage

Abstract Understanding the molecular and biochemical basis of egg development is a central topic in mosquito reproductive biology. Lipids are a major source of energy and building blocks for the developing ovarian follicles. Ultra-High Resolution Mass Spectrometry (UHRMS) combined with in vivo metab...

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Autores principales:	Lilian Valadares Tose, Chad R. Weisbrod, Veronika Michalkova, Marcela Nouzova, Fernando G. Noriega, Francisco Fernandez-Lima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15d7fbfb3cdb411795c683ffebff4309
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spelling	oai:doaj.org-article:15d7fbfb3cdb411795c683ffebff43092021-12-02T14:41:52ZFollowing de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage10.1038/s41598-021-89025-62045-2322https://doaj.org/article/15d7fbfb3cdb411795c683ffebff43092021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89025-6https://doaj.org/toc/2045-2322Abstract Understanding the molecular and biochemical basis of egg development is a central topic in mosquito reproductive biology. Lipids are a major source of energy and building blocks for the developing ovarian follicles. Ultra-High Resolution Mass Spectrometry (UHRMS) combined with in vivo metabolic labeling of follicle lipids with deuterated water (2H2O) can provide unequivocal identification of de novo lipid species during ovarian development. In the present study, we followed de novo triglyceride (TG) dynamics during the ovarian previtellogenic (PVG) stage (2–7 days post-eclosion) of female adult Aedes aegypti. The incorporation of stable isotopes from the diet was evaluated using liquid chromatography (LC) in tandem with the high accuracy (< 0.3 ppm) and high mass resolution (over 1 M) of a 14.5 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (14.5 T FT-ICR MS) equipped with hexapolar detection. LC-UHRMS provides effective lipid class separation and chemical formula identification based on the isotopic fine structure. The monitoring of stable isotope incorporation into de novo incorporated TGs suggests that ovarian lipids are consumed or recycled during the PVG stage, with variable time dynamics. These results provide further evidence of the complexity of the molecular mechanism of follicular lipid dynamics during oogenesis in mosquitoes.Lilian Valadares ToseChad R. WeisbrodVeronika MichalkovaMarcela NouzovaFernando G. NoriegaFrancisco Fernandez-LimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lilian Valadares Tose Chad R. Weisbrod Veronika Michalkova Marcela Nouzova Fernando G. Noriega Francisco Fernandez-Lima Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage
description	Abstract Understanding the molecular and biochemical basis of egg development is a central topic in mosquito reproductive biology. Lipids are a major source of energy and building blocks for the developing ovarian follicles. Ultra-High Resolution Mass Spectrometry (UHRMS) combined with in vivo metabolic labeling of follicle lipids with deuterated water (2H2O) can provide unequivocal identification of de novo lipid species during ovarian development. In the present study, we followed de novo triglyceride (TG) dynamics during the ovarian previtellogenic (PVG) stage (2–7 days post-eclosion) of female adult Aedes aegypti. The incorporation of stable isotopes from the diet was evaluated using liquid chromatography (LC) in tandem with the high accuracy (< 0.3 ppm) and high mass resolution (over 1 M) of a 14.5 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (14.5 T FT-ICR MS) equipped with hexapolar detection. LC-UHRMS provides effective lipid class separation and chemical formula identification based on the isotopic fine structure. The monitoring of stable isotope incorporation into de novo incorporated TGs suggests that ovarian lipids are consumed or recycled during the PVG stage, with variable time dynamics. These results provide further evidence of the complexity of the molecular mechanism of follicular lipid dynamics during oogenesis in mosquitoes.
format	article
author	Lilian Valadares Tose Chad R. Weisbrod Veronika Michalkova Marcela Nouzova Fernando G. Noriega Francisco Fernandez-Lima
author_facet	Lilian Valadares Tose Chad R. Weisbrod Veronika Michalkova Marcela Nouzova Fernando G. Noriega Francisco Fernandez-Lima
author_sort	Lilian Valadares Tose
title	Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage
title_short	Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage
title_full	Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage
title_fullStr	Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage
title_full_unstemmed	Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage
title_sort	following de novo triglyceride dynamics in ovaries of aedes aegypti during the previtellogenic stage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/15d7fbfb3cdb411795c683ffebff4309
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Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage

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