Highly Efficient MicroRNA Delivery Using Functionalized Carbon Dots for Enhanced Conversion of Fibroblasts to Cardiomyocytes

Lei Yang, Song Xue, Mingjun Du, Feng Lian Department of Cardiovascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, People’s Republic of ChinaCorrespondence: Feng Lian Email dr.lianfeng@hotmail.comIntroduction: The reprogramming of induced c...

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Autores principales:	Yang L, Xue S, Du M, Lian F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	nitrogen-enriched carbon dots branched polyethyleneimine micrornas direct reprogramming induced cardiomyocytes Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/15d9455fb7054bdb880c59c7bc7c4d94
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spelling	oai:doaj.org-article:15d9455fb7054bdb880c59c7bc7c4d942021-12-02T18:22:12ZHighly Efficient MicroRNA Delivery Using Functionalized Carbon Dots for Enhanced Conversion of Fibroblasts to Cardiomyocytes1178-2013https://doaj.org/article/15d9455fb7054bdb880c59c7bc7c4d942021-06-01T00:00:00Zhttps://www.dovepress.com/highly-efficient-microrna-delivery-using-functionalized-carbon-dots-fo-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Lei Yang, Song Xue, Mingjun Du, Feng Lian Department of Cardiovascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, People’s Republic of ChinaCorrespondence: Feng Lian Email dr.lianfeng@hotmail.comIntroduction: The reprogramming of induced cardiomyocytes (iCMs) is of particular significance in regenerative medicine; however, it remains a great challenge to fabricate an efficient and safe gene delivery system to induce reprogramming of iCMs for therapeutic applications in heart injury. Here, we report branched polyethyleneimine (BP) coated nitrogen-enriched carbon dots (BP-NCDs) as highly efficient nanocarriers loaded with microRNAs-combo (BP-NCDs/MC) for cardiac reprogramming.Methods: The BP-NCDs nanocarriers were prepared and characterized by several analytical techniques.Results: The BP-NCDs nanocarriers showed good microRNAs-combo binding affinity, negligible cytotoxicity, and long-term microRNAs expression. Importantly, BP-NCDs/MC nanocomplexes led to the efficient direct reprogramming of fibroblasts into iCMs without genomic integration and resulting in effective recovery of cardiac function after myocardial infarction (MI).Conclusion: This study offers a novel strategy to provide safe and effective microRNAs-delivery nanoplatforms based on carbon dots for promising cardiac regeneration and disease therapy.Keywords: nitrogen-enriched carbon dots, branched polyethyleneimine, microRNAs, direct reprogramming, induced cardiomyocytesYang LXue SDu MLian FDove Medical Pressarticlenitrogen-enriched carbon dotsbranched polyethyleneiminemicrornasdirect reprogramminginduced cardiomyocytesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 3741-3754 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nitrogen-enriched carbon dots branched polyethyleneimine micrornas direct reprogramming induced cardiomyocytes Medicine (General) R5-920
spellingShingle	nitrogen-enriched carbon dots branched polyethyleneimine micrornas direct reprogramming induced cardiomyocytes Medicine (General) R5-920 Yang L Xue S Du M Lian F Highly Efficient MicroRNA Delivery Using Functionalized Carbon Dots for Enhanced Conversion of Fibroblasts to Cardiomyocytes
description	Lei Yang, Song Xue, Mingjun Du, Feng Lian Department of Cardiovascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, People’s Republic of ChinaCorrespondence: Feng Lian Email dr.lianfeng@hotmail.comIntroduction: The reprogramming of induced cardiomyocytes (iCMs) is of particular significance in regenerative medicine; however, it remains a great challenge to fabricate an efficient and safe gene delivery system to induce reprogramming of iCMs for therapeutic applications in heart injury. Here, we report branched polyethyleneimine (BP) coated nitrogen-enriched carbon dots (BP-NCDs) as highly efficient nanocarriers loaded with microRNAs-combo (BP-NCDs/MC) for cardiac reprogramming.Methods: The BP-NCDs nanocarriers were prepared and characterized by several analytical techniques.Results: The BP-NCDs nanocarriers showed good microRNAs-combo binding affinity, negligible cytotoxicity, and long-term microRNAs expression. Importantly, BP-NCDs/MC nanocomplexes led to the efficient direct reprogramming of fibroblasts into iCMs without genomic integration and resulting in effective recovery of cardiac function after myocardial infarction (MI).Conclusion: This study offers a novel strategy to provide safe and effective microRNAs-delivery nanoplatforms based on carbon dots for promising cardiac regeneration and disease therapy.Keywords: nitrogen-enriched carbon dots, branched polyethyleneimine, microRNAs, direct reprogramming, induced cardiomyocytes
format	article
author	Yang L Xue S Du M Lian F
author_facet	Yang L Xue S Du M Lian F
author_sort	Yang L
title	Highly Efficient MicroRNA Delivery Using Functionalized Carbon Dots for Enhanced Conversion of Fibroblasts to Cardiomyocytes
title_short	Highly Efficient MicroRNA Delivery Using Functionalized Carbon Dots for Enhanced Conversion of Fibroblasts to Cardiomyocytes
title_full	Highly Efficient MicroRNA Delivery Using Functionalized Carbon Dots for Enhanced Conversion of Fibroblasts to Cardiomyocytes
title_fullStr	Highly Efficient MicroRNA Delivery Using Functionalized Carbon Dots for Enhanced Conversion of Fibroblasts to Cardiomyocytes
title_full_unstemmed	Highly Efficient MicroRNA Delivery Using Functionalized Carbon Dots for Enhanced Conversion of Fibroblasts to Cardiomyocytes
title_sort	highly efficient microrna delivery using functionalized carbon dots for enhanced conversion of fibroblasts to cardiomyocytes
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/15d9455fb7054bdb880c59c7bc7c4d94
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_version_	1718378139147567104

Highly Efficient MicroRNA Delivery Using Functionalized Carbon Dots for Enhanced Conversion of Fibroblasts to Cardiomyocytes

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