Unique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids.
Liver sinusoidal endothelium is strategically positioned to control access of fluids, macromolecules and cells to the liver parenchyma and to serve clearance functions upstream of the hepatocytes. While clearance of macromolecular debris from the peripheral blood is performed by liver sinusoidal end...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/15d96668ffc04350a826ba56f280c3d8 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:15d96668ffc04350a826ba56f280c3d8 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:15d96668ffc04350a826ba56f280c3d82021-11-18T07:23:24ZUnique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids.1932-620310.1371/journal.pone.0034206https://doaj.org/article/15d96668ffc04350a826ba56f280c3d82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22509281/?tool=EBIhttps://doaj.org/toc/1932-6203Liver sinusoidal endothelium is strategically positioned to control access of fluids, macromolecules and cells to the liver parenchyma and to serve clearance functions upstream of the hepatocytes. While clearance of macromolecular debris from the peripheral blood is performed by liver sinusoidal endothelial cells (LSECs) using a delicate endocytic receptor system featuring stabilin-1 and -2, the mannose receptor and CD32b, vascular permeability and cell trafficking are controlled by transcellular pores, i.e. the fenestrae, and by intercellular junctional complexes. In contrast to blood vascular and lymphatic endothelial cells in other organs, the junctional complexes of LSECs have not yet been consistently characterized in molecular terms. In a comprehensive analysis, we here show that LSECs express the typical proteins found in endothelial adherens junctions (AJ), i.e. VE-cadherin as well as α-, β-, p120-catenin and plakoglobin. Tight junction (TJ) transmembrane proteins typical of endothelial cells, i.e. claudin-5 and occludin, were not expressed by rat LSECs while heterogenous immunreactivity for claudin-5 was detected in human LSECs. In contrast, junctional molecules preferentially associating with TJ such as JAM-A, B and C and zonula occludens proteins ZO-1 and ZO-2 were readily detected in LSECs. Remarkably, among the JAMs JAM-C was considerably over-expressed in LSECs as compared to lung microvascular endothelial cells. In conclusion, we show here that LSECs form a special kind of mixed-type intercellular junctions characterized by co-occurrence of endothelial AJ proteins, and of ZO-1 and -2, and JAMs. The distinct molecular architecture of the intercellular junctional complexes of LSECs corroborates previous ultrastructural findings and provides the molecular basis for further analyses of the endothelial barrier function of liver sinusoids under pathologic conditions ranging from hepatic inflammation to formation of liver metastasis.Cyrill GéraudKonstantin EvdokimovBeate K StraubWiebke K PeitschAlexandra DemoryYvette DörflingerKai SchledzewskiAstrid SchmiederPeter SchemmerHellmut G AugustinPeter SchirmacherSergij GoerdtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34206 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Cyrill Géraud Konstantin Evdokimov Beate K Straub Wiebke K Peitsch Alexandra Demory Yvette Dörflinger Kai Schledzewski Astrid Schmieder Peter Schemmer Hellmut G Augustin Peter Schirmacher Sergij Goerdt Unique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids. |
| description |
Liver sinusoidal endothelium is strategically positioned to control access of fluids, macromolecules and cells to the liver parenchyma and to serve clearance functions upstream of the hepatocytes. While clearance of macromolecular debris from the peripheral blood is performed by liver sinusoidal endothelial cells (LSECs) using a delicate endocytic receptor system featuring stabilin-1 and -2, the mannose receptor and CD32b, vascular permeability and cell trafficking are controlled by transcellular pores, i.e. the fenestrae, and by intercellular junctional complexes. In contrast to blood vascular and lymphatic endothelial cells in other organs, the junctional complexes of LSECs have not yet been consistently characterized in molecular terms. In a comprehensive analysis, we here show that LSECs express the typical proteins found in endothelial adherens junctions (AJ), i.e. VE-cadherin as well as α-, β-, p120-catenin and plakoglobin. Tight junction (TJ) transmembrane proteins typical of endothelial cells, i.e. claudin-5 and occludin, were not expressed by rat LSECs while heterogenous immunreactivity for claudin-5 was detected in human LSECs. In contrast, junctional molecules preferentially associating with TJ such as JAM-A, B and C and zonula occludens proteins ZO-1 and ZO-2 were readily detected in LSECs. Remarkably, among the JAMs JAM-C was considerably over-expressed in LSECs as compared to lung microvascular endothelial cells. In conclusion, we show here that LSECs form a special kind of mixed-type intercellular junctions characterized by co-occurrence of endothelial AJ proteins, and of ZO-1 and -2, and JAMs. The distinct molecular architecture of the intercellular junctional complexes of LSECs corroborates previous ultrastructural findings and provides the molecular basis for further analyses of the endothelial barrier function of liver sinusoids under pathologic conditions ranging from hepatic inflammation to formation of liver metastasis. |
| format |
article |
| author |
Cyrill Géraud Konstantin Evdokimov Beate K Straub Wiebke K Peitsch Alexandra Demory Yvette Dörflinger Kai Schledzewski Astrid Schmieder Peter Schemmer Hellmut G Augustin Peter Schirmacher Sergij Goerdt |
| author_facet |
Cyrill Géraud Konstantin Evdokimov Beate K Straub Wiebke K Peitsch Alexandra Demory Yvette Dörflinger Kai Schledzewski Astrid Schmieder Peter Schemmer Hellmut G Augustin Peter Schirmacher Sergij Goerdt |
| author_sort |
Cyrill Géraud |
| title |
Unique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids. |
| title_short |
Unique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids. |
| title_full |
Unique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids. |
| title_fullStr |
Unique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids. |
| title_full_unstemmed |
Unique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids. |
| title_sort |
unique cell type-specific junctional complexes in vascular endothelium of human and rat liver sinusoids. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/15d96668ffc04350a826ba56f280c3d8 |
| work_keys_str_mv |
AT cyrillgeraud uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT konstantinevdokimov uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT beatekstraub uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT wiebkekpeitsch uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT alexandrademory uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT yvettedorflinger uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT kaischledzewski uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT astridschmieder uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT peterschemmer uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT hellmutgaugustin uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT peterschirmacher uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids AT sergijgoerdt uniquecelltypespecificjunctionalcomplexesinvascularendotheliumofhumanandratliversinusoids |
| _version_ |
1718423519827591168 |