Diagnosis of Streptococcus pneumoniae infection using circulating antibody secreting cells

<h4>Background</h4> Streptococcus pneumoniae infections cause morbidity and mortality worldwide. A rapid, simple diagnostic method could reduce the time needed to introduce definitive therapy potentially improving patient outcomes. <h4>Methods</h4> We introduce two new method...

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Autores principales:	Shuya Kyu, Richard P. Ramonell, Merin Kuruvilla, Colleen S. Kraft, Yun F. Wang, Ann R. Falsey, Edward E. Walsh, John L. Daiss, Simon Paulos, Gowrisankar Rajam, Hao Wu, Srinivasan Velusamy, F. Eun-Hyung Lee
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15e224246af34ae5945e28ffa76e7365
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spelling	oai:doaj.org-article:15e224246af34ae5945e28ffa76e73652021-11-25T06:11:01ZDiagnosis of Streptococcus pneumoniae infection using circulating antibody secreting cells1932-6203https://doaj.org/article/15e224246af34ae5945e28ffa76e73652021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589192/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4> Streptococcus pneumoniae infections cause morbidity and mortality worldwide. A rapid, simple diagnostic method could reduce the time needed to introduce definitive therapy potentially improving patient outcomes. <h4>Methods</h4> We introduce two new methods for diagnosing S. pneumoniae infections by measuring the presence of newly activated, pathogen-specific, circulating Antibody Secreting Cells (ASC). First, ASC were detected by ELISpot assays that measure cells secreting antibodies specific for signature antigens. Second, the antibodies secreted by isolated ASC were collected in vitro in a novel matrix, MENSA (media enriched with newly synthesized antibodies) and antibodies against S. pneumoniae antigens were measured using Luminex immunoassays. Each assay was evaluated using blood from S. pneumoniae and non-S. pneumoniae-infected adult patients. <h4>Results</h4> We enrolled 23 patients with culture-confirmed S. pneumoniae infections and 24 controls consisting of 12 non-S. pneumoniae infections, 10 healthy donors and two colonized with S. pneumoniae. By ELISpot assays, twenty-one of 23 infected patients were positive, and all 24 controls were negative. Using MENSA samples, four of five S. pneumoniae-infected patients were positive by Luminex immunoassays while all five non-S. pneumoniae-infected patients were negative. <h4>Conclusion</h4> Specific antibodies produced by activated ASC may provide a simple diagnostic for ongoing S. pneumoniae infections. This method has the potential to diagnose acute bacterial infections.Shuya KyuRichard P. RamonellMerin KuruvillaColleen S. KraftYun F. WangAnn R. FalseyEdward E. WalshJohn L. DaissSimon PaulosGowrisankar RajamHao WuSrinivasan VelusamyF. Eun-Hyung LeePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuya Kyu Richard P. Ramonell Merin Kuruvilla Colleen S. Kraft Yun F. Wang Ann R. Falsey Edward E. Walsh John L. Daiss Simon Paulos Gowrisankar Rajam Hao Wu Srinivasan Velusamy F. Eun-Hyung Lee Diagnosis of Streptococcus pneumoniae infection using circulating antibody secreting cells
description	<h4>Background</h4> Streptococcus pneumoniae infections cause morbidity and mortality worldwide. A rapid, simple diagnostic method could reduce the time needed to introduce definitive therapy potentially improving patient outcomes. <h4>Methods</h4> We introduce two new methods for diagnosing S. pneumoniae infections by measuring the presence of newly activated, pathogen-specific, circulating Antibody Secreting Cells (ASC). First, ASC were detected by ELISpot assays that measure cells secreting antibodies specific for signature antigens. Second, the antibodies secreted by isolated ASC were collected in vitro in a novel matrix, MENSA (media enriched with newly synthesized antibodies) and antibodies against S. pneumoniae antigens were measured using Luminex immunoassays. Each assay was evaluated using blood from S. pneumoniae and non-S. pneumoniae-infected adult patients. <h4>Results</h4> We enrolled 23 patients with culture-confirmed S. pneumoniae infections and 24 controls consisting of 12 non-S. pneumoniae infections, 10 healthy donors and two colonized with S. pneumoniae. By ELISpot assays, twenty-one of 23 infected patients were positive, and all 24 controls were negative. Using MENSA samples, four of five S. pneumoniae-infected patients were positive by Luminex immunoassays while all five non-S. pneumoniae-infected patients were negative. <h4>Conclusion</h4> Specific antibodies produced by activated ASC may provide a simple diagnostic for ongoing S. pneumoniae infections. This method has the potential to diagnose acute bacterial infections.
format	article
author	Shuya Kyu Richard P. Ramonell Merin Kuruvilla Colleen S. Kraft Yun F. Wang Ann R. Falsey Edward E. Walsh John L. Daiss Simon Paulos Gowrisankar Rajam Hao Wu Srinivasan Velusamy F. Eun-Hyung Lee
author_facet	Shuya Kyu Richard P. Ramonell Merin Kuruvilla Colleen S. Kraft Yun F. Wang Ann R. Falsey Edward E. Walsh John L. Daiss Simon Paulos Gowrisankar Rajam Hao Wu Srinivasan Velusamy F. Eun-Hyung Lee
author_sort	Shuya Kyu
title	Diagnosis of Streptococcus pneumoniae infection using circulating antibody secreting cells
title_short	Diagnosis of Streptococcus pneumoniae infection using circulating antibody secreting cells
title_full	Diagnosis of Streptococcus pneumoniae infection using circulating antibody secreting cells
title_fullStr	Diagnosis of Streptococcus pneumoniae infection using circulating antibody secreting cells
title_full_unstemmed	Diagnosis of Streptococcus pneumoniae infection using circulating antibody secreting cells
title_sort	diagnosis of streptococcus pneumoniae infection using circulating antibody secreting cells
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/15e224246af34ae5945e28ffa76e7365
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Diagnosis of Streptococcus pneumoniae infection using circulating antibody secreting cells

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