Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat

Abstract Water-deficit stress negatively affects wheat yield and quality. Abiotic stress on parental plants during reproduction may have transgenerational effects on progeny. Here we investigated the transgenerational influence of pre-anthesis water-deficit stress by detailed analysis of the yield c...

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Autores principales:	Haipei Liu, Amanda J. Able, Jason A. Able
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15e6c4c9d37c4ef891a8714286579dca
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spelling	oai:doaj.org-article:15e6c4c9d37c4ef891a8714286579dca2021-12-02T12:09:18ZSmall RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat10.1038/s41598-021-83074-72045-2322https://doaj.org/article/15e6c4c9d37c4ef891a8714286579dca2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83074-7https://doaj.org/toc/2045-2322Abstract Water-deficit stress negatively affects wheat yield and quality. Abiotic stress on parental plants during reproduction may have transgenerational effects on progeny. Here we investigated the transgenerational influence of pre-anthesis water-deficit stress by detailed analysis of the yield components, grain quality traits, and physiological traits in durum wheat. Next-generation sequencing analysis profiled the small RNA-omics, mRNA transcriptomics, and mRNA degradomics in first generation progeny. Parental water-deficit stress had positive impacts on the progeny for traits including harvest index and protein content in the less stress-tolerant variety. Small RNA-seq identified 1739 conserved and 774 novel microRNAs (miRNAs). Transcriptome-seq characterised the expression of 66,559 genes while degradome-seq profiled the miRNA-guided mRNA cleavage dynamics. Differentially expressed miRNAs and genes were identified, with significant regulatory patterns subject to trans- and inter-generational stress. Integrated analysis using three omics platforms revealed significant biological interactions between stress-responsive miRNA and targets, with transgenerational stress tolerance potentially contributed via pathways such as hormone signalling and nutrient metabolism. Our study provides the first confirmation of the transgenerational effects of water-deficit stress in durum wheat. New insights gained at the molecular level indicate that key miRNA-mRNA modules are candidates for transgenerational stress improvement.Haipei LiuAmanda J. AbleJason A. AbleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haipei Liu Amanda J. Able Jason A. Able Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat
description	Abstract Water-deficit stress negatively affects wheat yield and quality. Abiotic stress on parental plants during reproduction may have transgenerational effects on progeny. Here we investigated the transgenerational influence of pre-anthesis water-deficit stress by detailed analysis of the yield components, grain quality traits, and physiological traits in durum wheat. Next-generation sequencing analysis profiled the small RNA-omics, mRNA transcriptomics, and mRNA degradomics in first generation progeny. Parental water-deficit stress had positive impacts on the progeny for traits including harvest index and protein content in the less stress-tolerant variety. Small RNA-seq identified 1739 conserved and 774 novel microRNAs (miRNAs). Transcriptome-seq characterised the expression of 66,559 genes while degradome-seq profiled the miRNA-guided mRNA cleavage dynamics. Differentially expressed miRNAs and genes were identified, with significant regulatory patterns subject to trans- and inter-generational stress. Integrated analysis using three omics platforms revealed significant biological interactions between stress-responsive miRNA and targets, with transgenerational stress tolerance potentially contributed via pathways such as hormone signalling and nutrient metabolism. Our study provides the first confirmation of the transgenerational effects of water-deficit stress in durum wheat. New insights gained at the molecular level indicate that key miRNA-mRNA modules are candidates for transgenerational stress improvement.
format	article
author	Haipei Liu Amanda J. Able Jason A. Able
author_facet	Haipei Liu Amanda J. Able Jason A. Able
author_sort	Haipei Liu
title	Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat
title_short	Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat
title_full	Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat
title_fullStr	Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat
title_full_unstemmed	Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat
title_sort	small rnas and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/15e6c4c9d37c4ef891a8714286579dca
work_keys_str_mv	AT haipeiliu smallrnasandtheirtargetsareassociatedwiththetransgenerationaleffectsofwaterdeficitstressindurumwheat AT amandajable smallrnasandtheirtargetsareassociatedwiththetransgenerationaleffectsofwaterdeficitstressindurumwheat AT jasonaable smallrnasandtheirtargetsareassociatedwiththetransgenerationaleffectsofwaterdeficitstressindurumwheat
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Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat

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