Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer

Abstract Dysbiosis of the gut microbiome has been associated with the pathogenesis of colorectal cancer (CRC). We profiled the microbiome of gut mucosal tissues from 18 CRC patients and 18 non-CRC controls of the UKM Medical Centre (UKMMC), Kuala Lumpur, Malaysia. The results were then validated usi...

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Autores principales:	Muhammad Afiq Osman, Hui-min Neoh, Nurul-Syakima Ab Mutalib, Siok-Fong Chin, Luqman Mazlan, Raja Affendi Raja Ali, Andee Dzulkarnaen Zakaria, Chai Soon Ngiu, Mia Yang Ang, Rahman Jamal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15e719df30a9400684ffb37e0ac0fee3
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spelling	oai:doaj.org-article:15e719df30a9400684ffb37e0ac0fee32021-12-02T10:44:09ZParvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer10.1038/s41598-021-82465-02045-2322https://doaj.org/article/15e719df30a9400684ffb37e0ac0fee32021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82465-0https://doaj.org/toc/2045-2322Abstract Dysbiosis of the gut microbiome has been associated with the pathogenesis of colorectal cancer (CRC). We profiled the microbiome of gut mucosal tissues from 18 CRC patients and 18 non-CRC controls of the UKM Medical Centre (UKMMC), Kuala Lumpur, Malaysia. The results were then validated using a species-specific quantitative PCR in 40 CRC and 20 non-CRC tissues samples from the UMBI-UKMMC Biobank. Parvimonas micra, Fusobacterium nucleatum, Peptostreptococcus stomatis and Akkermansia muciniphila were found to be over-represented in our CRC patients compared to non-CRC controls. These four bacteria markers distinguished CRC from controls (AUROC = 0.925) in our validation cohort. We identified bacteria species significantly associated (cut-off value of > 5 fold abundance) with various CRC demographics such as ethnicity, gender and CRC staging; however, due to small sample size of the discovery cohort, these results could not be further verified in our validation cohort. In summary, Parvimonas micra, Fusobacterium nucleatum, Peptostreptococcus stomatis and Akkermansia muciniphila were enriched in our local CRC patients. Nevertheless, the roles of these bacteria in CRC initiation and progression remains to be investigated.Muhammad Afiq OsmanHui-min NeohNurul-Syakima Ab MutalibSiok-Fong ChinLuqman MazlanRaja Affendi Raja AliAndee Dzulkarnaen ZakariaChai Soon NgiuMia Yang AngRahman JamalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muhammad Afiq Osman Hui-min Neoh Nurul-Syakima Ab Mutalib Siok-Fong Chin Luqman Mazlan Raja Affendi Raja Ali Andee Dzulkarnaen Zakaria Chai Soon Ngiu Mia Yang Ang Rahman Jamal Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
description	Abstract Dysbiosis of the gut microbiome has been associated with the pathogenesis of colorectal cancer (CRC). We profiled the microbiome of gut mucosal tissues from 18 CRC patients and 18 non-CRC controls of the UKM Medical Centre (UKMMC), Kuala Lumpur, Malaysia. The results were then validated using a species-specific quantitative PCR in 40 CRC and 20 non-CRC tissues samples from the UMBI-UKMMC Biobank. Parvimonas micra, Fusobacterium nucleatum, Peptostreptococcus stomatis and Akkermansia muciniphila were found to be over-represented in our CRC patients compared to non-CRC controls. These four bacteria markers distinguished CRC from controls (AUROC = 0.925) in our validation cohort. We identified bacteria species significantly associated (cut-off value of > 5 fold abundance) with various CRC demographics such as ethnicity, gender and CRC staging; however, due to small sample size of the discovery cohort, these results could not be further verified in our validation cohort. In summary, Parvimonas micra, Fusobacterium nucleatum, Peptostreptococcus stomatis and Akkermansia muciniphila were enriched in our local CRC patients. Nevertheless, the roles of these bacteria in CRC initiation and progression remains to be investigated.
format	article
author	Muhammad Afiq Osman Hui-min Neoh Nurul-Syakima Ab Mutalib Siok-Fong Chin Luqman Mazlan Raja Affendi Raja Ali Andee Dzulkarnaen Zakaria Chai Soon Ngiu Mia Yang Ang Rahman Jamal
author_facet	Muhammad Afiq Osman Hui-min Neoh Nurul-Syakima Ab Mutalib Siok-Fong Chin Luqman Mazlan Raja Affendi Raja Ali Andee Dzulkarnaen Zakaria Chai Soon Ngiu Mia Yang Ang Rahman Jamal
author_sort	Muhammad Afiq Osman
title	Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title_short	Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title_full	Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title_fullStr	Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title_full_unstemmed	Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title_sort	parvimonas micra, peptostreptococcus stomatis, fusobacterium nucleatum and akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/15e719df30a9400684ffb37e0ac0fee3
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Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer

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