Association between the Three Polymorphisms of the Glucocorticoid Receptor Gene and the Early Clinical Outcome in Kidney Transplantation Patients

Background: Glucocorticoids are pivotal components of immunosuppressive regimens in solid organ transplantations. This study aimed to assess the possible association between the ER22/23EK, N363S, and Bcl1 polymorphisms, and short-term clinical outcomes, including acute rejection and delayed graft fu...

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Autores principales:	Shaghayegh Mottaghi, Mohammad Mahdi Sagheb, Negar Azarpira, Faezeh Abdizadeh, Romina Faeghi, Iman Karimzadeh
Formato:	article
Lenguaje:	EN
Publicado:	Shiraz University of Medical Sciences 2021
Materias:	kidney transplantation receptors glucocorticoids polymorphism genetic Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/15e9455d75a44ada89f12896dd553242
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spelling	oai:doaj.org-article:15e9455d75a44ada89f12896dd5532422021-11-09T06:23:38ZAssociation between the Three Polymorphisms of the Glucocorticoid Receptor Gene and the Early Clinical Outcome in Kidney Transplantation Patients0253-07161735-368810.30476/ijms.2020.85872.1550https://doaj.org/article/15e9455d75a44ada89f12896dd5532422021-11-01T00:00:00Zhttps://ijms.sums.ac.ir/article_47325_49f89c2517acaa489e6a708ae5d6b0db.pdfhttps://doaj.org/toc/0253-0716https://doaj.org/toc/1735-3688Background: Glucocorticoids are pivotal components of immunosuppressive regimens in solid organ transplantations. This study aimed to assess the possible association between the ER22/23EK, N363S, and Bcl1 polymorphisms, and short-term clinical outcomes, including acute rejection and delayed graft function (DGF), in kidney transplantation recipients. Methods: A case-control study was conducted in a two-year period on adults with transplanted kidneys, comprised of subjects without rejection (n=50, control) and those with documented rejection within one year after transplantation (n=50, case), between April 2017 and September 2018, in Shiraz, Iran. Demographic characteristics and clinical and paraclinical findings were gathered. The genotyping of the ER22/23EK, N363S, and Bcl1 polymorphisms was carried out via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The association between the genotypes and DGF as well as rejection types was evaluated using either the Chi square test or Fisher exact test. A stepwise logistic regression analysis was conducted to determine the independent factors of acute rejection within the first year after transplantation. Results: The study population consisted of 64 men and 36 women. The frequency of mutated alleles was 0.32 for G (Bcl1), 0.02 for S (N363S), and 0.065 for A (ER22/23EK). There was no significant association either between the studied polymorphisms and acute rejection or between the Bcl1 (P=0.17), N363S (P=0.99), and ER22/23EK (P=0.99) genotypes and DGF. The length of hospital stay after kidney transplantation was slightly more in N363N and ER22/23EK wild allele carriers. However, this difference was not statistically significant.Conclusion: Our data suggested no statistically significant association between the genotypes of the studied polymorphisms and early clinical outcomes after kidney transplantation.Shaghayegh MottaghiMohammad Mahdi SaghebNegar AzarpiraFaezeh AbdizadehRomina FaeghiIman KarimzadehShiraz University of Medical Sciencesarticlekidney transplantationreceptorsglucocorticoidspolymorphismgeneticMedicine (General)R5-920ENIranian Journal of Medical Sciences, Vol 46, Iss 6, Pp 444-453 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	kidney transplantation receptors glucocorticoids polymorphism genetic Medicine (General) R5-920
spellingShingle	kidney transplantation receptors glucocorticoids polymorphism genetic Medicine (General) R5-920 Shaghayegh Mottaghi Mohammad Mahdi Sagheb Negar Azarpira Faezeh Abdizadeh Romina Faeghi Iman Karimzadeh Association between the Three Polymorphisms of the Glucocorticoid Receptor Gene and the Early Clinical Outcome in Kidney Transplantation Patients
description	Background: Glucocorticoids are pivotal components of immunosuppressive regimens in solid organ transplantations. This study aimed to assess the possible association between the ER22/23EK, N363S, and Bcl1 polymorphisms, and short-term clinical outcomes, including acute rejection and delayed graft function (DGF), in kidney transplantation recipients. Methods: A case-control study was conducted in a two-year period on adults with transplanted kidneys, comprised of subjects without rejection (n=50, control) and those with documented rejection within one year after transplantation (n=50, case), between April 2017 and September 2018, in Shiraz, Iran. Demographic characteristics and clinical and paraclinical findings were gathered. The genotyping of the ER22/23EK, N363S, and Bcl1 polymorphisms was carried out via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The association between the genotypes and DGF as well as rejection types was evaluated using either the Chi square test or Fisher exact test. A stepwise logistic regression analysis was conducted to determine the independent factors of acute rejection within the first year after transplantation. Results: The study population consisted of 64 men and 36 women. The frequency of mutated alleles was 0.32 for G (Bcl1), 0.02 for S (N363S), and 0.065 for A (ER22/23EK). There was no significant association either between the studied polymorphisms and acute rejection or between the Bcl1 (P=0.17), N363S (P=0.99), and ER22/23EK (P=0.99) genotypes and DGF. The length of hospital stay after kidney transplantation was slightly more in N363N and ER22/23EK wild allele carriers. However, this difference was not statistically significant.Conclusion: Our data suggested no statistically significant association between the genotypes of the studied polymorphisms and early clinical outcomes after kidney transplantation.
format	article
author	Shaghayegh Mottaghi Mohammad Mahdi Sagheb Negar Azarpira Faezeh Abdizadeh Romina Faeghi Iman Karimzadeh
author_facet	Shaghayegh Mottaghi Mohammad Mahdi Sagheb Negar Azarpira Faezeh Abdizadeh Romina Faeghi Iman Karimzadeh
author_sort	Shaghayegh Mottaghi
title	Association between the Three Polymorphisms of the Glucocorticoid Receptor Gene and the Early Clinical Outcome in Kidney Transplantation Patients
title_short	Association between the Three Polymorphisms of the Glucocorticoid Receptor Gene and the Early Clinical Outcome in Kidney Transplantation Patients
title_full	Association between the Three Polymorphisms of the Glucocorticoid Receptor Gene and the Early Clinical Outcome in Kidney Transplantation Patients
title_fullStr	Association between the Three Polymorphisms of the Glucocorticoid Receptor Gene and the Early Clinical Outcome in Kidney Transplantation Patients
title_full_unstemmed	Association between the Three Polymorphisms of the Glucocorticoid Receptor Gene and the Early Clinical Outcome in Kidney Transplantation Patients
title_sort	association between the three polymorphisms of the glucocorticoid receptor gene and the early clinical outcome in kidney transplantation patients
publisher	Shiraz University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/15e9455d75a44ada89f12896dd553242
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Association between the Three Polymorphisms of the Glucocorticoid Receptor Gene and the Early Clinical Outcome in Kidney Transplantation Patients

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