Transmission of Escherichia coli from Manure to Root Zones of Field-Grown Lettuce and Leek Plants

Pathogenic Escherichia coli strains are responsible for food-borne disease outbreaks upon consumption of fresh vegetables and fruits. The aim of this study was to establish the transmission route of E. coli strain 0611, as proxy for human pathogenic E....

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Autores principales:	Leo van Overbeek, Marie Duhamel, Stefan Aanstoot, Carin Lombaers van der Plas, Els Nijhuis, Leo Poleij, Lina Russ, Patricia van der Zouwen, Beatriz Andreo-Jimenez
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Escherichia coli</i> plants manure transmission food safety metagenome Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/15ebc1982a3a4dce836f664eaef2ea4a
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spelling	oai:doaj.org-article:15ebc1982a3a4dce836f664eaef2ea4a2021-11-25T18:24:52ZTransmission of <i>Escherichia coli</i> from Manure to Root Zones of Field-Grown Lettuce and Leek Plants10.3390/microorganisms91122892076-2607https://doaj.org/article/15ebc1982a3a4dce836f664eaef2ea4a2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2289https://doaj.org/toc/2076-2607Pathogenic <i>Escherichia coli</i> strains are responsible for food-borne disease outbreaks upon consumption of fresh vegetables and fruits. The aim of this study was to establish the transmission route of <i>E. coli</i> strain 0611, as proxy for human pathogenic <i>E. coli</i>, via manure, soil and plant root zones to the above-soil plant compartments. The ecological behavior of the introduced strain was established by making use of a combination of cultivation-based and molecular targeted and untargeted approaches. Strain 0611 CFUs and specific molecular targets were detected in the root zones of lettuce and leek plants, even up to 272 days after planting in the case of leek plants. However, no strain 0611 colonies were detected in leek leaves, and only in one occasion a single colony was found in lettuce leaves. Therefore, it was concluded that transmission of <i>E. coli</i> via manure is not the principal contamination route to the edible parts of both plant species grown under field conditions in this study. Strain 0611 was shown to accumulate in root zones of both species and metagenomic reads of this strain were retrieved from the lettuce rhizosphere soil metagenome library at a level of Log 4.11 CFU per g dry soil.Leo van OverbeekMarie DuhamelStefan AanstootCarin Lombaers van der PlasEls NijhuisLeo PoleijLina RussPatricia van der ZouwenBeatriz Andreo-JimenezMDPI AGarticle<i>Escherichia coli</i>plantsmanuretransmissionfood safetymetagenomeBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2289, p 2289 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Escherichia coli</i> plants manure transmission food safety metagenome Biology (General) QH301-705.5
spellingShingle	<i>Escherichia coli</i> plants manure transmission food safety metagenome Biology (General) QH301-705.5 Leo van Overbeek Marie Duhamel Stefan Aanstoot Carin Lombaers van der Plas Els Nijhuis Leo Poleij Lina Russ Patricia van der Zouwen Beatriz Andreo-Jimenez Transmission of <i>Escherichia coli</i> from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
description	Pathogenic <i>Escherichia coli</i> strains are responsible for food-borne disease outbreaks upon consumption of fresh vegetables and fruits. The aim of this study was to establish the transmission route of <i>E. coli</i> strain 0611, as proxy for human pathogenic <i>E. coli</i>, via manure, soil and plant root zones to the above-soil plant compartments. The ecological behavior of the introduced strain was established by making use of a combination of cultivation-based and molecular targeted and untargeted approaches. Strain 0611 CFUs and specific molecular targets were detected in the root zones of lettuce and leek plants, even up to 272 days after planting in the case of leek plants. However, no strain 0611 colonies were detected in leek leaves, and only in one occasion a single colony was found in lettuce leaves. Therefore, it was concluded that transmission of <i>E. coli</i> via manure is not the principal contamination route to the edible parts of both plant species grown under field conditions in this study. Strain 0611 was shown to accumulate in root zones of both species and metagenomic reads of this strain were retrieved from the lettuce rhizosphere soil metagenome library at a level of Log 4.11 CFU per g dry soil.
format	article
author	Leo van Overbeek Marie Duhamel Stefan Aanstoot Carin Lombaers van der Plas Els Nijhuis Leo Poleij Lina Russ Patricia van der Zouwen Beatriz Andreo-Jimenez
author_facet	Leo van Overbeek Marie Duhamel Stefan Aanstoot Carin Lombaers van der Plas Els Nijhuis Leo Poleij Lina Russ Patricia van der Zouwen Beatriz Andreo-Jimenez
author_sort	Leo van Overbeek
title	Transmission of <i>Escherichia coli</i> from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title_short	Transmission of <i>Escherichia coli</i> from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title_full	Transmission of <i>Escherichia coli</i> from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title_fullStr	Transmission of <i>Escherichia coli</i> from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title_full_unstemmed	Transmission of <i>Escherichia coli</i> from Manure to Root Zones of Field-Grown Lettuce and Leek Plants
title_sort	transmission of <i>escherichia coli</i> from manure to root zones of field-grown lettuce and leek plants
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/15ebc1982a3a4dce836f664eaef2ea4a
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Transmission of <i>Escherichia coli</i> from Manure to Root Zones of Field-Grown Lettuce and Leek Plants

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