High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.

<h4>Background</h4>Apple allergy manifests as two main groups of clinical entities reflecting different patterns of allergen sensitization: oral allergy syndrome (OAS) and generalized symptoms (GS).<h4>Objective</h4>We analysed the sensitization profile to a wide panel of dif...

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Autores principales:	Francisca Gomez, Ana Aranda, Paloma Campo, Araceli Diaz-Perales, Natalia Blanca-Lopez, James Perkins, Maria Garrido, Miguel Blanca, Cristobalina Mayorga, Maria José Torres
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15ec3b8ee39f41a48c247c1087282bc2
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spelling	oai:doaj.org-article:15ec3b8ee39f41a48c247c1087282bc22021-11-25T06:01:03ZHigh prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.1932-620310.1371/journal.pone.0107304https://doaj.org/article/15ec3b8ee39f41a48c247c1087282bc22014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107304https://doaj.org/toc/1932-6203<h4>Background</h4>Apple allergy manifests as two main groups of clinical entities reflecting different patterns of allergen sensitization: oral allergy syndrome (OAS) and generalized symptoms (GS).<h4>Objective</h4>We analysed the sensitization profile to a wide panel of different components of food allergens (rMal d 1, Mal d 2, rMal d 3, rMal d 4, rPru p 3, rBet v 1 and Pho d 2) for a population of Mediterranean patients with OAS and GS to apple.<h4>Methods</h4>Patients (N = 81) with a history of apple allergy that could be confirmed by positive prick-prick test and/or double-blind-placebo-controlled food challenge (DBPCFC), were included. Skin prick test (SPT) and ELISA were performed using a panel of inhalant, fruit and nut allergens. ELISA and ELISA inhibition studies were performed in order to analyse the sensitization patterns.<h4>Results</h4>Thirty-five cases (43.2%) had OAS and 46 (56.8%) GS. SPT showed a significantly higher number of positive results with peach, cherry and hazelnut in those with GS. ELISA showed a significantly high percentage of positive cases to rMal d 3, rMal d 4, rPru p 3 and Pho d 2 in patients with OAS and GS compared to controls, and to rBet v 1 in patients with OAS vs controls and between OAS and GS patients. Three different patterns of recognition were detected: positive to LTP (rMal d 3 or rPru p 3), positive to profilin (rMal d 4 and Pho d 2), or positive to both. There were also patients with rMal d 1 recognition who showed cross-reactivity to rBet v 1.<h4>Conclusion</h4>In an apple allergy population with a high incidence of pollinosis different patterns of sensitization may occur. LTP is most often involved in those with GS. Profilin, though more prevalent in patients with OAS, has been shown to sensitise patients with both types of symptoms.Francisca GomezAna ArandaPaloma CampoAraceli Diaz-PeralesNatalia Blanca-LopezJames PerkinsMaria GarridoMaria GarridoMiguel BlancaCristobalina MayorgaMaria José TorresPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107304 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francisca Gomez Ana Aranda Paloma Campo Araceli Diaz-Perales Natalia Blanca-Lopez James Perkins Maria Garrido Maria Garrido Miguel Blanca Cristobalina Mayorga Maria José Torres High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.
description	<h4>Background</h4>Apple allergy manifests as two main groups of clinical entities reflecting different patterns of allergen sensitization: oral allergy syndrome (OAS) and generalized symptoms (GS).<h4>Objective</h4>We analysed the sensitization profile to a wide panel of different components of food allergens (rMal d 1, Mal d 2, rMal d 3, rMal d 4, rPru p 3, rBet v 1 and Pho d 2) for a population of Mediterranean patients with OAS and GS to apple.<h4>Methods</h4>Patients (N = 81) with a history of apple allergy that could be confirmed by positive prick-prick test and/or double-blind-placebo-controlled food challenge (DBPCFC), were included. Skin prick test (SPT) and ELISA were performed using a panel of inhalant, fruit and nut allergens. ELISA and ELISA inhibition studies were performed in order to analyse the sensitization patterns.<h4>Results</h4>Thirty-five cases (43.2%) had OAS and 46 (56.8%) GS. SPT showed a significantly higher number of positive results with peach, cherry and hazelnut in those with GS. ELISA showed a significantly high percentage of positive cases to rMal d 3, rMal d 4, rPru p 3 and Pho d 2 in patients with OAS and GS compared to controls, and to rBet v 1 in patients with OAS vs controls and between OAS and GS patients. Three different patterns of recognition were detected: positive to LTP (rMal d 3 or rPru p 3), positive to profilin (rMal d 4 and Pho d 2), or positive to both. There were also patients with rMal d 1 recognition who showed cross-reactivity to rBet v 1.<h4>Conclusion</h4>In an apple allergy population with a high incidence of pollinosis different patterns of sensitization may occur. LTP is most often involved in those with GS. Profilin, though more prevalent in patients with OAS, has been shown to sensitise patients with both types of symptoms.
format	article
author	Francisca Gomez Ana Aranda Paloma Campo Araceli Diaz-Perales Natalia Blanca-Lopez James Perkins Maria Garrido Maria Garrido Miguel Blanca Cristobalina Mayorga Maria José Torres
author_facet	Francisca Gomez Ana Aranda Paloma Campo Araceli Diaz-Perales Natalia Blanca-Lopez James Perkins Maria Garrido Maria Garrido Miguel Blanca Cristobalina Mayorga Maria José Torres
author_sort	Francisca Gomez
title	High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.
title_short	High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.
title_full	High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.
title_fullStr	High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.
title_full_unstemmed	High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.
title_sort	high prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/15ec3b8ee39f41a48c247c1087282bc2
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High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.

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