<italic toggle="yes">Leishmania</italic> Encodes a Bacterium-like 2,4-Dienoyl-Coenzyme A Reductase That Is Required for Fatty Acid β-Oxidation and Intracellular Parasite Survival

ABSTRACT Leishmania spp. are protozoan parasites that cause a spectrum of important diseases in humans. These parasites develop as extracellular promastigotes in the digestive tract of their insect vectors and as obligate intracellular amastigotes that infect macrophages and other phagocytic cells i...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Geo Semini, Daniel Paape, Martin Blume, M. Fleur Sernee, Diego Peres-Alonso, Sébastien Calvignac-Spencer, Jörg Döllinger, Stefan Jehle, Eleanor Saunders, Malcolm J. McConville, Toni Aebischer
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2020
Materias:
Acceso en línea:https://doaj.org/article/15f077c89e7b4930b189e22c358030ca
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:15f077c89e7b4930b189e22c358030ca
record_format dspace
spelling oai:doaj.org-article:15f077c89e7b4930b189e22c358030ca2021-11-15T15:56:46Z<italic toggle="yes">Leishmania</italic> Encodes a Bacterium-like 2,4-Dienoyl-Coenzyme A Reductase That Is Required for Fatty Acid β-Oxidation and Intracellular Parasite Survival10.1128/mBio.01057-202150-7511https://doaj.org/article/15f077c89e7b4930b189e22c358030ca2020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01057-20https://doaj.org/toc/2150-7511ABSTRACT Leishmania spp. are protozoan parasites that cause a spectrum of important diseases in humans. These parasites develop as extracellular promastigotes in the digestive tract of their insect vectors and as obligate intracellular amastigotes that infect macrophages and other phagocytic cells in their vertebrate hosts. Promastigote-to-amastigote differentiation is associated with marked changes in metabolism, including the upregulation of enzymes involved in fatty acid β-oxidation, which may reflect adaptation to the intracellular niche. Here, we have investigated the function of one of these enzymes, a putative 2,4-dienoyl-coenzyme A (CoA) reductase (DECR), which is specifically required for the β-oxidation of polyunsaturated fatty acids. The Leishmania DECR shows close homology to bacterial DECR proteins, suggesting that it was acquired by lateral gene transfer. It is present in other trypanosomatids that have obligate intracellular stages (i.e., Trypanosoma cruzi and Angomonas) but is absent from dixenous parasites with an exclusively extracellular lifestyle (i.e., Trypanosoma brucei). A DECR-green fluorescent protein (GFP) fusion protein was localized to the mitochondrion in both promastigote and amastigote stages, and the levels of expression increased in the latter stages. A Leishmania major Δdecr null mutant was unable to catabolize unsaturated fatty acids and accumulated the intermediate 2,4-decadienoyl-CoA, confirming DECR’s role in β-oxidation. Strikingly, the L. major Δdecr mutant was unable to survive in macrophages and was avirulent in BALB/c mice. These findings suggest that β-oxidation of polyunsaturated fatty acids is essential for intracellular parasite survival and that the bacterial origin of key enzymes in this pathway could be exploited in developing new therapies. IMPORTANCE The Trypanosomatidae are protozoan parasites that infect insects, plants, and animals and have evolved complex monoxenous (single host) and dixenous (two hosts) lifestyles. A number of species of Trypanosomatidae, including Leishmania spp., have evolved the capacity to survive within intracellular niches in vertebrate hosts. The adaptations, metabolic and other, that are associated with development of intracellular lifestyles remain poorly defined. We show that genomes of Leishmania and Trypanosomatidae that can survive intracellularly encode a 2,4-dienoyl-CoA reductase that is involved in catabolism of a subclass of fatty acids. The trypanosomatid enzyme shows closest similarity to the corresponding bacterial enzymes and is located in the mitochondrion and essential for intracellular growth of Leishmania. The findings suggest that acquisition of this gene by lateral gene transfer from bacteria by ancestral monoxenous Trypanosomatidae likely contributed to the development of a dixenous lifestyle of these parasites.Geo SeminiDaniel PaapeMartin BlumeM. Fleur SerneeDiego Peres-AlonsoSébastien Calvignac-SpencerJörg DöllingerStefan JehleEleanor SaundersMalcolm J. McConvilleToni AebischerAmerican Society for MicrobiologyarticleKinetoplastidalateral gene transfermitochondrial metabolismvirulence factorsMicrobiologyQR1-502ENmBio, Vol 11, Iss 3 (2020)
institution DOAJ
collection DOAJ
language EN
topic Kinetoplastida
lateral gene transfer
mitochondrial metabolism
virulence factors
Microbiology
QR1-502
spellingShingle Kinetoplastida
lateral gene transfer
mitochondrial metabolism
virulence factors
Microbiology
QR1-502
Geo Semini
Daniel Paape
Martin Blume
M. Fleur Sernee
Diego Peres-Alonso
Sébastien Calvignac-Spencer
Jörg Döllinger
Stefan Jehle
Eleanor Saunders
Malcolm J. McConville
Toni Aebischer
<italic toggle="yes">Leishmania</italic> Encodes a Bacterium-like 2,4-Dienoyl-Coenzyme A Reductase That Is Required for Fatty Acid β-Oxidation and Intracellular Parasite Survival
description ABSTRACT Leishmania spp. are protozoan parasites that cause a spectrum of important diseases in humans. These parasites develop as extracellular promastigotes in the digestive tract of their insect vectors and as obligate intracellular amastigotes that infect macrophages and other phagocytic cells in their vertebrate hosts. Promastigote-to-amastigote differentiation is associated with marked changes in metabolism, including the upregulation of enzymes involved in fatty acid β-oxidation, which may reflect adaptation to the intracellular niche. Here, we have investigated the function of one of these enzymes, a putative 2,4-dienoyl-coenzyme A (CoA) reductase (DECR), which is specifically required for the β-oxidation of polyunsaturated fatty acids. The Leishmania DECR shows close homology to bacterial DECR proteins, suggesting that it was acquired by lateral gene transfer. It is present in other trypanosomatids that have obligate intracellular stages (i.e., Trypanosoma cruzi and Angomonas) but is absent from dixenous parasites with an exclusively extracellular lifestyle (i.e., Trypanosoma brucei). A DECR-green fluorescent protein (GFP) fusion protein was localized to the mitochondrion in both promastigote and amastigote stages, and the levels of expression increased in the latter stages. A Leishmania major Δdecr null mutant was unable to catabolize unsaturated fatty acids and accumulated the intermediate 2,4-decadienoyl-CoA, confirming DECR’s role in β-oxidation. Strikingly, the L. major Δdecr mutant was unable to survive in macrophages and was avirulent in BALB/c mice. These findings suggest that β-oxidation of polyunsaturated fatty acids is essential for intracellular parasite survival and that the bacterial origin of key enzymes in this pathway could be exploited in developing new therapies. IMPORTANCE The Trypanosomatidae are protozoan parasites that infect insects, plants, and animals and have evolved complex monoxenous (single host) and dixenous (two hosts) lifestyles. A number of species of Trypanosomatidae, including Leishmania spp., have evolved the capacity to survive within intracellular niches in vertebrate hosts. The adaptations, metabolic and other, that are associated with development of intracellular lifestyles remain poorly defined. We show that genomes of Leishmania and Trypanosomatidae that can survive intracellularly encode a 2,4-dienoyl-CoA reductase that is involved in catabolism of a subclass of fatty acids. The trypanosomatid enzyme shows closest similarity to the corresponding bacterial enzymes and is located in the mitochondrion and essential for intracellular growth of Leishmania. The findings suggest that acquisition of this gene by lateral gene transfer from bacteria by ancestral monoxenous Trypanosomatidae likely contributed to the development of a dixenous lifestyle of these parasites.
format article
author Geo Semini
Daniel Paape
Martin Blume
M. Fleur Sernee
Diego Peres-Alonso
Sébastien Calvignac-Spencer
Jörg Döllinger
Stefan Jehle
Eleanor Saunders
Malcolm J. McConville
Toni Aebischer
author_facet Geo Semini
Daniel Paape
Martin Blume
M. Fleur Sernee
Diego Peres-Alonso
Sébastien Calvignac-Spencer
Jörg Döllinger
Stefan Jehle
Eleanor Saunders
Malcolm J. McConville
Toni Aebischer
author_sort Geo Semini
title <italic toggle="yes">Leishmania</italic> Encodes a Bacterium-like 2,4-Dienoyl-Coenzyme A Reductase That Is Required for Fatty Acid β-Oxidation and Intracellular Parasite Survival
title_short <italic toggle="yes">Leishmania</italic> Encodes a Bacterium-like 2,4-Dienoyl-Coenzyme A Reductase That Is Required for Fatty Acid β-Oxidation and Intracellular Parasite Survival
title_full <italic toggle="yes">Leishmania</italic> Encodes a Bacterium-like 2,4-Dienoyl-Coenzyme A Reductase That Is Required for Fatty Acid β-Oxidation and Intracellular Parasite Survival
title_fullStr <italic toggle="yes">Leishmania</italic> Encodes a Bacterium-like 2,4-Dienoyl-Coenzyme A Reductase That Is Required for Fatty Acid β-Oxidation and Intracellular Parasite Survival
title_full_unstemmed <italic toggle="yes">Leishmania</italic> Encodes a Bacterium-like 2,4-Dienoyl-Coenzyme A Reductase That Is Required for Fatty Acid β-Oxidation and Intracellular Parasite Survival
title_sort <italic toggle="yes">leishmania</italic> encodes a bacterium-like 2,4-dienoyl-coenzyme a reductase that is required for fatty acid β-oxidation and intracellular parasite survival
publisher American Society for Microbiology
publishDate 2020
url https://doaj.org/article/15f077c89e7b4930b189e22c358030ca
work_keys_str_mv AT geosemini italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
AT danielpaape italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
AT martinblume italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
AT mfleursernee italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
AT diegoperesalonso italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
AT sebastiencalvignacspencer italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
AT jorgdollinger italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
AT stefanjehle italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
AT eleanorsaunders italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
AT malcolmjmcconville italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
AT toniaebischer italictoggleyesleishmaniaitalicencodesabacteriumlike24dienoylcoenzymeareductasethatisrequiredforfattyacidboxidationandintracellularparasitesurvival
_version_ 1718427072189169664