Comprehensive Evaluation of Lipopolysaccharide-Induced Changes in Rats Based on Metabolomics

Chunmei Geng,1 Yujin Guo,1 Changshui Wang,2 Changmeng Cui,3 Wenxiu Han,1 Dehua Liao,4 Pei Jiang1 1Department of Pharmacy, Jining First People’s Hospital, Jining Medical University, Jining 272000, People’s Republic of China; 2Department of Clinical Translational Medicine, Jining L...

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Autores principales:	Geng C, Guo Y, Wang C, Cui C, Han W, Liao D, Jiang P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	systemic inflammatory response syndrome metabolites gas chromatography–mass spectrometry multivariate statistical analysis Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/16037bfcc4b543be8bd6d670a5975800
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spelling	oai:doaj.org-article:16037bfcc4b543be8bd6d670a59758002021-12-02T10:25:37ZComprehensive Evaluation of Lipopolysaccharide-Induced Changes in Rats Based on Metabolomics1178-7031https://doaj.org/article/16037bfcc4b543be8bd6d670a59758002020-08-01T00:00:00Zhttps://www.dovepress.com/comprehensive-evaluation-of-lipopolysaccharide-induced-changes-in-rats-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Chunmei Geng,1 Yujin Guo,1 Changshui Wang,2 Changmeng Cui,3 Wenxiu Han,1 Dehua Liao,4 Pei Jiang1 1Department of Pharmacy, Jining First People’s Hospital, Jining Medical University, Jining 272000, People’s Republic of China; 2Department of Clinical Translational Medicine, Jining Life Science Center, Jining 272000, People’s Republic of China; 3Department of Neurosurgery, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining 272000, People’s Republic of China; 4Department of Pharmacy, Hunan Cancer Hospital, Central South University, Changsha 410011, People’s Republic of ChinaCorrespondence: Pei JiangDepartment of Pharmacy, Jining First People’s Hospital, Jining Medical University, Jining 272000, People’s Republic of ChinaTel/Fax +86 537 2106208Email jiangpeicsu@sina.comPurpose: Substantial evidence indicates that lipopolysaccharide (LPS) exposure can lead to systemic inflammatory response syndrome (SIRS) and multiple organ failure. Previous metabolomic studies have mainly focused on LPS-induced depression or hepatic and renal effects. However, no comprehensive metabolomics-based analysis of the serum, liver, kidney, hippocampus, and heart following exposure to LPS has been undertaken to date.Material and Methods: Male Sprague–Dawley rats were randomly allocated to a control and a LPS-treated group (n=8). LPS for 2 weeks (0.5 mg/kg every other day) was given via intraperitoneal injection. Gas chromatography–mass spectrometry (GC–MS) was used for metabolite determination, while multivariate statistical analysis was performed to identify differentially expressed metabolites between the two groups.Results: Our study revealed that 24, 13, 12, 7, and 12 metabolites were differentially expressed between the LPS treatment group and the control group in the serum, liver, kidney, hippocampus, and heart, respectively. We further identified that these metabolic changes were mainly involved with aminoacyl-tRNA biosynthesis; glutathione metabolism; glyoxylate and dicarboxylate metabolism; glycine, serine, and threonine metabolism; arginine biosynthesis; bile acid biosynthesis; and glycerolipid metabolism.Conclusion: We have systematically elucidated the metabolic changes underlying LPS-induced SIRS, thereby providing insight into the mechanisms associated with these alterations.Keywords: systemic inflammatory response syndrome, metabolites, gas chromatography–mass spectrometry, multivariate statistical analysisGeng CGuo YWang CCui CHan WLiao DJiang PDove Medical Pressarticlesystemic inflammatory response syndromemetabolitesgas chromatography–mass spectrometrymultivariate statistical analysisPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 477-486 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	systemic inflammatory response syndrome metabolites gas chromatography–mass spectrometry multivariate statistical analysis Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	systemic inflammatory response syndrome metabolites gas chromatography–mass spectrometry multivariate statistical analysis Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Geng C Guo Y Wang C Cui C Han W Liao D Jiang P Comprehensive Evaluation of Lipopolysaccharide-Induced Changes in Rats Based on Metabolomics
description	Chunmei Geng,1 Yujin Guo,1 Changshui Wang,2 Changmeng Cui,3 Wenxiu Han,1 Dehua Liao,4 Pei Jiang1 1Department of Pharmacy, Jining First People’s Hospital, Jining Medical University, Jining 272000, People’s Republic of China; 2Department of Clinical Translational Medicine, Jining Life Science Center, Jining 272000, People’s Republic of China; 3Department of Neurosurgery, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining 272000, People’s Republic of China; 4Department of Pharmacy, Hunan Cancer Hospital, Central South University, Changsha 410011, People’s Republic of ChinaCorrespondence: Pei JiangDepartment of Pharmacy, Jining First People’s Hospital, Jining Medical University, Jining 272000, People’s Republic of ChinaTel/Fax +86 537 2106208Email jiangpeicsu@sina.comPurpose: Substantial evidence indicates that lipopolysaccharide (LPS) exposure can lead to systemic inflammatory response syndrome (SIRS) and multiple organ failure. Previous metabolomic studies have mainly focused on LPS-induced depression or hepatic and renal effects. However, no comprehensive metabolomics-based analysis of the serum, liver, kidney, hippocampus, and heart following exposure to LPS has been undertaken to date.Material and Methods: Male Sprague–Dawley rats were randomly allocated to a control and a LPS-treated group (n=8). LPS for 2 weeks (0.5 mg/kg every other day) was given via intraperitoneal injection. Gas chromatography–mass spectrometry (GC–MS) was used for metabolite determination, while multivariate statistical analysis was performed to identify differentially expressed metabolites between the two groups.Results: Our study revealed that 24, 13, 12, 7, and 12 metabolites were differentially expressed between the LPS treatment group and the control group in the serum, liver, kidney, hippocampus, and heart, respectively. We further identified that these metabolic changes were mainly involved with aminoacyl-tRNA biosynthesis; glutathione metabolism; glyoxylate and dicarboxylate metabolism; glycine, serine, and threonine metabolism; arginine biosynthesis; bile acid biosynthesis; and glycerolipid metabolism.Conclusion: We have systematically elucidated the metabolic changes underlying LPS-induced SIRS, thereby providing insight into the mechanisms associated with these alterations.Keywords: systemic inflammatory response syndrome, metabolites, gas chromatography–mass spectrometry, multivariate statistical analysis
format	article
author	Geng C Guo Y Wang C Cui C Han W Liao D Jiang P
author_facet	Geng C Guo Y Wang C Cui C Han W Liao D Jiang P
author_sort	Geng C
title	Comprehensive Evaluation of Lipopolysaccharide-Induced Changes in Rats Based on Metabolomics
title_short	Comprehensive Evaluation of Lipopolysaccharide-Induced Changes in Rats Based on Metabolomics
title_full	Comprehensive Evaluation of Lipopolysaccharide-Induced Changes in Rats Based on Metabolomics
title_fullStr	Comprehensive Evaluation of Lipopolysaccharide-Induced Changes in Rats Based on Metabolomics
title_full_unstemmed	Comprehensive Evaluation of Lipopolysaccharide-Induced Changes in Rats Based on Metabolomics
title_sort	comprehensive evaluation of lipopolysaccharide-induced changes in rats based on metabolomics
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/16037bfcc4b543be8bd6d670a5975800
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Comprehensive Evaluation of Lipopolysaccharide-Induced Changes in Rats Based on Metabolomics

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