Cenderitide-eluting film for potential cardiac patch applications.

Cenderitide, also known as CD-NP, is a designer peptide developed by combining native mammalian c-type natriuretic peptide (CNP) and the C-terminus isolated from the dendroapis natriuretic peptide (DNP) of the venom from the green mamba. In early studies, intravenous and subcutaneous infusion of cen...

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Autores principales:	Xu Wen Ng, Yingying Huang, Horng H Chen, John C Burnett, Freddy Y C Boey, Subbu S Venkatraman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1604ed4dd22a434e92171cd3e1a114f0
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spelling	oai:doaj.org-article:1604ed4dd22a434e92171cd3e1a114f02021-11-18T07:38:31ZCenderitide-eluting film for potential cardiac patch applications.1932-620310.1371/journal.pone.0068346https://doaj.org/article/1604ed4dd22a434e92171cd3e1a114f02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23861890/?tool=EBIhttps://doaj.org/toc/1932-6203Cenderitide, also known as CD-NP, is a designer peptide developed by combining native mammalian c-type natriuretic peptide (CNP) and the C-terminus isolated from the dendroapis natriuretic peptide (DNP) of the venom from the green mamba. In early studies, intravenous and subcutaneous infusion of cenderitide was reported to reduce left ventricular (LV) mass and ameliorate cardiac remodelling. In this work, biodegradable polymeric films encapsulating CD-NP were developed and were investigated for their in vitro release and degradation characteristics. Subsequently, the bioactivity of released peptide and its effects on human cardiac fibroblast (HCF) were explored. We achieved sustained release from three films with low, intermediate and high release profiles for 30 days. Moreover, the bioactivity of released peptide was verified from the elevated production of cyclic guanosine monophospate (cGMP). The CD-NP released from films was able to inhibit the proliferation of hypertrophic HCF as well as suppress DNA synthesis in HCF. Furthermore, the sustained delivery from films showed comparable or superior suppressive actions on hypertrophic HCF compared to daily infusion of CD-NP. The results suggest that these films could be used to inhibit fibrosis and reduce cardiac remodelling via local delivery as cardiac patches.Xu Wen NgYingying HuangHorng H ChenJohn C BurnettFreddy Y C BoeySubbu S VenkatramanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68346 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xu Wen Ng Yingying Huang Horng H Chen John C Burnett Freddy Y C Boey Subbu S Venkatraman Cenderitide-eluting film for potential cardiac patch applications.
description	Cenderitide, also known as CD-NP, is a designer peptide developed by combining native mammalian c-type natriuretic peptide (CNP) and the C-terminus isolated from the dendroapis natriuretic peptide (DNP) of the venom from the green mamba. In early studies, intravenous and subcutaneous infusion of cenderitide was reported to reduce left ventricular (LV) mass and ameliorate cardiac remodelling. In this work, biodegradable polymeric films encapsulating CD-NP were developed and were investigated for their in vitro release and degradation characteristics. Subsequently, the bioactivity of released peptide and its effects on human cardiac fibroblast (HCF) were explored. We achieved sustained release from three films with low, intermediate and high release profiles for 30 days. Moreover, the bioactivity of released peptide was verified from the elevated production of cyclic guanosine monophospate (cGMP). The CD-NP released from films was able to inhibit the proliferation of hypertrophic HCF as well as suppress DNA synthesis in HCF. Furthermore, the sustained delivery from films showed comparable or superior suppressive actions on hypertrophic HCF compared to daily infusion of CD-NP. The results suggest that these films could be used to inhibit fibrosis and reduce cardiac remodelling via local delivery as cardiac patches.
format	article
author	Xu Wen Ng Yingying Huang Horng H Chen John C Burnett Freddy Y C Boey Subbu S Venkatraman
author_facet	Xu Wen Ng Yingying Huang Horng H Chen John C Burnett Freddy Y C Boey Subbu S Venkatraman
author_sort	Xu Wen Ng
title	Cenderitide-eluting film for potential cardiac patch applications.
title_short	Cenderitide-eluting film for potential cardiac patch applications.
title_full	Cenderitide-eluting film for potential cardiac patch applications.
title_fullStr	Cenderitide-eluting film for potential cardiac patch applications.
title_full_unstemmed	Cenderitide-eluting film for potential cardiac patch applications.
title_sort	cenderitide-eluting film for potential cardiac patch applications.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1604ed4dd22a434e92171cd3e1a114f0
work_keys_str_mv	AT xuwenng cenderitideelutingfilmforpotentialcardiacpatchapplications AT yingyinghuang cenderitideelutingfilmforpotentialcardiacpatchapplications AT hornghchen cenderitideelutingfilmforpotentialcardiacpatchapplications AT johncburnett cenderitideelutingfilmforpotentialcardiacpatchapplications AT freddyycboey cenderitideelutingfilmforpotentialcardiacpatchapplications AT subbusvenkatraman cenderitideelutingfilmforpotentialcardiacpatchapplications
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Cenderitide-eluting film for potential cardiac patch applications.

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