Antitumor Effects of Self-Assembling Peptide-Emodin in situ Hydrogels in vitro and in vivo

Weipeng Wei,1– 3,* Jianhua Tang,4,* Hongfang Li,1– 3 Yongsheng Huang,5 Chengchen Yin,1– 3 Dan Li,6 Fushan Tang1– 3 1Department of Clinical Pharmacy, Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zuny...

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Autores principales:	Wei W, Tang J, Li H, Huang Y, Yin C, Li D, Tang F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	self-assembling peptide emodin in situ hydrogels antitumor drug delivery system Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/160988e2c2af4fcb93b12e08a544dbcd
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spelling	oai:doaj.org-article:160988e2c2af4fcb93b12e08a544dbcd2021-12-02T16:01:14ZAntitumor Effects of Self-Assembling Peptide-Emodin in situ Hydrogels in vitro and in vivo1178-2013https://doaj.org/article/160988e2c2af4fcb93b12e08a544dbcd2021-01-01T00:00:00Zhttps://www.dovepress.com/antitumor-effects-of-self-assembling-peptide-emodin-in-situ-hydrogels--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Weipeng Wei,1– 3,* Jianhua Tang,4,* Hongfang Li,1– 3 Yongsheng Huang,5 Chengchen Yin,1– 3 Dan Li,6 Fushan Tang1– 3 1Department of Clinical Pharmacy, Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi 563000, People’s Republic of China; 2Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, People’s Republic of China; 3Key Laboratory of Clinical Pharmacy of Zunyi City, Zunyi Medical University, Zunyi 563000, People’s Republic of China; 4Cancer Research UK Manchester Institute, The University of Manchester, Cheshire SK10 4TG, UK; 5Peking Union Medical College, Chinese Academy of Medical Sciences, Institute of Basic Medical Sciences, Beijing 100005, People’s Republic of China; 6State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fushan TangDepartment of Clinical Pharmacy, School of Pharmacy, Zunyi Medical University, Guizhou, People’s Republic of ChinaTel +86851 28642337Fax +86851 28642334Email fstang@vip.163.comDan LiCancer Hospital, Chinese Academy of Medical Sciences, Beijing, People’s Republic of ChinaTel +8610 87787107Email eileenld@gmail.comPurpose: To study the in vitro and in vivo antitumor effects of the colloidal suspension-in situ hydrogel of emodin (EM) constructed with the self-assembling peptide RADA16-I and systematically evaluate the feasibility of the delivery system.Methods: The MTT and colony-formation assays were used to determine the viability of normal cells NCTC 1469 and tumor cells Hepa1-6. The uptake of EM in the RADA16-I-EM in situ hydrogel by tumor cells was analyzed by laser confocal microscope and flow cytometry. Flow cytometry was used to detect the cell apoptosis and cell cycle distribution. Transwell assay was used to detect the migration and invasion of tumor cells. The antitumor efficacy of the RADA16-I-EM in situ hydrogel and its toxic effects was further assessed in vivo on Hepa1-6 tumor-bearing C57 mice.Results: The results showed that the RADA16-I-EM in situ hydrogels could obviously reduce the toxicity of EM to normal cells and the survival of tumor cells. The uptake of EM by the cells from the hydrogels was obviously increased and could significantly induce apoptosis and arrest cell cycle in the G2/M phase, and reduce the migration, invasion and clone-formation ability of the cells. The RADA16-I-EM in situ hydrogel could also effectively inhibit the tumor growth and obviously decrease the toxic effects of EM on normal tissues in vivo.Conclusion: Our results demonstrated that RADA16-I has the potential to be a carrier for the hydrophobic drug EM and can effectively improve the delivery of hydrophobic antitumor drugs with enhanced antitumor effects and reduced toxic effects of the drugs on normal cells and tissues.Keywords: self-assembling peptide, emodin, in situ hydrogels, antitumor, drug delivery systemWei WTang JLi HHuang YYin CLi DTang FDove Medical Pressarticleself-assembling peptideemodinin situ hydrogelsantitumordrug delivery systemMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 47-60 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	self-assembling peptide emodin in situ hydrogels antitumor drug delivery system Medicine (General) R5-920
spellingShingle	self-assembling peptide emodin in situ hydrogels antitumor drug delivery system Medicine (General) R5-920 Wei W Tang J Li H Huang Y Yin C Li D Tang F Antitumor Effects of Self-Assembling Peptide-Emodin in situ Hydrogels in vitro and in vivo
description	Weipeng Wei,1– 3,* Jianhua Tang,4,* Hongfang Li,1– 3 Yongsheng Huang,5 Chengchen Yin,1– 3 Dan Li,6 Fushan Tang1– 3 1Department of Clinical Pharmacy, Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi 563000, People’s Republic of China; 2Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, People’s Republic of China; 3Key Laboratory of Clinical Pharmacy of Zunyi City, Zunyi Medical University, Zunyi 563000, People’s Republic of China; 4Cancer Research UK Manchester Institute, The University of Manchester, Cheshire SK10 4TG, UK; 5Peking Union Medical College, Chinese Academy of Medical Sciences, Institute of Basic Medical Sciences, Beijing 100005, People’s Republic of China; 6State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fushan TangDepartment of Clinical Pharmacy, School of Pharmacy, Zunyi Medical University, Guizhou, People’s Republic of ChinaTel +86851 28642337Fax +86851 28642334Email fstang@vip.163.comDan LiCancer Hospital, Chinese Academy of Medical Sciences, Beijing, People’s Republic of ChinaTel +8610 87787107Email eileenld@gmail.comPurpose: To study the in vitro and in vivo antitumor effects of the colloidal suspension-in situ hydrogel of emodin (EM) constructed with the self-assembling peptide RADA16-I and systematically evaluate the feasibility of the delivery system.Methods: The MTT and colony-formation assays were used to determine the viability of normal cells NCTC 1469 and tumor cells Hepa1-6. The uptake of EM in the RADA16-I-EM in situ hydrogel by tumor cells was analyzed by laser confocal microscope and flow cytometry. Flow cytometry was used to detect the cell apoptosis and cell cycle distribution. Transwell assay was used to detect the migration and invasion of tumor cells. The antitumor efficacy of the RADA16-I-EM in situ hydrogel and its toxic effects was further assessed in vivo on Hepa1-6 tumor-bearing C57 mice.Results: The results showed that the RADA16-I-EM in situ hydrogels could obviously reduce the toxicity of EM to normal cells and the survival of tumor cells. The uptake of EM by the cells from the hydrogels was obviously increased and could significantly induce apoptosis and arrest cell cycle in the G2/M phase, and reduce the migration, invasion and clone-formation ability of the cells. The RADA16-I-EM in situ hydrogel could also effectively inhibit the tumor growth and obviously decrease the toxic effects of EM on normal tissues in vivo.Conclusion: Our results demonstrated that RADA16-I has the potential to be a carrier for the hydrophobic drug EM and can effectively improve the delivery of hydrophobic antitumor drugs with enhanced antitumor effects and reduced toxic effects of the drugs on normal cells and tissues.Keywords: self-assembling peptide, emodin, in situ hydrogels, antitumor, drug delivery system
format	article
author	Wei W Tang J Li H Huang Y Yin C Li D Tang F
author_facet	Wei W Tang J Li H Huang Y Yin C Li D Tang F
author_sort	Wei W
title	Antitumor Effects of Self-Assembling Peptide-Emodin in situ Hydrogels in vitro and in vivo
title_short	Antitumor Effects of Self-Assembling Peptide-Emodin in situ Hydrogels in vitro and in vivo
title_full	Antitumor Effects of Self-Assembling Peptide-Emodin in situ Hydrogels in vitro and in vivo
title_fullStr	Antitumor Effects of Self-Assembling Peptide-Emodin in situ Hydrogels in vitro and in vivo
title_full_unstemmed	Antitumor Effects of Self-Assembling Peptide-Emodin in situ Hydrogels in vitro and in vivo
title_sort	antitumor effects of self-assembling peptide-emodin in situ hydrogels in vitro and in vivo
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/160988e2c2af4fcb93b12e08a544dbcd
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Antitumor Effects of Self-Assembling Peptide-Emodin in situ Hydrogels in vitro and in vivo

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