Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis

Abstract Kashin-Beck disease (KBD) is a chronic osteochondropathy with unclear pathogeny. In this study, we compared the microRNA expression profiles of 16 KBD patients, 16 osteoarthritis (OA) patients and 16 rheumatoid arthritis (RA) patients and 16 healthy controls in their blood specimens. miRNAs...

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Autores principales:	Wenhong Wu, Awen He, Yan Wen, Xiao Xiao, Jingcan Hao, Feng Zhang, Xiong Guo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/160bfb476ea2456d88676ba03cc8fa00
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spelling	oai:doaj.org-article:160bfb476ea2456d88676ba03cc8fa002021-12-02T15:06:18ZComparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis10.1038/s41598-017-00522-z2045-2322https://doaj.org/article/160bfb476ea2456d88676ba03cc8fa002017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00522-zhttps://doaj.org/toc/2045-2322Abstract Kashin-Beck disease (KBD) is a chronic osteochondropathy with unclear pathogeny. In this study, we compared the microRNA expression profiles of 16 KBD patients, 16 osteoarthritis (OA) patients and 16 rheumatoid arthritis (RA) patients and 16 healthy controls in their blood specimens. miRNAs expression profiling was performed using Exiqon miRCURY LNATM miRNAs Array. miRNAs target genes were predicted using miRror suite. Another independent mRNA expression profile dataset of 20 KBD patients and 15 healthy controls were integrated with the miRNA expression profiles of KBD. We identified 140 differently expressed miRNAs in KBD vs. Controls. GO enrichment analysis found that hypoxia, Wnt receptor signaling pathway and vitamin B6 biosynthesis related GO terms were significantly overrepresented in the target genes of differently expressed miRNAs in KBD vs. Control. 18 differently expressed common miRNAs were identified in KBD vs. Control, KBD vs. OA and KBD vs. RA. Integrating the lists of differently expressed miRNA target genes and mRNA differently expressed genes detected 6 common genes for KBD. Our results demonstrated the altered miRNAs expression profiles of KBD comparing to healthy controls, OA and RA, which provide novel clues for clarifying the mechanism of KBD.Wenhong WuAwen HeYan WenXiao XiaoJingcan HaoFeng ZhangXiong GuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenhong Wu Awen He Yan Wen Xiao Xiao Jingcan Hao Feng Zhang Xiong Guo Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis
description	Abstract Kashin-Beck disease (KBD) is a chronic osteochondropathy with unclear pathogeny. In this study, we compared the microRNA expression profiles of 16 KBD patients, 16 osteoarthritis (OA) patients and 16 rheumatoid arthritis (RA) patients and 16 healthy controls in their blood specimens. miRNAs expression profiling was performed using Exiqon miRCURY LNATM miRNAs Array. miRNAs target genes were predicted using miRror suite. Another independent mRNA expression profile dataset of 20 KBD patients and 15 healthy controls were integrated with the miRNA expression profiles of KBD. We identified 140 differently expressed miRNAs in KBD vs. Controls. GO enrichment analysis found that hypoxia, Wnt receptor signaling pathway and vitamin B6 biosynthesis related GO terms were significantly overrepresented in the target genes of differently expressed miRNAs in KBD vs. Control. 18 differently expressed common miRNAs were identified in KBD vs. Control, KBD vs. OA and KBD vs. RA. Integrating the lists of differently expressed miRNA target genes and mRNA differently expressed genes detected 6 common genes for KBD. Our results demonstrated the altered miRNAs expression profiles of KBD comparing to healthy controls, OA and RA, which provide novel clues for clarifying the mechanism of KBD.
format	article
author	Wenhong Wu Awen He Yan Wen Xiao Xiao Jingcan Hao Feng Zhang Xiong Guo
author_facet	Wenhong Wu Awen He Yan Wen Xiao Xiao Jingcan Hao Feng Zhang Xiong Guo
author_sort	Wenhong Wu
title	Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis
title_short	Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis
title_full	Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis
title_fullStr	Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis
title_full_unstemmed	Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis
title_sort	comparison of microrna expression profiles of kashin-beck disease, osteoarthritis and rheumatoid arthritis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/160bfb476ea2456d88676ba03cc8fa00
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Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis

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