Paroxetine ameliorates changes in hippocampal energy metabolism in chronic mild stress-exposed rats

Lobna H Khedr, Noha N Nassar, Ezzeldin S El-Denshary, Ahmed M Abdel-tawab 1Department of Pharmacology, Faculty of Pharmacy, Misr International University, 2Department of Pharmacology, Faculty of Pharmacy, Cairo University, 3Department of Pharmacology, Faculty of Medicine, Ain Shams University, Cair...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Khedr LH, Nassar NN, El-Denshary ES, Abdel-tawab AM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/160d54eab0024e32be44f7c1464fd352
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:160d54eab0024e32be44f7c1464fd352
record_format	dspace
spelling	oai:doaj.org-article:160d54eab0024e32be44f7c1464fd3522021-12-02T08:36:07ZParoxetine ameliorates changes in hippocampal energy metabolism in chronic mild stress-exposed rats1178-2021https://doaj.org/article/160d54eab0024e32be44f7c1464fd3522015-11-01T00:00:00Zhttps://www.dovepress.com/paroxetine-ameliorates-changes-in-hippocampal-energy-metabolism-in-chr-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Lobna H Khedr, Noha N Nassar, Ezzeldin S El-Denshary, Ahmed M Abdel-tawab 1Department of Pharmacology, Faculty of Pharmacy, Misr International University, 2Department of Pharmacology, Faculty of Pharmacy, Cairo University, 3Department of Pharmacology, Faculty of Medicine, Ain Shams University, Cairo, Egypt Abstract: The molecular mechanisms underlying stress-induced depression have not been fully outlined. Hence, the current study aimed at testing the link between behavioral changes in chronic mild stress (CMS) model and changes in hippocampal energy metabolism and the role of paroxetine (PAROX) in ameliorating these changes. Male Wistar rats were divided into three groups: vehicle control, CMS-exposed rats, and CMS-exposed rats receiving PAROX (10 mg/kg/day intraperitoneally). Sucrose preference, open-field, and forced swimming tests were carried out. Corticosterone (CORT) was measured in serum, while adenosine triphosphate and its metabolites, cytosolic cytochrome-c (Cyt-c), caspase-3 (Casp-3), as well as nitric oxide metabolites (NOx) were measured in hippocampal tissue homogenates. CMS-exposed rats showed a decrease in sucrose preference as well as body weight compared to control, which was reversed by PAROX. The latter further ameliorated the CMS-induced elevation of CORT in serum (91.71±1.77 ng/mL vs 124.5±4.44 ng/mL, P<0.001) as well as the changes in adenosine triphosphate/adenosine diphosphate (3.76±0.02 nmol/mg protein vs 1.07±0.01 nmol/mg protein, P<0.001). Furthermore, PAROX reduced the expression of Cyt-c and Casp-3, as well as restoring NOx levels. This study highlights the role of PAROX in reversing depressive behavior associated with stress-induced apoptosis and changes in hippocampal energy metabolism in the CMS model of depression. Keywords: rats, CMS, hippocampus, paroxetine, apoptosis, adenine nucleotides, cytochrome-c, caspase-3Khedr LHNassar NNEl-Denshary ESAbdel-tawab AMDove Medical PressarticleNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol 2015, Iss default, Pp 2887-2901 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Khedr LH Nassar NN El-Denshary ES Abdel-tawab AM Paroxetine ameliorates changes in hippocampal energy metabolism in chronic mild stress-exposed rats
description	Lobna H Khedr, Noha N Nassar, Ezzeldin S El-Denshary, Ahmed M Abdel-tawab 1Department of Pharmacology, Faculty of Pharmacy, Misr International University, 2Department of Pharmacology, Faculty of Pharmacy, Cairo University, 3Department of Pharmacology, Faculty of Medicine, Ain Shams University, Cairo, Egypt Abstract: The molecular mechanisms underlying stress-induced depression have not been fully outlined. Hence, the current study aimed at testing the link between behavioral changes in chronic mild stress (CMS) model and changes in hippocampal energy metabolism and the role of paroxetine (PAROX) in ameliorating these changes. Male Wistar rats were divided into three groups: vehicle control, CMS-exposed rats, and CMS-exposed rats receiving PAROX (10 mg/kg/day intraperitoneally). Sucrose preference, open-field, and forced swimming tests were carried out. Corticosterone (CORT) was measured in serum, while adenosine triphosphate and its metabolites, cytosolic cytochrome-c (Cyt-c), caspase-3 (Casp-3), as well as nitric oxide metabolites (NOx) were measured in hippocampal tissue homogenates. CMS-exposed rats showed a decrease in sucrose preference as well as body weight compared to control, which was reversed by PAROX. The latter further ameliorated the CMS-induced elevation of CORT in serum (91.71±1.77 ng/mL vs 124.5±4.44 ng/mL, P<0.001) as well as the changes in adenosine triphosphate/adenosine diphosphate (3.76±0.02 nmol/mg protein vs 1.07±0.01 nmol/mg protein, P<0.001). Furthermore, PAROX reduced the expression of Cyt-c and Casp-3, as well as restoring NOx levels. This study highlights the role of PAROX in reversing depressive behavior associated with stress-induced apoptosis and changes in hippocampal energy metabolism in the CMS model of depression. Keywords: rats, CMS, hippocampus, paroxetine, apoptosis, adenine nucleotides, cytochrome-c, caspase-3
format	article
author	Khedr LH Nassar NN El-Denshary ES Abdel-tawab AM
author_facet	Khedr LH Nassar NN El-Denshary ES Abdel-tawab AM
author_sort	Khedr LH
title	Paroxetine ameliorates changes in hippocampal energy metabolism in chronic mild stress-exposed rats
title_short	Paroxetine ameliorates changes in hippocampal energy metabolism in chronic mild stress-exposed rats
title_full	Paroxetine ameliorates changes in hippocampal energy metabolism in chronic mild stress-exposed rats
title_fullStr	Paroxetine ameliorates changes in hippocampal energy metabolism in chronic mild stress-exposed rats
title_full_unstemmed	Paroxetine ameliorates changes in hippocampal energy metabolism in chronic mild stress-exposed rats
title_sort	paroxetine ameliorates changes in hippocampal energy metabolism in chronic mild stress-exposed rats
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/160d54eab0024e32be44f7c1464fd352
work_keys_str_mv	AT khedrlh paroxetineameliorateschangesinhippocampalenergymetabolisminchronicmildstressexposedrats AT nassarnn paroxetineameliorateschangesinhippocampalenergymetabolisminchronicmildstressexposedrats AT eldensharyes paroxetineameliorateschangesinhippocampalenergymetabolisminchronicmildstressexposedrats AT abdeltawabam paroxetineameliorateschangesinhippocampalenergymetabolisminchronicmildstressexposedrats
_version_	1718398447189491712

Paroxetine ameliorates changes in hippocampal energy metabolism in chronic mild stress-exposed rats

Ejemplares similares