Multiplex suppression of four quadruplet codons via tRNA directed evolution

Genetic code expansion strategies are limited to specific codons that can be reassigned to new amino acids. Here the authors show that quadruplet-decoding tRNAs (qtRNAs) can be rapidly discovered and evolved to decode new quadruplet codons, enabling four independent decoding events in a single prote...

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Autores principales:	Erika A. DeBenedictis, Gavriela D. Carver, Christina Z. Chung, Dieter Söll, Ahmed H. Badran
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/160d7d8ec84743218f1d2a710d9af0a0
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spelling	oai:doaj.org-article:160d7d8ec84743218f1d2a710d9af0a02021-12-02T17:37:41ZMultiplex suppression of four quadruplet codons via tRNA directed evolution10.1038/s41467-021-25948-y2041-1723https://doaj.org/article/160d7d8ec84743218f1d2a710d9af0a02021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25948-yhttps://doaj.org/toc/2041-1723Genetic code expansion strategies are limited to specific codons that can be reassigned to new amino acids. Here the authors show that quadruplet-decoding tRNAs (qtRNAs) can be rapidly discovered and evolved to decode new quadruplet codons, enabling four independent decoding events in a single protein in living cells.Erika A. DeBenedictisGavriela D. CarverChristina Z. ChungDieter SöllAhmed H. BadranNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Erika A. DeBenedictis Gavriela D. Carver Christina Z. Chung Dieter Söll Ahmed H. Badran Multiplex suppression of four quadruplet codons via tRNA directed evolution
description	Genetic code expansion strategies are limited to specific codons that can be reassigned to new amino acids. Here the authors show that quadruplet-decoding tRNAs (qtRNAs) can be rapidly discovered and evolved to decode new quadruplet codons, enabling four independent decoding events in a single protein in living cells.
format	article
author	Erika A. DeBenedictis Gavriela D. Carver Christina Z. Chung Dieter Söll Ahmed H. Badran
author_facet	Erika A. DeBenedictis Gavriela D. Carver Christina Z. Chung Dieter Söll Ahmed H. Badran
author_sort	Erika A. DeBenedictis
title	Multiplex suppression of four quadruplet codons via tRNA directed evolution
title_short	Multiplex suppression of four quadruplet codons via tRNA directed evolution
title_full	Multiplex suppression of four quadruplet codons via tRNA directed evolution
title_fullStr	Multiplex suppression of four quadruplet codons via tRNA directed evolution
title_full_unstemmed	Multiplex suppression of four quadruplet codons via tRNA directed evolution
title_sort	multiplex suppression of four quadruplet codons via trna directed evolution
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/160d7d8ec84743218f1d2a710d9af0a0
work_keys_str_mv	AT erikaadebenedictis multiplexsuppressionoffourquadrupletcodonsviatrnadirectedevolution AT gavrieladcarver multiplexsuppressionoffourquadrupletcodonsviatrnadirectedevolution AT christinazchung multiplexsuppressionoffourquadrupletcodonsviatrnadirectedevolution AT dietersoll multiplexsuppressionoffourquadrupletcodonsviatrnadirectedevolution AT ahmedhbadran multiplexsuppressionoffourquadrupletcodonsviatrnadirectedevolution
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Multiplex suppression of four quadruplet codons via tRNA directed evolution

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