Effects of pH-sensitive chain length on release of doxorubicin from mPEG-b-PH-b-PLLA nanoparticles

Rong Liu,1,2 Bin He,1 Dong Li,1 Yusi Lai,1 Jing Chang,1 James Z Tang,3 Zhongwei Gu11National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 2Dalian Institute of Chemical Physics Chinese Academy of Sciences, Dalian, China; 3Department of Pharmacy, School of Applied Science...

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Autores principales:	Liu R, He B, Li D, Lai Y, Chang J, Tang JZ, Gu Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/161411c945254840ad1f478465d5f13d
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spelling	oai:doaj.org-article:161411c945254840ad1f478465d5f13d2021-12-02T00:24:26ZEffects of pH-sensitive chain length on release of doxorubicin from mPEG-b-PH-b-PLLA nanoparticles1176-91141178-2013https://doaj.org/article/161411c945254840ad1f478465d5f13d2012-08-01T00:00:00Zhttp://www.dovepress.com/effects-of-ph-sensitive-chain-length-on-release-of-doxorubicin-from-mp-a10690https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Rong Liu,1,2 Bin He,1 Dong Li,1 Yusi Lai,1 Jing Chang,1 James Z Tang,3 Zhongwei Gu11National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 2Dalian Institute of Chemical Physics Chinese Academy of Sciences, Dalian, China; 3Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, Wolverhampton, United KingdomBackground: Two methoxyl poly(ethylene glycol)-poly(L-histidine)-poly(L-lactide) (mPEG-PH-PLLA) triblock copolymers with different poly(L-histidine) chain lengths were synthesized. The morphology and biocompatibility of these self-assembled nanoparticles was investigated.Methods: Doxorubicin, an antitumor drug, was trapped in the nanoparticles to explore their drug-release behavior. The drug-loaded nanoparticles were incubated with HepG2 cells to evaluate their antitumor efficacy in vitro. The effects of poly(L-histidine) chain length on the properties, drug-release behavior, and antitumor efficiency of the nanoparticles were investigated.Results: The nanoparticles were pH-sensitive. The mean diameters of the two types of mPEG-PH-PLLA nanoparticle were less than 200 nm when the pH values were 5.0 and 7.4. The nanoparticles were nontoxic to NIH 3T3 fibroblasts and HepG2 cells. The release of doxorubicin at pH 5.0 was much faster than that at pH 7.4. The release rate of mPEG45-PH15-PLLA82 nanoparticles was much faster than that of mPEG45-PH30-PLLA82 nanoparticles at pH 5.0.Conclusion: The inhibition effect of mPEG45-PH15-PLLA82 nanoparticles on the growth of HepG2 cells was greater than that of mPEG45-PH30-PLLA82 nanoparticles when the concentration of encapsulated doxorubicin was less than 15 &micro;g/mL.Keywords: poly(ethylene glycol), poly(L-histidine), poly(L-lactide), pH sensitivity, doxorubicin, drug release, nanoparticleLiu RHe BLi DLai YChang JTang JZGu ZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4433-4446 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Liu R He B Li D Lai Y Chang J Tang JZ Gu Z Effects of pH-sensitive chain length on release of doxorubicin from mPEG-b-PH-b-PLLA nanoparticles
description	Rong Liu,1,2 Bin He,1 Dong Li,1 Yusi Lai,1 Jing Chang,1 James Z Tang,3 Zhongwei Gu11National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 2Dalian Institute of Chemical Physics Chinese Academy of Sciences, Dalian, China; 3Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, Wolverhampton, United KingdomBackground: Two methoxyl poly(ethylene glycol)-poly(L-histidine)-poly(L-lactide) (mPEG-PH-PLLA) triblock copolymers with different poly(L-histidine) chain lengths were synthesized. The morphology and biocompatibility of these self-assembled nanoparticles was investigated.Methods: Doxorubicin, an antitumor drug, was trapped in the nanoparticles to explore their drug-release behavior. The drug-loaded nanoparticles were incubated with HepG2 cells to evaluate their antitumor efficacy in vitro. The effects of poly(L-histidine) chain length on the properties, drug-release behavior, and antitumor efficiency of the nanoparticles were investigated.Results: The nanoparticles were pH-sensitive. The mean diameters of the two types of mPEG-PH-PLLA nanoparticle were less than 200 nm when the pH values were 5.0 and 7.4. The nanoparticles were nontoxic to NIH 3T3 fibroblasts and HepG2 cells. The release of doxorubicin at pH 5.0 was much faster than that at pH 7.4. The release rate of mPEG45-PH15-PLLA82 nanoparticles was much faster than that of mPEG45-PH30-PLLA82 nanoparticles at pH 5.0.Conclusion: The inhibition effect of mPEG45-PH15-PLLA82 nanoparticles on the growth of HepG2 cells was greater than that of mPEG45-PH30-PLLA82 nanoparticles when the concentration of encapsulated doxorubicin was less than 15 &micro;g/mL.Keywords: poly(ethylene glycol), poly(L-histidine), poly(L-lactide), pH sensitivity, doxorubicin, drug release, nanoparticle
format	article
author	Liu R He B Li D Lai Y Chang J Tang JZ Gu Z
author_facet	Liu R He B Li D Lai Y Chang J Tang JZ Gu Z
author_sort	Liu R
title	Effects of pH-sensitive chain length on release of doxorubicin from mPEG-b-PH-b-PLLA nanoparticles
title_short	Effects of pH-sensitive chain length on release of doxorubicin from mPEG-b-PH-b-PLLA nanoparticles
title_full	Effects of pH-sensitive chain length on release of doxorubicin from mPEG-b-PH-b-PLLA nanoparticles
title_fullStr	Effects of pH-sensitive chain length on release of doxorubicin from mPEG-b-PH-b-PLLA nanoparticles
title_full_unstemmed	Effects of pH-sensitive chain length on release of doxorubicin from mPEG-b-PH-b-PLLA nanoparticles
title_sort	effects of ph-sensitive chain length on release of doxorubicin from mpeg-b-ph-b-plla nanoparticles
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/161411c945254840ad1f478465d5f13d
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Effects of pH-sensitive chain length on release of doxorubicin from mPEG-b-PH-b-PLLA nanoparticles

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