“High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”

Abstract The tissue-specific etiology of aging and stress has been elusive due to limitations in data processing of current techniques. Despite that many techniques are high-throughput, they usually use singular features of the data (e.g. whole fluorescence). One technology at the nexus of fluoresce...

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Autores principales:	Joseph R. Daniele, Daniel J. Esping, Gilbert Garcia, Lee S. Parsons, Edgar A. Arriaga, Andrew Dillin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/161665a6154340f8869de5212ae2387f
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spelling	oai:doaj.org-article:161665a6154340f8869de5212ae2387f2021-12-02T11:52:15Z“High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”10.1038/s41598-017-05152-z2045-2322https://doaj.org/article/161665a6154340f8869de5212ae2387f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05152-zhttps://doaj.org/toc/2045-2322Abstract The tissue-specific etiology of aging and stress has been elusive due to limitations in data processing of current techniques. Despite that many techniques are high-throughput, they usually use singular features of the data (e.g. whole fluorescence). One technology at the nexus of fluorescence-based screens is large particle flow cytometry (“biosorter”), capable of recording positional fluorescence and object granularity information from many individual live animals. Current processing of biosorter data, however, do not integrate positional information into their analysis and data visualization. Here, we present a bioanalytical platform for the quantification of positional information (“longitudinal profiling”) of C. elegans, which we posit embodies the benefits of both high-throughput screening and high-resolution microscopy. We show the use of these techniques in (1) characterizing distinct responses of a transcriptional reporter to various stresses in defined anatomical regions, (2) identifying regions of high mitochondrial membrane potential in live animals, (3) monitoring regional mitochondrial activity in aging models and during development, and (4) screening for regulators of muscle mitochondrial dynamics in a high-throughput format. This platform offers a significant improvement in the quality of high-throughput biosorter data analysis and visualization, opening new options for region-specific phenotypic screening of complex physiological phenomena and mitochondrial biology.Joseph R. DanieleDaniel J. EspingGilbert GarciaLee S. ParsonsEdgar A. ArriagaAndrew DillinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joseph R. Daniele Daniel J. Esping Gilbert Garcia Lee S. Parsons Edgar A. Arriaga Andrew Dillin “High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”
description	Abstract The tissue-specific etiology of aging and stress has been elusive due to limitations in data processing of current techniques. Despite that many techniques are high-throughput, they usually use singular features of the data (e.g. whole fluorescence). One technology at the nexus of fluorescence-based screens is large particle flow cytometry (“biosorter”), capable of recording positional fluorescence and object granularity information from many individual live animals. Current processing of biosorter data, however, do not integrate positional information into their analysis and data visualization. Here, we present a bioanalytical platform for the quantification of positional information (“longitudinal profiling”) of C. elegans, which we posit embodies the benefits of both high-throughput screening and high-resolution microscopy. We show the use of these techniques in (1) characterizing distinct responses of a transcriptional reporter to various stresses in defined anatomical regions, (2) identifying regions of high mitochondrial membrane potential in live animals, (3) monitoring regional mitochondrial activity in aging models and during development, and (4) screening for regulators of muscle mitochondrial dynamics in a high-throughput format. This platform offers a significant improvement in the quality of high-throughput biosorter data analysis and visualization, opening new options for region-specific phenotypic screening of complex physiological phenomena and mitochondrial biology.
format	article
author	Joseph R. Daniele Daniel J. Esping Gilbert Garcia Lee S. Parsons Edgar A. Arriaga Andrew Dillin
author_facet	Joseph R. Daniele Daniel J. Esping Gilbert Garcia Lee S. Parsons Edgar A. Arriaga Andrew Dillin
author_sort	Joseph R. Daniele
title	“High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”
title_short	“High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”
title_full	“High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”
title_fullStr	“High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”
title_full_unstemmed	“High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”
title_sort	“high-throughput characterization of region-specific mitochondrial function and morphology”
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/161665a6154340f8869de5212ae2387f
work_keys_str_mv	AT josephrdaniele highthroughputcharacterizationofregionspecificmitochondrialfunctionandmorphology AT danieljesping highthroughputcharacterizationofregionspecificmitochondrialfunctionandmorphology AT gilbertgarcia highthroughputcharacterizationofregionspecificmitochondrialfunctionandmorphology AT leesparsons highthroughputcharacterizationofregionspecificmitochondrialfunctionandmorphology AT edgaraarriaga highthroughputcharacterizationofregionspecificmitochondrialfunctionandmorphology AT andrewdillin highthroughputcharacterizationofregionspecificmitochondrialfunctionandmorphology
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“High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”

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