In vivo Pooled Screening: A Scalable Tool to Study the Complexity of Aging and Age-Related Disease

Biological aging, and the diseases of aging, occur in a complex in vivo environment, driven by multiple interacting processes. A convergence of recently developed technologies has enabled in vivo pooled screening: direct administration of a library of different perturbations to a living animal, with...

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Autores principales:	Martin Borch Jensen, Adam Marblestone
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	in vivo pooled screening direct in vivo screening aging models animal models gene therapy Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/1617cdbf4b7e4ed18ecba563c5fc813d
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spelling	oai:doaj.org-article:1617cdbf4b7e4ed18ecba563c5fc813d2021-12-01T20:38:35ZIn vivo Pooled Screening: A Scalable Tool to Study the Complexity of Aging and Age-Related Disease2673-621710.3389/fragi.2021.714926https://doaj.org/article/1617cdbf4b7e4ed18ecba563c5fc813d2021-08-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fragi.2021.714926/fullhttps://doaj.org/toc/2673-6217Biological aging, and the diseases of aging, occur in a complex in vivo environment, driven by multiple interacting processes. A convergence of recently developed technologies has enabled in vivo pooled screening: direct administration of a library of different perturbations to a living animal, with a subsequent readout that distinguishes the identity of each perturbation and its effect on individual cells within the animal. Such screens hold promise for efficiently applying functional genomics to aging processes in the full richness of the in vivo setting. In this review, we describe the technologies behind in vivo pooled screening, including a range of options for delivery, perturbation and readout methods, and outline their potential application to aging and age-related disease. We then suggest how in vivo pooled screening, together with emerging innovations in each of its technological underpinnings, could be extended to shed light on key open questions in aging biology, including the mechanisms and limits of epigenetic reprogramming and identifying cellular mediators of systemic signals in aging.Martin Borch JensenAdam MarblestoneAdam MarblestoneFrontiers Media S.A.articlein vivopooled screeningdirect in vivo screeningaging modelsanimal modelsgene therapyGeriatricsRC952-954.6ENFrontiers in Aging, Vol 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	in vivo pooled screening direct in vivo screening aging models animal models gene therapy Geriatrics RC952-954.6
spellingShingle	in vivo pooled screening direct in vivo screening aging models animal models gene therapy Geriatrics RC952-954.6 Martin Borch Jensen Adam Marblestone Adam Marblestone In vivo Pooled Screening: A Scalable Tool to Study the Complexity of Aging and Age-Related Disease
description	Biological aging, and the diseases of aging, occur in a complex in vivo environment, driven by multiple interacting processes. A convergence of recently developed technologies has enabled in vivo pooled screening: direct administration of a library of different perturbations to a living animal, with a subsequent readout that distinguishes the identity of each perturbation and its effect on individual cells within the animal. Such screens hold promise for efficiently applying functional genomics to aging processes in the full richness of the in vivo setting. In this review, we describe the technologies behind in vivo pooled screening, including a range of options for delivery, perturbation and readout methods, and outline their potential application to aging and age-related disease. We then suggest how in vivo pooled screening, together with emerging innovations in each of its technological underpinnings, could be extended to shed light on key open questions in aging biology, including the mechanisms and limits of epigenetic reprogramming and identifying cellular mediators of systemic signals in aging.
format	article
author	Martin Borch Jensen Adam Marblestone Adam Marblestone
author_facet	Martin Borch Jensen Adam Marblestone Adam Marblestone
author_sort	Martin Borch Jensen
title	In vivo Pooled Screening: A Scalable Tool to Study the Complexity of Aging and Age-Related Disease
title_short	In vivo Pooled Screening: A Scalable Tool to Study the Complexity of Aging and Age-Related Disease
title_full	In vivo Pooled Screening: A Scalable Tool to Study the Complexity of Aging and Age-Related Disease
title_fullStr	In vivo Pooled Screening: A Scalable Tool to Study the Complexity of Aging and Age-Related Disease
title_full_unstemmed	In vivo Pooled Screening: A Scalable Tool to Study the Complexity of Aging and Age-Related Disease
title_sort	in vivo pooled screening: a scalable tool to study the complexity of aging and age-related disease
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1617cdbf4b7e4ed18ecba563c5fc813d
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In vivo Pooled Screening: A Scalable Tool to Study the Complexity of Aging and Age-Related Disease

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