Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear

Abstract Efforts to contain the spread of chronic wasting disease (CWD), a fatal, contagious prion disease of cervids, would be aided by the availability of additional diagnostic tools. RT-QuIC assays allow ultrasensitive detection of prion seeds in a wide variety of cervid tissues, fluids and excre...

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Autores principales:	Natalia C. Ferreira, Jorge M. Charco, Jakob Plagenz, Christina D. Orru, Nathanial D. Denkers, Michael A. Metrick, Andrew G. Hughson, Karen A. Griffin, Brent Race, Edward A. Hoover, Joaquín Castilla, Tracy A. Nichols, Michael W. Miller, Byron Caughey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/161a06b194b3492ea12e6770ee888250
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spelling	oai:doaj.org-article:161a06b194b3492ea12e6770ee8882502021-12-02T14:37:39ZDetection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear10.1038/s41598-021-87295-82045-2322https://doaj.org/article/161a06b194b3492ea12e6770ee8882502021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87295-8https://doaj.org/toc/2045-2322Abstract Efforts to contain the spread of chronic wasting disease (CWD), a fatal, contagious prion disease of cervids, would be aided by the availability of additional diagnostic tools. RT-QuIC assays allow ultrasensitive detection of prion seeds in a wide variety of cervid tissues, fluids and excreta. The best documented antemortem diagnostic test involving RT-QuIC analysis targets lymphoid tissue in rectal biopsies. Here we have tested a more easily accessed specimen, ear pinna punches, using an improved RT-QuIC assay involving iron oxide magnetic extraction to detect CWD infections in asymptomatic mule and white-tailed deer. Comparison of multiple parts of the ear pinna indicated that a central punch spanning the auricular nerve provided the most consistent detection of CWD infection. When compared to results obtained from gold-standard retropharyngeal lymph node specimens, our RT-QuIC analyses of ear samples provided apparent diagnostic sensitivity (81%) and specificity (91%) that rivaled, or improved upon, those observed in previous analyses of rectal biopsies using RT-QuIC. These results provide evidence that RT-QuIC analysis of ear pinna punches may be a useful approach to detecting CWD infections in cervids.Natalia C. FerreiraJorge M. CharcoJakob PlagenzChristina D. OrruNathanial D. DenkersMichael A. MetrickAndrew G. HughsonKaren A. GriffinBrent RaceEdward A. HooverJoaquín CastillaTracy A. NicholsMichael W. MillerByron CaugheyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natalia C. Ferreira Jorge M. Charco Jakob Plagenz Christina D. Orru Nathanial D. Denkers Michael A. Metrick Andrew G. Hughson Karen A. Griffin Brent Race Edward A. Hoover Joaquín Castilla Tracy A. Nichols Michael W. Miller Byron Caughey Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear
description	Abstract Efforts to contain the spread of chronic wasting disease (CWD), a fatal, contagious prion disease of cervids, would be aided by the availability of additional diagnostic tools. RT-QuIC assays allow ultrasensitive detection of prion seeds in a wide variety of cervid tissues, fluids and excreta. The best documented antemortem diagnostic test involving RT-QuIC analysis targets lymphoid tissue in rectal biopsies. Here we have tested a more easily accessed specimen, ear pinna punches, using an improved RT-QuIC assay involving iron oxide magnetic extraction to detect CWD infections in asymptomatic mule and white-tailed deer. Comparison of multiple parts of the ear pinna indicated that a central punch spanning the auricular nerve provided the most consistent detection of CWD infection. When compared to results obtained from gold-standard retropharyngeal lymph node specimens, our RT-QuIC analyses of ear samples provided apparent diagnostic sensitivity (81%) and specificity (91%) that rivaled, or improved upon, those observed in previous analyses of rectal biopsies using RT-QuIC. These results provide evidence that RT-QuIC analysis of ear pinna punches may be a useful approach to detecting CWD infections in cervids.
format	article
author	Natalia C. Ferreira Jorge M. Charco Jakob Plagenz Christina D. Orru Nathanial D. Denkers Michael A. Metrick Andrew G. Hughson Karen A. Griffin Brent Race Edward A. Hoover Joaquín Castilla Tracy A. Nichols Michael W. Miller Byron Caughey
author_facet	Natalia C. Ferreira Jorge M. Charco Jakob Plagenz Christina D. Orru Nathanial D. Denkers Michael A. Metrick Andrew G. Hughson Karen A. Griffin Brent Race Edward A. Hoover Joaquín Castilla Tracy A. Nichols Michael W. Miller Byron Caughey
author_sort	Natalia C. Ferreira
title	Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear
title_short	Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear
title_full	Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear
title_fullStr	Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear
title_full_unstemmed	Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear
title_sort	detection of chronic wasting disease in mule and white-tailed deer by rt-quic analysis of outer ear
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/161a06b194b3492ea12e6770ee888250
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Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear

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