Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold

Abstract Islets organoids would have value in the cell replacement therapy for diabetes apart from usual personalized drug screening routes. Generation of a large number of Islets like clusters, with ability to respond to glucose stimulation appears to be an ideal choice. In this study we have gener...

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Autores principales:	Shruti Sandilya, Shashi Singh
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Endocrine Exocrine Marker genes Crosslinked collagen Functional Insulin release Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/161cb5451b9f47ca811fee02eda16ae4
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spelling	oai:doaj.org-article:161cb5451b9f47ca811fee02eda16ae42021-12-05T12:24:39ZDevelopment of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold10.1186/s13619-021-00099-z2045-9769https://doaj.org/article/161cb5451b9f47ca811fee02eda16ae42021-12-01T00:00:00Zhttps://doi.org/10.1186/s13619-021-00099-zhttps://doaj.org/toc/2045-9769Abstract Islets organoids would have value in the cell replacement therapy for diabetes apart from usual personalized drug screening routes. Generation of a large number of Islets like clusters, with ability to respond to glucose stimulation appears to be an ideal choice. In this study we have generated islet organoids with the ability to respond to glucose stimulation by insulin release. The source of the cells was an iPSC cell line differentiated into the pancreatic progenitors. These cells were assembled in matrigel or cross-linked collagen scaffold and compared for their efficacy to release insulin upon stimulation with glucose. The assembled organoids were examined by immunohistochemistry and expression of the relevant marker genes. The organoids showed expression of islet like markers in both - matrigel and crosslinked collagen scaffold. The islet organoids in both the cases showed release of insulin upon stimulation with glucose. The crosslinked collagen scaffold is quite stable and supports islet cells growth and function.Shruti SandilyaShashi SinghSpringerOpenarticleEndocrineExocrineMarker genesCrosslinked collagenFunctionalInsulin releaseMedicine (General)R5-920Biology (General)QH301-705.5ENCell Regeneration, Vol 10, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Endocrine Exocrine Marker genes Crosslinked collagen Functional Insulin release Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	Endocrine Exocrine Marker genes Crosslinked collagen Functional Insulin release Medicine (General) R5-920 Biology (General) QH301-705.5 Shruti Sandilya Shashi Singh Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold
description	Abstract Islets organoids would have value in the cell replacement therapy for diabetes apart from usual personalized drug screening routes. Generation of a large number of Islets like clusters, with ability to respond to glucose stimulation appears to be an ideal choice. In this study we have generated islet organoids with the ability to respond to glucose stimulation by insulin release. The source of the cells was an iPSC cell line differentiated into the pancreatic progenitors. These cells were assembled in matrigel or cross-linked collagen scaffold and compared for their efficacy to release insulin upon stimulation with glucose. The assembled organoids were examined by immunohistochemistry and expression of the relevant marker genes. The organoids showed expression of islet like markers in both - matrigel and crosslinked collagen scaffold. The islet organoids in both the cases showed release of insulin upon stimulation with glucose. The crosslinked collagen scaffold is quite stable and supports islet cells growth and function.
format	article
author	Shruti Sandilya Shashi Singh
author_facet	Shruti Sandilya Shashi Singh
author_sort	Shruti Sandilya
title	Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold
title_short	Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold
title_full	Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold
title_fullStr	Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold
title_full_unstemmed	Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold
title_sort	development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/161cb5451b9f47ca811fee02eda16ae4
work_keys_str_mv	AT shrutisandilya developmentofisletorganoidsfromhumaninducedpluripotentstemcellsinacrosslinkedcollagenscaffold AT shashisingh developmentofisletorganoidsfromhumaninducedpluripotentstemcellsinacrosslinkedcollagenscaffold
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Development of islet organoids from human induced pluripotent stem cells in a cross-linked collagen scaffold

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