Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment

Abstract Human corneal endothelial cells (HCEnCs) are responsible for maintaining the transparency of the cornea. Damaged or diseased HCEnCs may cause blindness. Replacement of the diseased cells with a healthy donor endothelium is the only currently available treatment. Tissue-engineering can serve...

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Autores principales:	Mohit Parekh, Sajjad Ahmad, Alessandro Ruzza, Stefano Ferrari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/161fd002e7c74841af922f6611305ba6
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spelling	oai:doaj.org-article:161fd002e7c74841af922f6611305ba62021-12-02T12:30:36ZHuman Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment10.1038/s41598-017-00209-52045-2322https://doaj.org/article/161fd002e7c74841af922f6611305ba62017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00209-5https://doaj.org/toc/2045-2322Abstract Human corneal endothelial cells (HCEnCs) are responsible for maintaining the transparency of the cornea. Damaged or diseased HCEnCs may cause blindness. Replacement of the diseased cells with a healthy donor endothelium is the only currently available treatment. Tissue-engineering can serve as an alternative to conventional donor corneal transplantation. Due to the global shortage of donor corneas, a wide interest in the development of cultured graft substitutes and artificial corneas has increased. Availability of the old donor corneas is higher especially for research. Although it can be proposed as a valuable source for cell culture, its less proliferative capability emerges a challenge for the researchers. This article describes the use of hyaluronic acid (HA) in combination with Rho-kinase inhibitor (ROCK) Y-27632 for the cultivation of HCEnCs from older donor corneas (age > 60 years). Four conditions including and excluding HA + ROCK and its effect on early attachment rates and proliferation was studied on forty-eight corneas. It was observed that HCEnCs reach confluence within 10–15 days when cultured with HA + ROCK. This approach improves the efficiency of cell adhesion due to force attachment. HCEnCs from old donor corneas can be cultured using this method which may further lead to cell-based therapy for treating corneal endothelial dysfunction.Mohit ParekhSajjad AhmadAlessandro RuzzaStefano FerrariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohit Parekh Sajjad Ahmad Alessandro Ruzza Stefano Ferrari Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment
description	Abstract Human corneal endothelial cells (HCEnCs) are responsible for maintaining the transparency of the cornea. Damaged or diseased HCEnCs may cause blindness. Replacement of the diseased cells with a healthy donor endothelium is the only currently available treatment. Tissue-engineering can serve as an alternative to conventional donor corneal transplantation. Due to the global shortage of donor corneas, a wide interest in the development of cultured graft substitutes and artificial corneas has increased. Availability of the old donor corneas is higher especially for research. Although it can be proposed as a valuable source for cell culture, its less proliferative capability emerges a challenge for the researchers. This article describes the use of hyaluronic acid (HA) in combination with Rho-kinase inhibitor (ROCK) Y-27632 for the cultivation of HCEnCs from older donor corneas (age > 60 years). Four conditions including and excluding HA + ROCK and its effect on early attachment rates and proliferation was studied on forty-eight corneas. It was observed that HCEnCs reach confluence within 10–15 days when cultured with HA + ROCK. This approach improves the efficiency of cell adhesion due to force attachment. HCEnCs from old donor corneas can be cultured using this method which may further lead to cell-based therapy for treating corneal endothelial dysfunction.
format	article
author	Mohit Parekh Sajjad Ahmad Alessandro Ruzza Stefano Ferrari
author_facet	Mohit Parekh Sajjad Ahmad Alessandro Ruzza Stefano Ferrari
author_sort	Mohit Parekh
title	Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment
title_short	Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment
title_full	Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment
title_fullStr	Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment
title_full_unstemmed	Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment
title_sort	human corneal endothelial cell cultivation from old donor corneas with forced attachment
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/161fd002e7c74841af922f6611305ba6
work_keys_str_mv	AT mohitparekh humancornealendothelialcellcultivationfromolddonorcorneaswithforcedattachment AT sajjadahmad humancornealendothelialcellcultivationfromolddonorcorneaswithforcedattachment AT alessandroruzza humancornealendothelialcellcultivationfromolddonorcorneaswithforcedattachment AT stefanoferrari humancornealendothelialcellcultivationfromolddonorcorneaswithforcedattachment
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Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment

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