Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures
Visualising certain organelles and their dynamics is challenging in living cells. Here the authors co-opt selective degradation to label membrane-bound compartments in worm embryos and mammalian cells, revealing membrane topology during cell division.
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Nature Portfolio
2019
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oai:doaj.org-article:1625e4b9b53c47428c5ed687e5047f732021-12-02T16:57:55ZDegron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures10.1038/s41467-019-11442-z2041-1723https://doaj.org/article/1625e4b9b53c47428c5ed687e5047f732019-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11442-zhttps://doaj.org/toc/2041-1723Visualising certain organelles and their dynamics is challenging in living cells. Here the authors co-opt selective degradation to label membrane-bound compartments in worm embryos and mammalian cells, revealing membrane topology during cell division.Katharina B. BeerGholamreza FazeliKristyna JudasovaLinda IrmischJona CausemannJörg MansfeldAnn M. WehmanNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-16 (2019) |
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Science Q Katharina B. Beer Gholamreza Fazeli Kristyna Judasova Linda Irmisch Jona Causemann Jörg Mansfeld Ann M. Wehman Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures |
description |
Visualising certain organelles and their dynamics is challenging in living cells. Here the authors co-opt selective degradation to label membrane-bound compartments in worm embryos and mammalian cells, revealing membrane topology during cell division. |
format |
article |
author |
Katharina B. Beer Gholamreza Fazeli Kristyna Judasova Linda Irmisch Jona Causemann Jörg Mansfeld Ann M. Wehman |
author_facet |
Katharina B. Beer Gholamreza Fazeli Kristyna Judasova Linda Irmisch Jona Causemann Jörg Mansfeld Ann M. Wehman |
author_sort |
Katharina B. Beer |
title |
Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures |
title_short |
Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures |
title_full |
Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures |
title_fullStr |
Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures |
title_full_unstemmed |
Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures |
title_sort |
degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures |
publisher |
Nature Portfolio |
publishDate |
2019 |
url |
https://doaj.org/article/1625e4b9b53c47428c5ed687e5047f73 |
work_keys_str_mv |
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_version_ |
1718382430600036352 |