Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast

Abstract Cell polarity and morphogenesis are regulated by the small GTPase Cdc42. Even though major advances have been done in the field during the last years, the molecular details leading to its activation in particular cellular contexts are not completely understood. In fission yeast, the β(1,3)-...

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Autores principales:	Patricia García, Pedro M. Coll, Francisco del Rey, M. Isabel Geli, Pilar Pérez, Carlos R. Vázquez de Aldana, Javier Encinar del Dedo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16260262ad454e01a138c3a4d10f29e0
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spelling	oai:doaj.org-article:16260262ad454e01a138c3a4d10f29e02021-12-02T17:19:15ZEng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast10.1038/s41598-021-97311-62045-2322https://doaj.org/article/16260262ad454e01a138c3a4d10f29e02021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97311-6https://doaj.org/toc/2045-2322Abstract Cell polarity and morphogenesis are regulated by the small GTPase Cdc42. Even though major advances have been done in the field during the last years, the molecular details leading to its activation in particular cellular contexts are not completely understood. In fission yeast, the β(1,3)-glucanase Eng2 is a “moonlighting protein” with a dual function, acting as a hydrolase during spore dehiscence, and as component of the endocytic machinery in vegetative cells. Here, we report that Eng2 plays a role in Cdc42 activation during polarized growth through its interaction with the scaffold protein Scd2, which brings Cdc42 together with its guanine nucleotide exchange factor (GEF) Scd1. eng2Δ mutant cells have defects in activation of the bipolar growth (NETO), remaining monopolar during all the cell cycle. In the absence of Eng2 the accumulation of Scd1 and Scd2 at the poles is reduced, the levels of Cdc42 activation decrease, and the Cdc42 oscillatory behavior, associated with bipolar growth in wild type cells, is altered. Furthermore, overexpression of Eng2 partially rescues the growth and polarity defects of a cdc42-L160S mutant. Altogether, our work unveils a new factor regulating the activity of Cdc42, which could potentially link the polarity and endocytic machineries.Patricia GarcíaPedro M. CollFrancisco del ReyM. Isabel GeliPilar PérezCarlos R. Vázquez de AldanaJavier Encinar del DedoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patricia García Pedro M. Coll Francisco del Rey M. Isabel Geli Pilar Pérez Carlos R. Vázquez de Aldana Javier Encinar del Dedo Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast
description	Abstract Cell polarity and morphogenesis are regulated by the small GTPase Cdc42. Even though major advances have been done in the field during the last years, the molecular details leading to its activation in particular cellular contexts are not completely understood. In fission yeast, the β(1,3)-glucanase Eng2 is a “moonlighting protein” with a dual function, acting as a hydrolase during spore dehiscence, and as component of the endocytic machinery in vegetative cells. Here, we report that Eng2 plays a role in Cdc42 activation during polarized growth through its interaction with the scaffold protein Scd2, which brings Cdc42 together with its guanine nucleotide exchange factor (GEF) Scd1. eng2Δ mutant cells have defects in activation of the bipolar growth (NETO), remaining monopolar during all the cell cycle. In the absence of Eng2 the accumulation of Scd1 and Scd2 at the poles is reduced, the levels of Cdc42 activation decrease, and the Cdc42 oscillatory behavior, associated with bipolar growth in wild type cells, is altered. Furthermore, overexpression of Eng2 partially rescues the growth and polarity defects of a cdc42-L160S mutant. Altogether, our work unveils a new factor regulating the activity of Cdc42, which could potentially link the polarity and endocytic machineries.
format	article
author	Patricia García Pedro M. Coll Francisco del Rey M. Isabel Geli Pilar Pérez Carlos R. Vázquez de Aldana Javier Encinar del Dedo
author_facet	Patricia García Pedro M. Coll Francisco del Rey M. Isabel Geli Pilar Pérez Carlos R. Vázquez de Aldana Javier Encinar del Dedo
author_sort	Patricia García
title	Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast
title_short	Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast
title_full	Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast
title_fullStr	Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast
title_full_unstemmed	Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast
title_sort	eng2, a new player involved in feedback loop regulation of cdc42 activity in fission yeast
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/16260262ad454e01a138c3a4d10f29e0
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Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast

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