lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis

The main cause of steroid-induced necrosis of femoral head (SNFH) is excessive glucocorticoid (GC) intake. The aim of this article was to investigate the role of lncRNA NEAT1 as a molecular sponge to adsorb miR-23b-3p and regulate CYP1A2 in SNFH. Fluorescence in situ hybridization was used to locali...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhou Yongfang, Zhang Fei, Xu Fengyang, Wang Qiang, Wu Jianhua, Peng Wuxun, Dong Wentao
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	snfh lncrna neat1 hbmscs osteogenic differentiation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/162855b36bf84c3095cda91bcf862448
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:162855b36bf84c3095cda91bcf862448
record_format	dspace
spelling	oai:doaj.org-article:162855b36bf84c3095cda91bcf8624482021-12-05T14:10:41ZlncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis2391-541210.1515/biol-2021-0097https://doaj.org/article/162855b36bf84c3095cda91bcf8624482021-09-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0097https://doaj.org/toc/2391-5412The main cause of steroid-induced necrosis of femoral head (SNFH) is excessive glucocorticoid (GC) intake. The aim of this article was to investigate the role of lncRNA NEAT1 as a molecular sponge to adsorb miR-23b-3p and regulate CYP1A2 in SNFH. Fluorescence in situ hybridization was used to localize lncRNA NEAT1. Human bone marrow mesenchymal stem cells (hBMSCs) were collected from patients with SNFH. The expression of lncRNA NEAT1, miR-23b-3p and CYP1A2 in hBMSCs were intervened. Compared to the control group, the lncRNA NEAT1 and CYP1A2 expression in the SNFH group was increased, while miR-23b-3p expression was decreased. GCs could inhibit the osteogenic differentiation of hBMSCs and upregulate the expression of lncRNA NEAT1. Knockdown of lncRNA NEAT1 could promote the proliferation and osteogenic differentiation of hBMSCs in the SNFH group. Overexpression of miR-23b-3p could partially counteract the effect of lncRNA NEAT1 on hBMSCs. CYP1A2 was confirmed to be a target of miR-23b-3p. Overexpression of CYP1A2 could partially rescue the effect of miR-23b-3p overexpression on hBMSCs. In conclusion, lncRNA NEAT1 as a ceRNA can adsorb miR-23b-3p and promote the expression of CYP1A2, which then inhibits the osteogenic differentiation of hBMSCs and promotes the progress of SNFH.Zhou YongfangZhang FeiXu FengyangWang QiangWu JianhuaPeng WuxunDong WentaoDe Gruyterarticlesnfhlncrna neat1hbmscsosteogenic differentiationBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 969-980 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	snfh lncrna neat1 hbmscs osteogenic differentiation Biology (General) QH301-705.5
spellingShingle	snfh lncrna neat1 hbmscs osteogenic differentiation Biology (General) QH301-705.5 Zhou Yongfang Zhang Fei Xu Fengyang Wang Qiang Wu Jianhua Peng Wuxun Dong Wentao lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis
description	The main cause of steroid-induced necrosis of femoral head (SNFH) is excessive glucocorticoid (GC) intake. The aim of this article was to investigate the role of lncRNA NEAT1 as a molecular sponge to adsorb miR-23b-3p and regulate CYP1A2 in SNFH. Fluorescence in situ hybridization was used to localize lncRNA NEAT1. Human bone marrow mesenchymal stem cells (hBMSCs) were collected from patients with SNFH. The expression of lncRNA NEAT1, miR-23b-3p and CYP1A2 in hBMSCs were intervened. Compared to the control group, the lncRNA NEAT1 and CYP1A2 expression in the SNFH group was increased, while miR-23b-3p expression was decreased. GCs could inhibit the osteogenic differentiation of hBMSCs and upregulate the expression of lncRNA NEAT1. Knockdown of lncRNA NEAT1 could promote the proliferation and osteogenic differentiation of hBMSCs in the SNFH group. Overexpression of miR-23b-3p could partially counteract the effect of lncRNA NEAT1 on hBMSCs. CYP1A2 was confirmed to be a target of miR-23b-3p. Overexpression of CYP1A2 could partially rescue the effect of miR-23b-3p overexpression on hBMSCs. In conclusion, lncRNA NEAT1 as a ceRNA can adsorb miR-23b-3p and promote the expression of CYP1A2, which then inhibits the osteogenic differentiation of hBMSCs and promotes the progress of SNFH.
format	article
author	Zhou Yongfang Zhang Fei Xu Fengyang Wang Qiang Wu Jianhua Peng Wuxun Dong Wentao
author_facet	Zhou Yongfang Zhang Fei Xu Fengyang Wang Qiang Wu Jianhua Peng Wuxun Dong Wentao
author_sort	Zhou Yongfang
title	lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis
title_short	lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis
title_full	lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis
title_fullStr	lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis
title_full_unstemmed	lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis
title_sort	lncrna neat1 regulates cyp1a2 and influences steroid-induced necrosis
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/162855b36bf84c3095cda91bcf862448
work_keys_str_mv	AT zhouyongfang lncrnaneat1regulatescyp1a2andinfluencessteroidinducednecrosis AT zhangfei lncrnaneat1regulatescyp1a2andinfluencessteroidinducednecrosis AT xufengyang lncrnaneat1regulatescyp1a2andinfluencessteroidinducednecrosis AT wangqiang lncrnaneat1regulatescyp1a2andinfluencessteroidinducednecrosis AT wujianhua lncrnaneat1regulatescyp1a2andinfluencessteroidinducednecrosis AT pengwuxun lncrnaneat1regulatescyp1a2andinfluencessteroidinducednecrosis AT dongwentao lncrnaneat1regulatescyp1a2andinfluencessteroidinducednecrosis
_version_	1718371825335926784

lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis

Ejemplares similares