In vitro culture physiology
The culture procedures described up to the eighties, did not made any mention to the environmental control of in vitro plant development. However, growth rate, development and many of the physiologic-morphologic features of the in vitro grown plants are influenced by the culture vessel. The increa...
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Universidad Central Marta Abreu de Las Villas
2001
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oai:doaj.org-article:163196e01ad04c7a9e1f8871ad7c4e1d2021-11-23T15:54:56ZIn vitro culture physiology1609-18412074-8647https://doaj.org/article/163196e01ad04c7a9e1f8871ad7c4e1d2001-03-01T00:00:00Zhttps://revista.ibp.co.cu/index.php/BV/article/view/59https://doaj.org/toc/1609-1841https://doaj.org/toc/2074-8647The culture procedures described up to the eighties, did not made any mention to the environmental control of in vitro plant development. However, growth rate, development and many of the physiologic-morphologic features of the in vitro grown plants are influenced by the culture vessel. The increasing knowledge about the environmental control of culture vessels under sterile conditions, is helping to change micorpropagation procedures. The in vitro environment with lower rate ventilation, brings about low flow rates of matter and energy, with minimum variations of temperature, high relative humidity and large daily changes of the concentration of CO 2 inside the culture vessel. The type of culture vessel (size, shape, fabric and closing system) can influence the evolution of the atmosphere along the time of culture. Although submitted to different stresses factors plant can be grown in vitro, but plants can be faulty in their anatomy, morphology and physiology. As a consequence, these plants shown a phenotype unable to survive to ex vitro conditions. Different strategies can be used to control the atmosphere along the different phases of micropropagation, in heterotrophic, mixotrophic or autotrophic cultures. The election of the best strategy will be based on different factors as species, number of transplantes required, or quality-price relationship. Maria Jesús CañalRoberto Rodríguez Belén Fernández Ricardo Sánchez-TamesJuan Pedro MajadaUniversidad Central Marta Abreu de Las Villasarticlein vitro enviromentalcharacteristic physiologyenviromental controltissue culturemicropropagationAgricultureSPlant cultureSB1-1110ENESBiotecnología Vegetal, Vol 1, Iss 1, Pp 3-9 (2001) |
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DOAJ |
| collection |
DOAJ |
| language |
EN ES |
| topic |
in vitro enviromental characteristic physiology enviromental control tissue culture micropropagation Agriculture S Plant culture SB1-1110 |
| spellingShingle |
in vitro enviromental characteristic physiology enviromental control tissue culture micropropagation Agriculture S Plant culture SB1-1110 Maria Jesús Cañal Roberto Rodríguez Belén Fernández Ricardo Sánchez-Tames Juan Pedro Majada In vitro culture physiology |
| description |
The culture procedures described up to the eighties, did not made any mention to the environmental control of in vitro
plant development. However, growth rate, development and many of the physiologic-morphologic features of the in vitro
grown plants are influenced by the culture vessel. The increasing knowledge about the environmental control of culture
vessels under sterile conditions, is helping to change micorpropagation procedures. The in vitro environment with
lower rate ventilation, brings about low flow rates of matter and energy, with minimum variations of temperature, high
relative humidity and large daily changes of the concentration of CO 2 inside the culture vessel. The type of culture vessel
(size, shape, fabric and closing system) can influence the evolution of the atmosphere along the time of culture.
Although submitted to different stresses factors plant can be grown in vitro, but plants can be faulty in their anatomy,
morphology and physiology. As a consequence, these plants shown a phenotype unable to survive to ex vitro conditions.
Different strategies can be used to control the atmosphere along the different phases of micropropagation, in
heterotrophic, mixotrophic or autotrophic cultures. The election of the best strategy will be based on different factors as
species, number of transplantes required, or quality-price relationship.
|
| format |
article |
| author |
Maria Jesús Cañal Roberto Rodríguez Belén Fernández Ricardo Sánchez-Tames Juan Pedro Majada |
| author_facet |
Maria Jesús Cañal Roberto Rodríguez Belén Fernández Ricardo Sánchez-Tames Juan Pedro Majada |
| author_sort |
Maria Jesús Cañal |
| title |
In vitro culture physiology |
| title_short |
In vitro culture physiology |
| title_full |
In vitro culture physiology |
| title_fullStr |
In vitro culture physiology |
| title_full_unstemmed |
In vitro culture physiology |
| title_sort |
in vitro culture physiology |
| publisher |
Universidad Central Marta Abreu de Las Villas |
| publishDate |
2001 |
| url |
https://doaj.org/article/163196e01ad04c7a9e1f8871ad7c4e1d |
| work_keys_str_mv |
AT mariajesuscanal invitroculturephysiology AT robertorodriguez invitroculturephysiology AT belenfernandez invitroculturephysiology AT ricardosancheztames invitroculturephysiology AT juanpedromajada invitroculturephysiology |
| _version_ |
1718416196487872512 |