Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.

The directional movement and positioning of organelles and macromolecules is essential for regulating and maintaining cellular functions in eukaryotic cells. In plants, these processes are actin-based and driven by class XI myosins, which transport various cargos in a directed manner. As the analysi...

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Autores principales:	Lisa Stephan, Marc Jakoby, Arijit Das, Eva Koebke, Martin Hülskamp
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16375288069c4be083dad7b18e903712
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spelling	oai:doaj.org-article:16375288069c4be083dad7b18e9037122021-12-02T20:05:29ZUnravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.1932-620310.1371/journal.pone.0252327https://doaj.org/article/16375288069c4be083dad7b18e9037122021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252327https://doaj.org/toc/1932-6203The directional movement and positioning of organelles and macromolecules is essential for regulating and maintaining cellular functions in eukaryotic cells. In plants, these processes are actin-based and driven by class XI myosins, which transport various cargos in a directed manner. As the analysis of myosin function is challenging due to high levels of redundancy, dominant negative acting truncated myosins have frequently been used to study intracellular transport processes. A comparison of the dominant negative effect of the coiled-coil domains and the GTD domains revealed a much stronger inhibition of P-body movement by the GTD domains. In addition, we show that the GTD domain does not inhibit P-body movement when driven by a hybrid myosin in which the GTD domain was replaced by DCP2. These data suggest that the dominant negative effect of myosin tails involves a competition of the GTD domains for cargo binding sites.Lisa StephanMarc JakobyArijit DasEva KoebkeMartin HülskampPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0252327 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lisa Stephan Marc Jakoby Arijit Das Eva Koebke Martin Hülskamp Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.
description	The directional movement and positioning of organelles and macromolecules is essential for regulating and maintaining cellular functions in eukaryotic cells. In plants, these processes are actin-based and driven by class XI myosins, which transport various cargos in a directed manner. As the analysis of myosin function is challenging due to high levels of redundancy, dominant negative acting truncated myosins have frequently been used to study intracellular transport processes. A comparison of the dominant negative effect of the coiled-coil domains and the GTD domains revealed a much stronger inhibition of P-body movement by the GTD domains. In addition, we show that the GTD domain does not inhibit P-body movement when driven by a hybrid myosin in which the GTD domain was replaced by DCP2. These data suggest that the dominant negative effect of myosin tails involves a competition of the GTD domains for cargo binding sites.
format	article
author	Lisa Stephan Marc Jakoby Arijit Das Eva Koebke Martin Hülskamp
author_facet	Lisa Stephan Marc Jakoby Arijit Das Eva Koebke Martin Hülskamp
author_sort	Lisa Stephan
title	Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.
title_short	Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.
title_full	Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.
title_fullStr	Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.
title_full_unstemmed	Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.
title_sort	unravelling the molecular basis of the dominant negative effect of myosin xi tails on p-bodies.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/16375288069c4be083dad7b18e903712
work_keys_str_mv	AT lisastephan unravellingthemolecularbasisofthedominantnegativeeffectofmyosinxitailsonpbodies AT marcjakoby unravellingthemolecularbasisofthedominantnegativeeffectofmyosinxitailsonpbodies AT arijitdas unravellingthemolecularbasisofthedominantnegativeeffectofmyosinxitailsonpbodies AT evakoebke unravellingthemolecularbasisofthedominantnegativeeffectofmyosinxitailsonpbodies AT martinhulskamp unravellingthemolecularbasisofthedominantnegativeeffectofmyosinxitailsonpbodies
_version_	1718375467715657728

Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.

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