Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging

Abstract Using two-dimensional top-down view microscopy, researchers have recently described chondrocytes as being spatially arranged in distinct patterns such as strings, double strings, and small and large clusters. Because of the seeming association of these changes with tissue degeneration, they...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marina Danalache, Kevin Ralf Beutler, Bernd Rolauffs, Julius Michael Wolfgart, Florian Christof Bonnaire, Stefan Fischer, Imke Greving, Ulf Krister Hofmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1639485ef297488cbcc2c013107d0660
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1639485ef297488cbcc2c013107d0660
record_format	dspace
spelling	oai:doaj.org-article:1639485ef297488cbcc2c013107d06602021-12-02T14:29:15ZExploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging10.1038/s41598-021-89582-w2045-2322https://doaj.org/article/1639485ef297488cbcc2c013107d06602021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89582-whttps://doaj.org/toc/2045-2322Abstract Using two-dimensional top-down view microscopy, researchers have recently described chondrocytes as being spatially arranged in distinct patterns such as strings, double strings, and small and large clusters. Because of the seeming association of these changes with tissue degeneration, they have been proposed as an image-based biomarker for early osteoarthritis (OA) staging. The aim of our study was to investigate the spatial arrangement of chondrocytes in human articular cartilage in a 3D fashion and to evaluate the 3D changes of these patterns in the context of local tissue destruction. Decalcified femoral condyle resections from the load-bearing area were analysed in 3D for their spatial chondrocyte organisation by means of fluorescence microscopy and synchrotron-radiation micro-computed tomography (SR-µCT). In intact cartilage chondrocyte strings can be found in the superficial, transitional and deep zones. The proposed pattern changes accompanying tissue destruction could be located not just along the surface but also through all layers of cartilage. Each spatial pattern was characterised by a different cellular density (the only exception being between single and double strings with p = 0.062), with cellular density significantly increasing alongside the increase in local tissue degeneration as defined by the chondrocyte patterns. We can thus corroborate that the proposed cellular spatial changes are a three-dimensional function of local tissue degeneration, underlining their relevance as an image-based biomarker for the early diagnosis and description of OA. Clinical trial registration number: Project number of the ethics committee of the University of Tübingen:171/2014BO2.Marina DanalacheKevin Ralf BeutlerBernd RolauffsJulius Michael WolfgartFlorian Christof BonnaireStefan FischerImke GrevingUlf Krister HofmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marina Danalache Kevin Ralf Beutler Bernd Rolauffs Julius Michael Wolfgart Florian Christof Bonnaire Stefan Fischer Imke Greving Ulf Krister Hofmann Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging
description	Abstract Using two-dimensional top-down view microscopy, researchers have recently described chondrocytes as being spatially arranged in distinct patterns such as strings, double strings, and small and large clusters. Because of the seeming association of these changes with tissue degeneration, they have been proposed as an image-based biomarker for early osteoarthritis (OA) staging. The aim of our study was to investigate the spatial arrangement of chondrocytes in human articular cartilage in a 3D fashion and to evaluate the 3D changes of these patterns in the context of local tissue destruction. Decalcified femoral condyle resections from the load-bearing area were analysed in 3D for their spatial chondrocyte organisation by means of fluorescence microscopy and synchrotron-radiation micro-computed tomography (SR-µCT). In intact cartilage chondrocyte strings can be found in the superficial, transitional and deep zones. The proposed pattern changes accompanying tissue destruction could be located not just along the surface but also through all layers of cartilage. Each spatial pattern was characterised by a different cellular density (the only exception being between single and double strings with p = 0.062), with cellular density significantly increasing alongside the increase in local tissue degeneration as defined by the chondrocyte patterns. We can thus corroborate that the proposed cellular spatial changes are a three-dimensional function of local tissue degeneration, underlining their relevance as an image-based biomarker for the early diagnosis and description of OA. Clinical trial registration number: Project number of the ethics committee of the University of Tübingen:171/2014BO2.
format	article
author	Marina Danalache Kevin Ralf Beutler Bernd Rolauffs Julius Michael Wolfgart Florian Christof Bonnaire Stefan Fischer Imke Greving Ulf Krister Hofmann
author_facet	Marina Danalache Kevin Ralf Beutler Bernd Rolauffs Julius Michael Wolfgart Florian Christof Bonnaire Stefan Fischer Imke Greving Ulf Krister Hofmann
author_sort	Marina Danalache
title	Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging
title_short	Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging
title_full	Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging
title_fullStr	Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging
title_full_unstemmed	Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging
title_sort	exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3d imaging
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1639485ef297488cbcc2c013107d0660
work_keys_str_mv	AT marinadanalache explorationofchangesinspatialchondrocyteorganisationinhumanosteoarthriticcartilagebymeansof3dimaging AT kevinralfbeutler explorationofchangesinspatialchondrocyteorganisationinhumanosteoarthriticcartilagebymeansof3dimaging AT berndrolauffs explorationofchangesinspatialchondrocyteorganisationinhumanosteoarthriticcartilagebymeansof3dimaging AT juliusmichaelwolfgart explorationofchangesinspatialchondrocyteorganisationinhumanosteoarthriticcartilagebymeansof3dimaging AT florianchristofbonnaire explorationofchangesinspatialchondrocyteorganisationinhumanosteoarthriticcartilagebymeansof3dimaging AT stefanfischer explorationofchangesinspatialchondrocyteorganisationinhumanosteoarthriticcartilagebymeansof3dimaging AT imkegreving explorationofchangesinspatialchondrocyteorganisationinhumanosteoarthriticcartilagebymeansof3dimaging AT ulfkristerhofmann explorationofchangesinspatialchondrocyteorganisationinhumanosteoarthriticcartilagebymeansof3dimaging
_version_	1718391175653621760

Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging

Ejemplares similares