Potential Prognostic Biomarkers of NIMA (Never in Mitosis, Gene A)-Related Kinase (NEK) Family Members in Breast Cancer

Breast cancer remains the most common malignant cancer in women, with a staggering incidence of two million cases annually worldwide; therefore, it is crucial to explore novel biomarkers to assess the diagnosis and prognosis of breast cancer patients. NIMA-related kinase (NEK) protein kinase contain...

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Autores principales:	Gangga Anuraga, Wei-Jan Wang, Nam Nhut Phan, Nu Thuy An Ton, Hoang Dang Khoa Ta, Fidelia Berenice Prayugo, Do Thi Minh Xuan, Su-Chi Ku, Yung-Fu Wu, Vivin Andriani, Muhammad Athoillah, Kuen-Haur Lee, Chih-Yang Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	breast cancer bioinformatics biomarker prognosis <i>NEK</i> family genes immune microenvironment Medicine R
Acceso en línea:	https://doaj.org/article/1640d087d2354af3ac2a849d445bda8e
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spelling	oai:doaj.org-article:1640d087d2354af3ac2a849d445bda8e2021-11-25T18:07:04ZPotential Prognostic Biomarkers of NIMA (Never in Mitosis, Gene A)-Related Kinase (NEK) Family Members in Breast Cancer10.3390/jpm111110892075-4426https://doaj.org/article/1640d087d2354af3ac2a849d445bda8e2021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4426/11/11/1089https://doaj.org/toc/2075-4426Breast cancer remains the most common malignant cancer in women, with a staggering incidence of two million cases annually worldwide; therefore, it is crucial to explore novel biomarkers to assess the diagnosis and prognosis of breast cancer patients. NIMA-related kinase (NEK) protein kinase contains 11 family members named NEK1-NEK11, which were discovered from <i>Aspergillus Nidulans</i>; however, the role of NEK family genes for tumor development remains unclear and requires additional study. In the present study, we investigate the prognosis relationships of NEK family genes for breast cancer development, as well as the gene expression signature via the bioinformatics approach. The results of several integrative analyses revealed that most of the NEK family genes are overexpressed in breast cancer. Among these family genes, <i>NEK2/6/8</i> overexpression had poor prognostic significance in distant metastasis-free survival (DMFS) in breast cancer patients. Meanwhile, <i>NEK2/6</i> had the highest level of DNA methylation, and the functional enrichment analysis from MetaCore and Gene Set Enrichment Analysis (GSEA) suggested that <i>NEK2</i> was associated with the cell cycle, G2M checkpoint, DNA repair, E2F, MYC, MTORC1, and interferon-related signaling. Moreover, Tumor Immune Estimation Resource (TIMER) results showed that the transcriptional levels of NEK2 were positively correlated with immune infiltration of B cells and CD4<sup>+</sup> T Cell. Collectively, the current study indicated that <i>NEK</i> family genes, especially <i>NEK2</i> which is involved in immune infiltration, and may serve as prognosis biomarkers for breast cancer progression.Gangga AnuragaWei-Jan WangNam Nhut PhanNu Thuy An TonHoang Dang Khoa TaFidelia Berenice PrayugoDo Thi Minh XuanSu-Chi KuYung-Fu WuVivin AndrianiMuhammad AthoillahKuen-Haur LeeChih-Yang WangMDPI AGarticlebreast cancerbioinformaticsbiomarkerprognosis<i>NEK</i> family genesimmune microenvironmentMedicineRENJournal of Personalized Medicine, Vol 11, Iss 1089, p 1089 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer bioinformatics biomarker prognosis <i>NEK</i> family genes immune microenvironment Medicine R
spellingShingle	breast cancer bioinformatics biomarker prognosis <i>NEK</i> family genes immune microenvironment Medicine R Gangga Anuraga Wei-Jan Wang Nam Nhut Phan Nu Thuy An Ton Hoang Dang Khoa Ta Fidelia Berenice Prayugo Do Thi Minh Xuan Su-Chi Ku Yung-Fu Wu Vivin Andriani Muhammad Athoillah Kuen-Haur Lee Chih-Yang Wang Potential Prognostic Biomarkers of NIMA (Never in Mitosis, Gene A)-Related Kinase (NEK) Family Members in Breast Cancer
description	Breast cancer remains the most common malignant cancer in women, with a staggering incidence of two million cases annually worldwide; therefore, it is crucial to explore novel biomarkers to assess the diagnosis and prognosis of breast cancer patients. NIMA-related kinase (NEK) protein kinase contains 11 family members named NEK1-NEK11, which were discovered from <i>Aspergillus Nidulans</i>; however, the role of NEK family genes for tumor development remains unclear and requires additional study. In the present study, we investigate the prognosis relationships of NEK family genes for breast cancer development, as well as the gene expression signature via the bioinformatics approach. The results of several integrative analyses revealed that most of the NEK family genes are overexpressed in breast cancer. Among these family genes, <i>NEK2/6/8</i> overexpression had poor prognostic significance in distant metastasis-free survival (DMFS) in breast cancer patients. Meanwhile, <i>NEK2/6</i> had the highest level of DNA methylation, and the functional enrichment analysis from MetaCore and Gene Set Enrichment Analysis (GSEA) suggested that <i>NEK2</i> was associated with the cell cycle, G2M checkpoint, DNA repair, E2F, MYC, MTORC1, and interferon-related signaling. Moreover, Tumor Immune Estimation Resource (TIMER) results showed that the transcriptional levels of NEK2 were positively correlated with immune infiltration of B cells and CD4<sup>+</sup> T Cell. Collectively, the current study indicated that <i>NEK</i> family genes, especially <i>NEK2</i> which is involved in immune infiltration, and may serve as prognosis biomarkers for breast cancer progression.
format	article
author	Gangga Anuraga Wei-Jan Wang Nam Nhut Phan Nu Thuy An Ton Hoang Dang Khoa Ta Fidelia Berenice Prayugo Do Thi Minh Xuan Su-Chi Ku Yung-Fu Wu Vivin Andriani Muhammad Athoillah Kuen-Haur Lee Chih-Yang Wang
author_facet	Gangga Anuraga Wei-Jan Wang Nam Nhut Phan Nu Thuy An Ton Hoang Dang Khoa Ta Fidelia Berenice Prayugo Do Thi Minh Xuan Su-Chi Ku Yung-Fu Wu Vivin Andriani Muhammad Athoillah Kuen-Haur Lee Chih-Yang Wang
author_sort	Gangga Anuraga
title	Potential Prognostic Biomarkers of NIMA (Never in Mitosis, Gene A)-Related Kinase (NEK) Family Members in Breast Cancer
title_short	Potential Prognostic Biomarkers of NIMA (Never in Mitosis, Gene A)-Related Kinase (NEK) Family Members in Breast Cancer
title_full	Potential Prognostic Biomarkers of NIMA (Never in Mitosis, Gene A)-Related Kinase (NEK) Family Members in Breast Cancer
title_fullStr	Potential Prognostic Biomarkers of NIMA (Never in Mitosis, Gene A)-Related Kinase (NEK) Family Members in Breast Cancer
title_full_unstemmed	Potential Prognostic Biomarkers of NIMA (Never in Mitosis, Gene A)-Related Kinase (NEK) Family Members in Breast Cancer
title_sort	potential prognostic biomarkers of nima (never in mitosis, gene a)-related kinase (nek) family members in breast cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1640d087d2354af3ac2a849d445bda8e
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Potential Prognostic Biomarkers of NIMA (Never in Mitosis, Gene A)-Related Kinase (NEK) Family Members in Breast Cancer

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