Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation

The transient receptor potential vanilloid 2 (TRPV2) ion channel is activated by a chemical ligand (2-aminoethoxydiphenyl borate; 2-APB), noxious heat and mechanical stimulation. In a heterologous mammalian cell expression system, the oxidant chloramine T (ChT) sensitizes TRPV2 activation in respons...

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Autores principales:	Mai Oda, Yuichiro Fujiwara, Yasuki Ishizaki, Koji Shibasaki
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	TRP channel TRPV2 Oxidation Heat Mechanical stimulus Two-electrode voltage clamp method Biology (General) QH301-705.5 Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/1648b014572d4b7991b1212b02522703
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spelling	oai:doaj.org-article:1648b014572d4b7991b1212b025227032021-11-20T05:07:48ZOxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation2405-580810.1016/j.bbrep.2021.101173https://doaj.org/article/1648b014572d4b7991b1212b025227032021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405580821002673https://doaj.org/toc/2405-5808The transient receptor potential vanilloid 2 (TRPV2) ion channel is activated by a chemical ligand (2-aminoethoxydiphenyl borate; 2-APB), noxious heat and mechanical stimulation. In a heterologous mammalian cell expression system, the oxidant chloramine T (ChT) sensitizes TRPV2 activation in response to 2-APB and heat by oxidation of methionine residues at positions 528 and 607 in rat TRPV2. Here, we used a Xenopus oocyte expression system to determine whether ChT-mediated oxidation can also sensitize TRPV2 to mechanical stimulation. In this system, we confirmed that ChT sensitized TRPV2 activation in response to 2-APB and heat, but we detected no sensitization to mechanical stimulation. This result suggests that the activation mechanism of TRPV2 by a chemical ligand and heat differs from that for mechanical stimulation. Further, we demonstrated that two-electrode voltage clamp recording in the Xenopus oocyte expression system is an excellent format for high throughput analysis of oxidization of redox-sensitive TRP channels.Mai OdaYuichiro FujiwaraYasuki IshizakiKoji ShibasakiElsevierarticleTRP channelTRPV2OxidationHeatMechanical stimulusTwo-electrode voltage clamp methodBiology (General)QH301-705.5BiochemistryQD415-436ENBiochemistry and Biophysics Reports, Vol 28, Iss , Pp 101173- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TRP channel TRPV2 Oxidation Heat Mechanical stimulus Two-electrode voltage clamp method Biology (General) QH301-705.5 Biochemistry QD415-436
spellingShingle	TRP channel TRPV2 Oxidation Heat Mechanical stimulus Two-electrode voltage clamp method Biology (General) QH301-705.5 Biochemistry QD415-436 Mai Oda Yuichiro Fujiwara Yasuki Ishizaki Koji Shibasaki Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation
description	The transient receptor potential vanilloid 2 (TRPV2) ion channel is activated by a chemical ligand (2-aminoethoxydiphenyl borate; 2-APB), noxious heat and mechanical stimulation. In a heterologous mammalian cell expression system, the oxidant chloramine T (ChT) sensitizes TRPV2 activation in response to 2-APB and heat by oxidation of methionine residues at positions 528 and 607 in rat TRPV2. Here, we used a Xenopus oocyte expression system to determine whether ChT-mediated oxidation can also sensitize TRPV2 to mechanical stimulation. In this system, we confirmed that ChT sensitized TRPV2 activation in response to 2-APB and heat, but we detected no sensitization to mechanical stimulation. This result suggests that the activation mechanism of TRPV2 by a chemical ligand and heat differs from that for mechanical stimulation. Further, we demonstrated that two-electrode voltage clamp recording in the Xenopus oocyte expression system is an excellent format for high throughput analysis of oxidization of redox-sensitive TRP channels.
format	article
author	Mai Oda Yuichiro Fujiwara Yasuki Ishizaki Koji Shibasaki
author_facet	Mai Oda Yuichiro Fujiwara Yasuki Ishizaki Koji Shibasaki
author_sort	Mai Oda
title	Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation
title_short	Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation
title_full	Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation
title_fullStr	Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation
title_full_unstemmed	Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation
title_sort	oxidation sensitizes trpv2 to chemical and heat stimuli, but not mechanical stimulation
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/1648b014572d4b7991b1212b02522703
work_keys_str_mv	AT maioda oxidationsensitizestrpv2tochemicalandheatstimulibutnotmechanicalstimulation AT yuichirofujiwara oxidationsensitizestrpv2tochemicalandheatstimulibutnotmechanicalstimulation AT yasukiishizaki oxidationsensitizestrpv2tochemicalandheatstimulibutnotmechanicalstimulation AT kojishibasaki oxidationsensitizestrpv2tochemicalandheatstimulibutnotmechanicalstimulation
_version_	1718419526753714176

Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation

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