Analysis of global sumoylation changes occurring during keratinocyte differentiation.

Sumoylation is a highly dynamic process that plays a role in a multitude of processes ranging from cell cycle progression to mRNA processing and cancer. A previous study from our lab demonstrated that SUMO plays an important role in keratinocyte differentiation. Here we present a new method of track...

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Autores principales:	Phillip R Heaton, Andres Santos, Germán Rosas-Acosta, Van G Wilson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1649d13eb40449cfac2a0750414b9773
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spelling	oai:doaj.org-article:1649d13eb40449cfac2a0750414b97732021-11-18T07:29:39ZAnalysis of global sumoylation changes occurring during keratinocyte differentiation.1932-620310.1371/journal.pone.0030165https://doaj.org/article/1649d13eb40449cfac2a0750414b97732012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22291911/?tool=EBIhttps://doaj.org/toc/1932-6203Sumoylation is a highly dynamic process that plays a role in a multitude of processes ranging from cell cycle progression to mRNA processing and cancer. A previous study from our lab demonstrated that SUMO plays an important role in keratinocyte differentiation. Here we present a new method of tracking the sumoylation state of proteins by creating a stably transfected HaCaT keratinocyte cell line expressing an inducible SNAP-SUMO3 protein. The SNAP-tag allows covalent fluorescent labeling that is denaturation resistant. When combined with two-dimensional gel electrophoresis, the SNAP-tag technology provides direct visualization of sumoylated targets and can be used to follow temporal changes in the global cohort of sumoylated proteins during dynamic processes such as differentiation. HaCaT keratinocyte cells expressing SNAP-SUMO3 displayed normal morphological and biochemical features that are consistent with typical keratinocyte differentiation. SNAP-SUMO3 also localized normally in these cells with a predominantly nuclear signal and some minor cytoplasmic staining, consistent with previous reports for untagged SUMO2/3. During keratinocyte differentiation the total number of proteins modified by SNAP-SUMO3 was highest in basal cells, decreased abruptly after induction of differentiation, and slowly rebounded beginning between 48 and 72 hours as differentiation progressed. However, within this overall trend the pattern of change for individual sumoylated proteins was highly variable with both increases and decreases in amount over time. From these results we conclude that sumoylation of proteins during keratinocyte differentiation is a complex process which likely reflects and contributes to the biochemical changes that drive differentiation.Phillip R HeatonAndres SantosGermán Rosas-AcostaVan G WilsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30165 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Phillip R Heaton Andres Santos Germán Rosas-Acosta Van G Wilson Analysis of global sumoylation changes occurring during keratinocyte differentiation.
description	Sumoylation is a highly dynamic process that plays a role in a multitude of processes ranging from cell cycle progression to mRNA processing and cancer. A previous study from our lab demonstrated that SUMO plays an important role in keratinocyte differentiation. Here we present a new method of tracking the sumoylation state of proteins by creating a stably transfected HaCaT keratinocyte cell line expressing an inducible SNAP-SUMO3 protein. The SNAP-tag allows covalent fluorescent labeling that is denaturation resistant. When combined with two-dimensional gel electrophoresis, the SNAP-tag technology provides direct visualization of sumoylated targets and can be used to follow temporal changes in the global cohort of sumoylated proteins during dynamic processes such as differentiation. HaCaT keratinocyte cells expressing SNAP-SUMO3 displayed normal morphological and biochemical features that are consistent with typical keratinocyte differentiation. SNAP-SUMO3 also localized normally in these cells with a predominantly nuclear signal and some minor cytoplasmic staining, consistent with previous reports for untagged SUMO2/3. During keratinocyte differentiation the total number of proteins modified by SNAP-SUMO3 was highest in basal cells, decreased abruptly after induction of differentiation, and slowly rebounded beginning between 48 and 72 hours as differentiation progressed. However, within this overall trend the pattern of change for individual sumoylated proteins was highly variable with both increases and decreases in amount over time. From these results we conclude that sumoylation of proteins during keratinocyte differentiation is a complex process which likely reflects and contributes to the biochemical changes that drive differentiation.
format	article
author	Phillip R Heaton Andres Santos Germán Rosas-Acosta Van G Wilson
author_facet	Phillip R Heaton Andres Santos Germán Rosas-Acosta Van G Wilson
author_sort	Phillip R Heaton
title	Analysis of global sumoylation changes occurring during keratinocyte differentiation.
title_short	Analysis of global sumoylation changes occurring during keratinocyte differentiation.
title_full	Analysis of global sumoylation changes occurring during keratinocyte differentiation.
title_fullStr	Analysis of global sumoylation changes occurring during keratinocyte differentiation.
title_full_unstemmed	Analysis of global sumoylation changes occurring during keratinocyte differentiation.
title_sort	analysis of global sumoylation changes occurring during keratinocyte differentiation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1649d13eb40449cfac2a0750414b9773
work_keys_str_mv	AT philliprheaton analysisofglobalsumoylationchangesoccurringduringkeratinocytedifferentiation AT andressantos analysisofglobalsumoylationchangesoccurringduringkeratinocytedifferentiation AT germanrosasacosta analysisofglobalsumoylationchangesoccurringduringkeratinocytedifferentiation AT vangwilson analysisofglobalsumoylationchangesoccurringduringkeratinocytedifferentiation
_version_	1718423332375756800

Analysis of global sumoylation changes occurring during keratinocyte differentiation.

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