GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.

Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefi...

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Autores principales:	Alejandro Sarrion-Perdigones, Erica Elvira Falconi, Sara I Zandalinas, Paloma Juárez, Asun Fernández-del-Carmen, Antonio Granell, Diego Orzaez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/164a8a4a302342f6bba57010e59ea6a4
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spelling	oai:doaj.org-article:164a8a4a302342f6bba57010e59ea6a42021-11-18T06:50:38ZGoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.1932-620310.1371/journal.pone.0021622https://doaj.org/article/164a8a4a302342f6bba57010e59ea6a42011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21750718/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefinite growth of reusable gene modules made of standardized DNA pieces. The GB system consists of a set of four destination plasmids (pDGBs) designed to incorporate multipartite assemblies made of standard DNA parts and to combine them binarily to build increasingly complex multigene constructs. The relative position of type IIS restriction sites inside pDGB vectors introduces a double loop ("braid") topology in the cloning strategy that allows the indefinite growth of composite parts through the succession of iterative assembling steps, while the overall simplicity of the system is maintained. We propose the use of GoldenBraid as an assembly standard for Plant Synthetic Biology. For this purpose we have GB-adapted a set of binary plasmids for A. tumefaciens-mediated plant transformation. Fast GB-engineering of several multigene T-DNAs, including two alternative modules made of five reusable devices each, and comprising a total of 19 basic parts are also described.Alejandro Sarrion-PerdigonesErica Elvira FalconiSara I ZandalinasPaloma JuárezAsun Fernández-del-CarmenAntonio GranellDiego OrzaezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e21622 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alejandro Sarrion-Perdigones Erica Elvira Falconi Sara I Zandalinas Paloma Juárez Asun Fernández-del-Carmen Antonio Granell Diego Orzaez GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.
description	Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefinite growth of reusable gene modules made of standardized DNA pieces. The GB system consists of a set of four destination plasmids (pDGBs) designed to incorporate multipartite assemblies made of standard DNA parts and to combine them binarily to build increasingly complex multigene constructs. The relative position of type IIS restriction sites inside pDGB vectors introduces a double loop ("braid") topology in the cloning strategy that allows the indefinite growth of composite parts through the succession of iterative assembling steps, while the overall simplicity of the system is maintained. We propose the use of GoldenBraid as an assembly standard for Plant Synthetic Biology. For this purpose we have GB-adapted a set of binary plasmids for A. tumefaciens-mediated plant transformation. Fast GB-engineering of several multigene T-DNAs, including two alternative modules made of five reusable devices each, and comprising a total of 19 basic parts are also described.
format	article
author	Alejandro Sarrion-Perdigones Erica Elvira Falconi Sara I Zandalinas Paloma Juárez Asun Fernández-del-Carmen Antonio Granell Diego Orzaez
author_facet	Alejandro Sarrion-Perdigones Erica Elvira Falconi Sara I Zandalinas Paloma Juárez Asun Fernández-del-Carmen Antonio Granell Diego Orzaez
author_sort	Alejandro Sarrion-Perdigones
title	GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.
title_short	GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.
title_full	GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.
title_fullStr	GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.
title_full_unstemmed	GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.
title_sort	goldenbraid: an iterative cloning system for standardized assembly of reusable genetic modules.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/164a8a4a302342f6bba57010e59ea6a4
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GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.

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