Further evidence supporting a potential role for ADH1B in obesity
Abstract Insulin is an essential hormone that regulates glucose homeostasis and metabolism. Insulin resistance (IR) arises when tissues fail to respond to insulin, and it leads to serious health problems including Type 2 Diabetes (T2D). Obesity is a major contributor to the development of IR and T2D...
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Nature Portfolio
2021
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oai:doaj.org-article:1659f6d5d12d42ebbff7be8f7ccaf33e2021-12-02T15:23:29ZFurther evidence supporting a potential role for ADH1B in obesity10.1038/s41598-020-80563-z2045-2322https://doaj.org/article/1659f6d5d12d42ebbff7be8f7ccaf33e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80563-zhttps://doaj.org/toc/2045-2322Abstract Insulin is an essential hormone that regulates glucose homeostasis and metabolism. Insulin resistance (IR) arises when tissues fail to respond to insulin, and it leads to serious health problems including Type 2 Diabetes (T2D). Obesity is a major contributor to the development of IR and T2D. We previously showed that gene expression of alcohol dehydrogenase 1B (ADH1B) was inversely correlated with obesity and IR in subcutaneous adipose tissue of Mexican Americans. In the current study, a meta-analysis of the relationship between ADH1B expression and BMI in Mexican Americans, African Americans, Europeans, and Pima Indians verified that BMI was increased with decreased ADH1B expression. Using established human subcutaneous pre-adipocyte cell lines derived from lean (BMI < 30 kg m−2) or obese (BMI ≥ 30 kg m−2) donors, we found that ADH1B protein expression increased substantially during differentiation, and overexpression of ADH1B inhibited fatty acid binding protein expression. Mature adipocytes from lean donors expressed ADH1B at higher levels than obese donors. Insulin further induced ADH1B protein expression as well as enzyme activity. Knockdown of ADH1B expression decreased insulin-stimulated glucose uptake. Our findings suggest that ADH1B is involved in the proper development and metabolic activity of adipose tissues and this function is suppressed by obesity.Liza D. MoralesDouglas T. CromackDevjit TripathyMarcel FourcaudotSatish KumarJoanne E. CurranMelanie CarlessHarald H. H. GöringShirley L. HuJuan Carlos Lopez-AlvarengaKristina M. GarskePäivi PajukantaKerrin S. SmallCraig A. GlastonburySwapan K. DasCarl LangefeldRobert L. HansonWen-Chi HsuehLuke NortonRector AryaSrinivas MummidiJohn BlangeroRalph A. DeFronzoRavindranath DuggiralaChristopher P. JenkinsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
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Medicine R Science Q Liza D. Morales Douglas T. Cromack Devjit Tripathy Marcel Fourcaudot Satish Kumar Joanne E. Curran Melanie Carless Harald H. H. Göring Shirley L. Hu Juan Carlos Lopez-Alvarenga Kristina M. Garske Päivi Pajukanta Kerrin S. Small Craig A. Glastonbury Swapan K. Das Carl Langefeld Robert L. Hanson Wen-Chi Hsueh Luke Norton Rector Arya Srinivas Mummidi John Blangero Ralph A. DeFronzo Ravindranath Duggirala Christopher P. Jenkinson Further evidence supporting a potential role for ADH1B in obesity |
| description |
Abstract Insulin is an essential hormone that regulates glucose homeostasis and metabolism. Insulin resistance (IR) arises when tissues fail to respond to insulin, and it leads to serious health problems including Type 2 Diabetes (T2D). Obesity is a major contributor to the development of IR and T2D. We previously showed that gene expression of alcohol dehydrogenase 1B (ADH1B) was inversely correlated with obesity and IR in subcutaneous adipose tissue of Mexican Americans. In the current study, a meta-analysis of the relationship between ADH1B expression and BMI in Mexican Americans, African Americans, Europeans, and Pima Indians verified that BMI was increased with decreased ADH1B expression. Using established human subcutaneous pre-adipocyte cell lines derived from lean (BMI < 30 kg m−2) or obese (BMI ≥ 30 kg m−2) donors, we found that ADH1B protein expression increased substantially during differentiation, and overexpression of ADH1B inhibited fatty acid binding protein expression. Mature adipocytes from lean donors expressed ADH1B at higher levels than obese donors. Insulin further induced ADH1B protein expression as well as enzyme activity. Knockdown of ADH1B expression decreased insulin-stimulated glucose uptake. Our findings suggest that ADH1B is involved in the proper development and metabolic activity of adipose tissues and this function is suppressed by obesity. |
| format |
article |
| author |
Liza D. Morales Douglas T. Cromack Devjit Tripathy Marcel Fourcaudot Satish Kumar Joanne E. Curran Melanie Carless Harald H. H. Göring Shirley L. Hu Juan Carlos Lopez-Alvarenga Kristina M. Garske Päivi Pajukanta Kerrin S. Small Craig A. Glastonbury Swapan K. Das Carl Langefeld Robert L. Hanson Wen-Chi Hsueh Luke Norton Rector Arya Srinivas Mummidi John Blangero Ralph A. DeFronzo Ravindranath Duggirala Christopher P. Jenkinson |
| author_facet |
Liza D. Morales Douglas T. Cromack Devjit Tripathy Marcel Fourcaudot Satish Kumar Joanne E. Curran Melanie Carless Harald H. H. Göring Shirley L. Hu Juan Carlos Lopez-Alvarenga Kristina M. Garske Päivi Pajukanta Kerrin S. Small Craig A. Glastonbury Swapan K. Das Carl Langefeld Robert L. Hanson Wen-Chi Hsueh Luke Norton Rector Arya Srinivas Mummidi John Blangero Ralph A. DeFronzo Ravindranath Duggirala Christopher P. Jenkinson |
| author_sort |
Liza D. Morales |
| title |
Further evidence supporting a potential role for ADH1B in obesity |
| title_short |
Further evidence supporting a potential role for ADH1B in obesity |
| title_full |
Further evidence supporting a potential role for ADH1B in obesity |
| title_fullStr |
Further evidence supporting a potential role for ADH1B in obesity |
| title_full_unstemmed |
Further evidence supporting a potential role for ADH1B in obesity |
| title_sort |
further evidence supporting a potential role for adh1b in obesity |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/1659f6d5d12d42ebbff7be8f7ccaf33e |
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