AIF downregulation and its interaction with STK3 in renal cell carcinoma.

Apoptosis-inducing factor (AIF) plays a crucial role in caspase-independent programmed cell death by triggering chromatin condensation and DNA fragmentation. Therefore, it might be involved in cell homeostasis and tumor development. In this study, we report significant AIF downregulation in the majo...

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Autores principales:	Shengqiang Xu, Hongjin Wu, Huan Nie, Lei Yue, Huadong Jiang, Sheng Xiao, Yu Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/165cebf6a2614a30b53fb8da51d20722
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spelling	oai:doaj.org-article:165cebf6a2614a30b53fb8da51d207222021-11-25T06:09:42ZAIF downregulation and its interaction with STK3 in renal cell carcinoma.1932-620310.1371/journal.pone.0100824https://doaj.org/article/165cebf6a2614a30b53fb8da51d207222014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24992339/?tool=EBIhttps://doaj.org/toc/1932-6203Apoptosis-inducing factor (AIF) plays a crucial role in caspase-independent programmed cell death by triggering chromatin condensation and DNA fragmentation. Therefore, it might be involved in cell homeostasis and tumor development. In this study, we report significant AIF downregulation in the majority of renal cell carcinomas (RCC). In a group of RCC specimens, 84% (43 out of 51) had AIF downregulation by immunohistochemistry stain. Additional 10 kidney tumors, including an oxyphilic adenoma, also had significant AIF downregulation by Northern blot analysis. The mechanisms of the AIF downregulation included both AIF deletion and its promoter methylation. Forced expression of AIF in RCC cell lines induced massive apoptosis. Further analysis revealed that AIF interacted with STK3, a known regulator of apoptosis, and enhanced its phosphorylation at Thr180. These results suggest that AIF downregulation is a common event in kidney tumor development. AIF loss may lead to decreased STK3 activity, defective apoptosis and malignant transformation.Shengqiang XuHongjin WuHuan NieLei YueHuadong JiangSheng XiaoYu LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e100824 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shengqiang Xu Hongjin Wu Huan Nie Lei Yue Huadong Jiang Sheng Xiao Yu Li AIF downregulation and its interaction with STK3 in renal cell carcinoma.
description	Apoptosis-inducing factor (AIF) plays a crucial role in caspase-independent programmed cell death by triggering chromatin condensation and DNA fragmentation. Therefore, it might be involved in cell homeostasis and tumor development. In this study, we report significant AIF downregulation in the majority of renal cell carcinomas (RCC). In a group of RCC specimens, 84% (43 out of 51) had AIF downregulation by immunohistochemistry stain. Additional 10 kidney tumors, including an oxyphilic adenoma, also had significant AIF downregulation by Northern blot analysis. The mechanisms of the AIF downregulation included both AIF deletion and its promoter methylation. Forced expression of AIF in RCC cell lines induced massive apoptosis. Further analysis revealed that AIF interacted with STK3, a known regulator of apoptosis, and enhanced its phosphorylation at Thr180. These results suggest that AIF downregulation is a common event in kidney tumor development. AIF loss may lead to decreased STK3 activity, defective apoptosis and malignant transformation.
format	article
author	Shengqiang Xu Hongjin Wu Huan Nie Lei Yue Huadong Jiang Sheng Xiao Yu Li
author_facet	Shengqiang Xu Hongjin Wu Huan Nie Lei Yue Huadong Jiang Sheng Xiao Yu Li
author_sort	Shengqiang Xu
title	AIF downregulation and its interaction with STK3 in renal cell carcinoma.
title_short	AIF downregulation and its interaction with STK3 in renal cell carcinoma.
title_full	AIF downregulation and its interaction with STK3 in renal cell carcinoma.
title_fullStr	AIF downregulation and its interaction with STK3 in renal cell carcinoma.
title_full_unstemmed	AIF downregulation and its interaction with STK3 in renal cell carcinoma.
title_sort	aif downregulation and its interaction with stk3 in renal cell carcinoma.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/165cebf6a2614a30b53fb8da51d20722
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_version_	1718414089154199552

AIF downregulation and its interaction with STK3 in renal cell carcinoma.

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