Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera

Abstract Chagas disease is considered the most important parasitic disease in Latin America. The protozoan agent, Trypanosoma cruzi, comprises six genetic lineages, TcI-TcVI. Genotyping to link lineage(s) to severity of cardiomyopathy and gastrointestinal pathology is impeded by the sequestration an...

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Autores principales:	Niamh Murphy, Barrie Rooney, Tapan Bhattacharyya, Omar Triana-Chavez, Anja Krueger, Stuart M. Haslam, Victoria O’Rourke, Magdalena Pańczuk, Jemima Tsang, Jack Bickford-Smith, Robert H. Gilman, Kevin Tetteh, Chris Drakeley, C. Mark Smales, Michael A. Miles
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/165e40f9f490492697ff2f1922192a35
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spelling	oai:doaj.org-article:165e40f9f490492697ff2f1922192a352021-12-02T18:51:07ZGlycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera10.1038/s41598-020-73390-92045-2322https://doaj.org/article/165e40f9f490492697ff2f1922192a352020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73390-9https://doaj.org/toc/2045-2322Abstract Chagas disease is considered the most important parasitic disease in Latin America. The protozoan agent, Trypanosoma cruzi, comprises six genetic lineages, TcI-TcVI. Genotyping to link lineage(s) to severity of cardiomyopathy and gastrointestinal pathology is impeded by the sequestration and replication of T. cruzi in host tissues. We describe serology specific for TcI, the predominant lineage north of the Amazon, based on expression of recombinant trypomastigote small surface antigen (gTSSA-I) in the eukaryote Leishmania tarentolae, to allow realistic glycosylation and structure of the antigen. Sera from TcI-endemic regions recognised gTSSA-I (74/146; 50.7%), with no cross reaction with common components of gTSSA-II/V/VI recombinant antigen. Antigenicity was abolished by chemical (periodate) oxidation of gTSSA-I glycosylation but retained after heat-denaturation of conformation. Conversely, non-specific recognition of gTSSA-I by non-endemic malaria sera was abolished by heat-denaturation. TcI-specific serology facilitates investigation between lineage and diverse clinical presentations. Glycosylation cannot be ignored in the search for immunogenic antigens.Niamh MurphyBarrie RooneyTapan BhattacharyyaOmar Triana-ChavezAnja KruegerStuart M. HaslamVictoria O’RourkeMagdalena PańczukJemima TsangJack Bickford-SmithRobert H. GilmanKevin TettehChris DrakeleyC. Mark SmalesMichael A. MilesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Niamh Murphy Barrie Rooney Tapan Bhattacharyya Omar Triana-Chavez Anja Krueger Stuart M. Haslam Victoria O’Rourke Magdalena Pańczuk Jemima Tsang Jack Bickford-Smith Robert H. Gilman Kevin Tetteh Chris Drakeley C. Mark Smales Michael A. Miles Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera
description	Abstract Chagas disease is considered the most important parasitic disease in Latin America. The protozoan agent, Trypanosoma cruzi, comprises six genetic lineages, TcI-TcVI. Genotyping to link lineage(s) to severity of cardiomyopathy and gastrointestinal pathology is impeded by the sequestration and replication of T. cruzi in host tissues. We describe serology specific for TcI, the predominant lineage north of the Amazon, based on expression of recombinant trypomastigote small surface antigen (gTSSA-I) in the eukaryote Leishmania tarentolae, to allow realistic glycosylation and structure of the antigen. Sera from TcI-endemic regions recognised gTSSA-I (74/146; 50.7%), with no cross reaction with common components of gTSSA-II/V/VI recombinant antigen. Antigenicity was abolished by chemical (periodate) oxidation of gTSSA-I glycosylation but retained after heat-denaturation of conformation. Conversely, non-specific recognition of gTSSA-I by non-endemic malaria sera was abolished by heat-denaturation. TcI-specific serology facilitates investigation between lineage and diverse clinical presentations. Glycosylation cannot be ignored in the search for immunogenic antigens.
format	article
author	Niamh Murphy Barrie Rooney Tapan Bhattacharyya Omar Triana-Chavez Anja Krueger Stuart M. Haslam Victoria O’Rourke Magdalena Pańczuk Jemima Tsang Jack Bickford-Smith Robert H. Gilman Kevin Tetteh Chris Drakeley C. Mark Smales Michael A. Miles
author_facet	Niamh Murphy Barrie Rooney Tapan Bhattacharyya Omar Triana-Chavez Anja Krueger Stuart M. Haslam Victoria O’Rourke Magdalena Pańczuk Jemima Tsang Jack Bickford-Smith Robert H. Gilman Kevin Tetteh Chris Drakeley C. Mark Smales Michael A. Miles
author_sort	Niamh Murphy
title	Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera
title_short	Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera
title_full	Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera
title_fullStr	Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera
title_full_unstemmed	Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera
title_sort	glycosylation of trypanosoma cruzi tci antigen reveals recognition by chagasic sera
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/165e40f9f490492697ff2f1922192a35
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Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera

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