Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer

Abstract The intestinal epithelium plays a variety of roles including providing an effective physical barrier and innate immune protection against infection. Two-dimensional models of the intestinal epithelium, 2D enteroids, are a valuable resource to investigate intestinal cell biology and innate i...

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Autores principales:	Brigid Orr, Kate Sutton, Sonja Christian, Tessa Nash, Helle Niemann, Lone Lind Hansen, Mike J. McGrew, Stina Rikke Jensen, Lonneke Vervelde
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Chicken Intestinal epithelium 2D enteroid Organoid Barrier integrity Immune response Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/167002889dfb4348af093ac1e52e4d1d
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spelling	oai:doaj.org-article:167002889dfb4348af093ac1e52e4d1d2021-11-28T12:06:06ZNovel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer10.1186/s13567-021-01010-z1297-9716https://doaj.org/article/167002889dfb4348af093ac1e52e4d1d2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13567-021-01010-zhttps://doaj.org/toc/1297-9716Abstract The intestinal epithelium plays a variety of roles including providing an effective physical barrier and innate immune protection against infection. Two-dimensional models of the intestinal epithelium, 2D enteroids, are a valuable resource to investigate intestinal cell biology and innate immune functions and are suitable for high throughput studies of paracellular transport and epithelial integrity. We have developed a chicken 2D enteroid model that recapitulates all major differentiated cell lineages, including enterocytes, Paneth cells, Goblet cells, enteroendocrine cells and leukocytes, and self-organises into an epithelial and mesenchymal sub-layer. Functional studies demonstrated the 2D enteroids formed a tight cell layer with minimal paracellular flux and a robust epithelial integrity, which was maintained or rescued following damage. The 2D enteroids were also able to demonstrate appropriate innate immune responses following exposure to bacterial endotoxins, from Salmonella enterica serotype Typhimurium and Bacillus subtilis. Frozen 2D enteroids cells when thawed were comparable to freshly isolated cells. The chicken 2D enteroids provide a useful ex vivo model to study intestinal cell biology and innate immune function, and have potential uses in screening of nutritional supplements, pharmaceuticals, and bioactive compounds.Brigid OrrKate SuttonSonja ChristianTessa NashHelle NiemannLone Lind HansenMike J. McGrewStina Rikke JensenLonneke VerveldeBMCarticleChickenIntestinal epithelium2D enteroidOrganoidBarrier integrityImmune responseVeterinary medicineSF600-1100ENVeterinary Research, Vol 52, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chicken Intestinal epithelium 2D enteroid Organoid Barrier integrity Immune response Veterinary medicine SF600-1100
spellingShingle	Chicken Intestinal epithelium 2D enteroid Organoid Barrier integrity Immune response Veterinary medicine SF600-1100 Brigid Orr Kate Sutton Sonja Christian Tessa Nash Helle Niemann Lone Lind Hansen Mike J. McGrew Stina Rikke Jensen Lonneke Vervelde Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer
description	Abstract The intestinal epithelium plays a variety of roles including providing an effective physical barrier and innate immune protection against infection. Two-dimensional models of the intestinal epithelium, 2D enteroids, are a valuable resource to investigate intestinal cell biology and innate immune functions and are suitable for high throughput studies of paracellular transport and epithelial integrity. We have developed a chicken 2D enteroid model that recapitulates all major differentiated cell lineages, including enterocytes, Paneth cells, Goblet cells, enteroendocrine cells and leukocytes, and self-organises into an epithelial and mesenchymal sub-layer. Functional studies demonstrated the 2D enteroids formed a tight cell layer with minimal paracellular flux and a robust epithelial integrity, which was maintained or rescued following damage. The 2D enteroids were also able to demonstrate appropriate innate immune responses following exposure to bacterial endotoxins, from Salmonella enterica serotype Typhimurium and Bacillus subtilis. Frozen 2D enteroids cells when thawed were comparable to freshly isolated cells. The chicken 2D enteroids provide a useful ex vivo model to study intestinal cell biology and innate immune function, and have potential uses in screening of nutritional supplements, pharmaceuticals, and bioactive compounds.
format	article
author	Brigid Orr Kate Sutton Sonja Christian Tessa Nash Helle Niemann Lone Lind Hansen Mike J. McGrew Stina Rikke Jensen Lonneke Vervelde
author_facet	Brigid Orr Kate Sutton Sonja Christian Tessa Nash Helle Niemann Lone Lind Hansen Mike J. McGrew Stina Rikke Jensen Lonneke Vervelde
author_sort	Brigid Orr
title	Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer
title_short	Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer
title_full	Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer
title_fullStr	Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer
title_full_unstemmed	Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer
title_sort	novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer
publisher	BMC
publishDate	2021
url	https://doaj.org/article/167002889dfb4348af093ac1e52e4d1d
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Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer

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