Platelet-Derived Growth Factor Regulates the Biological Behavior of Oral Mucosal Fibroblasts by Inducing Cell Autophagy and Its Mechanism

Jie Wang,1,2 Lina Yang,2 Jialing You,2 Dada Wen,2 Bo Yang,2 Canhua Jiang1 1Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410078, People’s Republic of China; 2Department of Immunology, Xiangya School of Medicine, Central South University, Changsha...

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Autores principales:	Wang J, Yang L, You J, Wen D, Yang B, Jiang C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	pdgf-bb primary oral mucosal fibroblasts autophagy 3-ma biological behavior signaling pathway Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/16705b5bf5fd4869a37f7a24fd4b635d
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spelling	oai:doaj.org-article:16705b5bf5fd4869a37f7a24fd4b635d2021-12-02T16:17:55ZPlatelet-Derived Growth Factor Regulates the Biological Behavior of Oral Mucosal Fibroblasts by Inducing Cell Autophagy and Its Mechanism1178-7031https://doaj.org/article/16705b5bf5fd4869a37f7a24fd4b635d2021-07-01T00:00:00Zhttps://www.dovepress.com/platelet-derived-growth-factor-regulates-the-biological-behavior-of-or-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Jie Wang,1,2 Lina Yang,2 Jialing You,2 Dada Wen,2 Bo Yang,2 Canhua Jiang1 1Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410078, People’s Republic of China; 2Department of Immunology, Xiangya School of Medicine, Central South University, Changsha, 410078, People’s Republic of ChinaCorrespondence: Canhua JiangDepartment of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410078, Hunan, People’s Republic of ChinaTel/Fax +86-731-89753046Email wj0988@csu.edu.cnObjective: To explore the effect of platelet-derived growth factor (PDGF) on oral mucosal fibroblast autophagy and further elucidate the molecular mechanism by which PDGF-BB regulates the biological behavior of oral mucosal fibroblasts by inducing autophagy.Methods: Primary oral mucosal fibroblasts were isolated and cultured by the tissue block and trypsin methods and identified by indirect immunofluorescence vimentin detection. We detected the autophagy marker Beclin-1 and fibrosis marker Col-I of the primary oral mucosal fibroblasts at different time points after stimulating the fibroblasts with different PDGF-BB concentrations by Western blotting and determined the best experimental concentration and stimulation time of PDGF-BB. Then, indirect immunofluorescence, Western blotting, and quantitative real-time polymerase chain reaction (PCR) were used to detect the effect of PDGF-BB on the expression of autophagy-related and fibrotic proteins before and after 3-methyladenine (3-MA) intervention. Additionally, the effect of 3-MA on the proliferation and migration of primary oral mucosal fibroblasts stimulated by PDGF-BB was detected by the MTT method and a scratch experiment. The effect of PDGF-BB on Beclin-1 and phosphatidylinositol-3 kinase class 3 (PI3KC3) interaction was detected by co-immunoprecipitation.Results: The results demonstrated that PDGF-BB could induce autophagy of the oral mucosal fibroblasts, showing a certain time and dose correlation. It induced cell autophagy through Beclin-1 and PI3KC3 interaction to promote the proliferation, migration, conversion, and collagen synthesis of the fibroblasts. However, 3-MA inhibited the combination of Beclin-1 and PI3KC3 and weakened the fibroblasts’ proliferation, migration, conversion, and collagen synthesis activities.Conclusion: Overall, PDGF-BB induces autophagy through the Beclin-1 pathway to regulate the biological behavior of oral mucosal fibroblasts.Keywords: PDGF-BB, primary oral mucosal fibroblasts, autophagy, 3-MA, biological behavior, signaling pathwayWang JYang LYou JWen DYang BJiang CDove Medical Pressarticlepdgf-bbprimary oral mucosal fibroblastsautophagy3-mabiological behaviorsignaling pathwayPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 3405-3417 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pdgf-bb primary oral mucosal fibroblasts autophagy 3-ma biological behavior signaling pathway Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	pdgf-bb primary oral mucosal fibroblasts autophagy 3-ma biological behavior signaling pathway Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Wang J Yang L You J Wen D Yang B Jiang C Platelet-Derived Growth Factor Regulates the Biological Behavior of Oral Mucosal Fibroblasts by Inducing Cell Autophagy and Its Mechanism
description	Jie Wang,1,2 Lina Yang,2 Jialing You,2 Dada Wen,2 Bo Yang,2 Canhua Jiang1 1Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410078, People’s Republic of China; 2Department of Immunology, Xiangya School of Medicine, Central South University, Changsha, 410078, People’s Republic of ChinaCorrespondence: Canhua JiangDepartment of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410078, Hunan, People’s Republic of ChinaTel/Fax +86-731-89753046Email wj0988@csu.edu.cnObjective: To explore the effect of platelet-derived growth factor (PDGF) on oral mucosal fibroblast autophagy and further elucidate the molecular mechanism by which PDGF-BB regulates the biological behavior of oral mucosal fibroblasts by inducing autophagy.Methods: Primary oral mucosal fibroblasts were isolated and cultured by the tissue block and trypsin methods and identified by indirect immunofluorescence vimentin detection. We detected the autophagy marker Beclin-1 and fibrosis marker Col-I of the primary oral mucosal fibroblasts at different time points after stimulating the fibroblasts with different PDGF-BB concentrations by Western blotting and determined the best experimental concentration and stimulation time of PDGF-BB. Then, indirect immunofluorescence, Western blotting, and quantitative real-time polymerase chain reaction (PCR) were used to detect the effect of PDGF-BB on the expression of autophagy-related and fibrotic proteins before and after 3-methyladenine (3-MA) intervention. Additionally, the effect of 3-MA on the proliferation and migration of primary oral mucosal fibroblasts stimulated by PDGF-BB was detected by the MTT method and a scratch experiment. The effect of PDGF-BB on Beclin-1 and phosphatidylinositol-3 kinase class 3 (PI3KC3) interaction was detected by co-immunoprecipitation.Results: The results demonstrated that PDGF-BB could induce autophagy of the oral mucosal fibroblasts, showing a certain time and dose correlation. It induced cell autophagy through Beclin-1 and PI3KC3 interaction to promote the proliferation, migration, conversion, and collagen synthesis of the fibroblasts. However, 3-MA inhibited the combination of Beclin-1 and PI3KC3 and weakened the fibroblasts’ proliferation, migration, conversion, and collagen synthesis activities.Conclusion: Overall, PDGF-BB induces autophagy through the Beclin-1 pathway to regulate the biological behavior of oral mucosal fibroblasts.Keywords: PDGF-BB, primary oral mucosal fibroblasts, autophagy, 3-MA, biological behavior, signaling pathway
format	article
author	Wang J Yang L You J Wen D Yang B Jiang C
author_facet	Wang J Yang L You J Wen D Yang B Jiang C
author_sort	Wang J
title	Platelet-Derived Growth Factor Regulates the Biological Behavior of Oral Mucosal Fibroblasts by Inducing Cell Autophagy and Its Mechanism
title_short	Platelet-Derived Growth Factor Regulates the Biological Behavior of Oral Mucosal Fibroblasts by Inducing Cell Autophagy and Its Mechanism
title_full	Platelet-Derived Growth Factor Regulates the Biological Behavior of Oral Mucosal Fibroblasts by Inducing Cell Autophagy and Its Mechanism
title_fullStr	Platelet-Derived Growth Factor Regulates the Biological Behavior of Oral Mucosal Fibroblasts by Inducing Cell Autophagy and Its Mechanism
title_full_unstemmed	Platelet-Derived Growth Factor Regulates the Biological Behavior of Oral Mucosal Fibroblasts by Inducing Cell Autophagy and Its Mechanism
title_sort	platelet-derived growth factor regulates the biological behavior of oral mucosal fibroblasts by inducing cell autophagy and its mechanism
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/16705b5bf5fd4869a37f7a24fd4b635d
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Platelet-Derived Growth Factor Regulates the Biological Behavior of Oral Mucosal Fibroblasts by Inducing Cell Autophagy and Its Mechanism

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