Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes

Using phasor-FLIM (Fluorescence Lifetime Imaging Microscopy), Wang et al. reveal differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation. This study suggests the applicability of FLIM as a drug discovery tool as it reports perturbations in...

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Auteurs principaux:	Zhongying Wang, Tatyana Gurlo, Aleksey V. Matveyenko, David Elashoff, Peiyu Wang, Madeline Rosenberger, Jason A. Junge, Raymond C. Stevens, Kate L. White, Scott E. Fraser, Peter C. Butler
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/16706c9faa644994ba8974540e742fe0
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spelling	oai:doaj.org-article:16706c9faa644994ba8974540e742fe02021-12-02T15:45:31ZLive-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes10.1038/s42003-021-02113-12399-3642https://doaj.org/article/16706c9faa644994ba8974540e742fe02021-05-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02113-1https://doaj.org/toc/2399-3642Using phasor-FLIM (Fluorescence Lifetime Imaging Microscopy), Wang et al. reveal differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation. This study suggests the applicability of FLIM as a drug discovery tool as it reports perturbations in glucose metabolism in living islet cells.Zhongying WangTatyana GurloAleksey V. MatveyenkoDavid ElashoffPeiyu WangMadeline RosenbergerJason A. JungeRaymond C. StevensKate L. WhiteScott E. FraserPeter C. ButlerNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Zhongying Wang Tatyana Gurlo Aleksey V. Matveyenko David Elashoff Peiyu Wang Madeline Rosenberger Jason A. Junge Raymond C. Stevens Kate L. White Scott E. Fraser Peter C. Butler Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
description	Using phasor-FLIM (Fluorescence Lifetime Imaging Microscopy), Wang et al. reveal differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation. This study suggests the applicability of FLIM as a drug discovery tool as it reports perturbations in glucose metabolism in living islet cells.
format	article
author	Zhongying Wang Tatyana Gurlo Aleksey V. Matveyenko David Elashoff Peiyu Wang Madeline Rosenberger Jason A. Junge Raymond C. Stevens Kate L. White Scott E. Fraser Peter C. Butler
author_facet	Zhongying Wang Tatyana Gurlo Aleksey V. Matveyenko David Elashoff Peiyu Wang Madeline Rosenberger Jason A. Junge Raymond C. Stevens Kate L. White Scott E. Fraser Peter C. Butler
author_sort	Zhongying Wang
title	Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
title_short	Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
title_full	Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
title_fullStr	Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
title_full_unstemmed	Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
title_sort	live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/16706c9faa644994ba8974540e742fe0
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Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes

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