Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes

Using phasor-FLIM (Fluorescence Lifetime Imaging Microscopy), Wang et al. reveal differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation. This study suggests the applicability of FLIM as a drug discovery tool as it reports perturbations in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhongying Wang, Tatyana Gurlo, Aleksey V. Matveyenko, David Elashoff, Peiyu Wang, Madeline Rosenberger, Jason A. Junge, Raymond C. Stevens, Kate L. White, Scott E. Fraser, Peter C. Butler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/16706c9faa644994ba8974540e742fe0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:16706c9faa644994ba8974540e742fe0
record_format	dspace
spelling	oai:doaj.org-article:16706c9faa644994ba8974540e742fe02021-12-02T15:45:31ZLive-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes10.1038/s42003-021-02113-12399-3642https://doaj.org/article/16706c9faa644994ba8974540e742fe02021-05-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02113-1https://doaj.org/toc/2399-3642Using phasor-FLIM (Fluorescence Lifetime Imaging Microscopy), Wang et al. reveal differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation. This study suggests the applicability of FLIM as a drug discovery tool as it reports perturbations in glucose metabolism in living islet cells.Zhongying WangTatyana GurloAleksey V. MatveyenkoDavid ElashoffPeiyu WangMadeline RosenbergerJason A. JungeRaymond C. StevensKate L. WhiteScott E. FraserPeter C. ButlerNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Zhongying Wang Tatyana Gurlo Aleksey V. Matveyenko David Elashoff Peiyu Wang Madeline Rosenberger Jason A. Junge Raymond C. Stevens Kate L. White Scott E. Fraser Peter C. Butler Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
description	Using phasor-FLIM (Fluorescence Lifetime Imaging Microscopy), Wang et al. reveal differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation. This study suggests the applicability of FLIM as a drug discovery tool as it reports perturbations in glucose metabolism in living islet cells.
format	article
author	Zhongying Wang Tatyana Gurlo Aleksey V. Matveyenko David Elashoff Peiyu Wang Madeline Rosenberger Jason A. Junge Raymond C. Stevens Kate L. White Scott E. Fraser Peter C. Butler
author_facet	Zhongying Wang Tatyana Gurlo Aleksey V. Matveyenko David Elashoff Peiyu Wang Madeline Rosenberger Jason A. Junge Raymond C. Stevens Kate L. White Scott E. Fraser Peter C. Butler
author_sort	Zhongying Wang
title	Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
title_short	Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
title_full	Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
title_fullStr	Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
title_full_unstemmed	Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
title_sort	live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/16706c9faa644994ba8974540e742fe0
work_keys_str_mv	AT zhongyingwang livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes AT tatyanagurlo livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes AT alekseyvmatveyenko livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes AT davidelashoff livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes AT peiyuwang livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes AT madelinerosenberger livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes AT jasonajunge livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes AT raymondcstevens livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes AT katelwhite livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes AT scottefraser livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes AT petercbutler livecellimagingofglucoseinducedmetaboliccouplingofbandacellmetabolisminhealthandtype2diabetes
_version_	1718385734827638784

Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes

Ejemplares similares