Yap5 Competes With Hap4 for the Regulation of Iron Homeostasis Genes in the Human Pathogen Candida glabrata

The CCAAT-binding complex (CBC) is a conserved heterotrimeric transcription factor which, in fungi, requires additional regulatory subunits to act on transcription. In the pathogenic yeast Candida glabrata, CBC has a dual role. Together with the Hap4 regulatory subunit, it activates the expression o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Thierry Delaveau, Antonin Thiébaut, Médine Benchouaia, Jawad Merhej, Frédéric Devaux
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	yeast evolution stress response transcription factors regulation chromatine immunoprecipitation (ChIP) Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1687481ccd4143c4893eb67c2768739a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1687481ccd4143c4893eb67c2768739a
record_format	dspace
spelling	oai:doaj.org-article:1687481ccd4143c4893eb67c2768739a2021-12-01T07:22:09ZYap5 Competes With Hap4 for the Regulation of Iron Homeostasis Genes in the Human Pathogen Candida glabrata2235-298810.3389/fcimb.2021.731988https://doaj.org/article/1687481ccd4143c4893eb67c2768739a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.731988/fullhttps://doaj.org/toc/2235-2988The CCAAT-binding complex (CBC) is a conserved heterotrimeric transcription factor which, in fungi, requires additional regulatory subunits to act on transcription. In the pathogenic yeast Candida glabrata, CBC has a dual role. Together with the Hap4 regulatory subunit, it activates the expression of genes involved in respiration upon growth with non-fermentable carbon sources, while its association with the Yap5 regulatory subunit is required for the activation of iron tolerance genes in response to iron excess. In the present work, we investigated further the interplay between CBC, Hap4 and Yap5. We showed that Yap5 regulation requires a specific Yap Response Element in the promoter of its target gene GRX4 and that the presence of Yap5 considerably strengthens the binding of CBC to the promoters of iron tolerance genes. Chromatin immunoprecipitation (ChIP) and transcriptome experiments showed that Hap4 can also bind these promoters but has no impact on the expression of those genes when Yap5 is present. In the absence of Yap5 however, GRX4 is constitutively regulated by Hap4, similarly to the genes involved in respiration. Our results suggest that the distinction between the two types of CBC targets in C. glabrata is mainly due to the dependency of Yap5 for very specific DNA sequences and to the competition between Hap4 and Yap5 at the promoter of the iron tolerance genes.Thierry DelaveauAntonin ThiébautMédine BenchouaiaJawad MerhejFrédéric DevauxFrontiers Media S.A.articleyeastevolutionstress responsetranscription factorsregulationchromatine immunoprecipitation (ChIP)MicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	yeast evolution stress response transcription factors regulation chromatine immunoprecipitation (ChIP) Microbiology QR1-502
spellingShingle	yeast evolution stress response transcription factors regulation chromatine immunoprecipitation (ChIP) Microbiology QR1-502 Thierry Delaveau Antonin Thiébaut Médine Benchouaia Jawad Merhej Frédéric Devaux Yap5 Competes With Hap4 for the Regulation of Iron Homeostasis Genes in the Human Pathogen Candida glabrata
description	The CCAAT-binding complex (CBC) is a conserved heterotrimeric transcription factor which, in fungi, requires additional regulatory subunits to act on transcription. In the pathogenic yeast Candida glabrata, CBC has a dual role. Together with the Hap4 regulatory subunit, it activates the expression of genes involved in respiration upon growth with non-fermentable carbon sources, while its association with the Yap5 regulatory subunit is required for the activation of iron tolerance genes in response to iron excess. In the present work, we investigated further the interplay between CBC, Hap4 and Yap5. We showed that Yap5 regulation requires a specific Yap Response Element in the promoter of its target gene GRX4 and that the presence of Yap5 considerably strengthens the binding of CBC to the promoters of iron tolerance genes. Chromatin immunoprecipitation (ChIP) and transcriptome experiments showed that Hap4 can also bind these promoters but has no impact on the expression of those genes when Yap5 is present. In the absence of Yap5 however, GRX4 is constitutively regulated by Hap4, similarly to the genes involved in respiration. Our results suggest that the distinction between the two types of CBC targets in C. glabrata is mainly due to the dependency of Yap5 for very specific DNA sequences and to the competition between Hap4 and Yap5 at the promoter of the iron tolerance genes.
format	article
author	Thierry Delaveau Antonin Thiébaut Médine Benchouaia Jawad Merhej Frédéric Devaux
author_facet	Thierry Delaveau Antonin Thiébaut Médine Benchouaia Jawad Merhej Frédéric Devaux
author_sort	Thierry Delaveau
title	Yap5 Competes With Hap4 for the Regulation of Iron Homeostasis Genes in the Human Pathogen Candida glabrata
title_short	Yap5 Competes With Hap4 for the Regulation of Iron Homeostasis Genes in the Human Pathogen Candida glabrata
title_full	Yap5 Competes With Hap4 for the Regulation of Iron Homeostasis Genes in the Human Pathogen Candida glabrata
title_fullStr	Yap5 Competes With Hap4 for the Regulation of Iron Homeostasis Genes in the Human Pathogen Candida glabrata
title_full_unstemmed	Yap5 Competes With Hap4 for the Regulation of Iron Homeostasis Genes in the Human Pathogen Candida glabrata
title_sort	yap5 competes with hap4 for the regulation of iron homeostasis genes in the human pathogen candida glabrata
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1687481ccd4143c4893eb67c2768739a
work_keys_str_mv	AT thierrydelaveau yap5competeswithhap4fortheregulationofironhomeostasisgenesinthehumanpathogencandidaglabrata AT antoninthiebaut yap5competeswithhap4fortheregulationofironhomeostasisgenesinthehumanpathogencandidaglabrata AT medinebenchouaia yap5competeswithhap4fortheregulationofironhomeostasisgenesinthehumanpathogencandidaglabrata AT jawadmerhej yap5competeswithhap4fortheregulationofironhomeostasisgenesinthehumanpathogencandidaglabrata AT fredericdevaux yap5competeswithhap4fortheregulationofironhomeostasisgenesinthehumanpathogencandidaglabrata
_version_	1718405398656974848

Yap5 Competes With Hap4 for the Regulation of Iron Homeostasis Genes in the Human Pathogen Candida glabrata

Ejemplares similares