RNA-seq and Analysis of Argyrosomus japonicus Under Different Salinities

Salinity variation affects the physiological processes of fish. This study analyzed the transcriptome of the gill tissue of Argyrosomus japonicus to determine the significantly differentially expressed genes (DEGs) of A. japonicus under salinity changes. Transcriptome analysis of nine samples yielde...

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Autores principales:	Zhujun Li, Tianxiang Gao, Zhiqiang Han
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Argyrosomus japonicus salinity changes gill RNA-seq differentially expressed genes Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/1697f89e14b04aeebba0b3edfb8a772f
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id	oai:doaj.org-article:1697f89e14b04aeebba0b3edfb8a772f
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spelling	oai:doaj.org-article:1697f89e14b04aeebba0b3edfb8a772f2021-12-01T18:33:31ZRNA-seq and Analysis of Argyrosomus japonicus Under Different Salinities2296-774510.3389/fmars.2021.790065https://doaj.org/article/1697f89e14b04aeebba0b3edfb8a772f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.790065/fullhttps://doaj.org/toc/2296-7745Salinity variation affects the physiological processes of fish. This study analyzed the transcriptome of the gill tissue of Argyrosomus japonicus to determine the significantly differentially expressed genes (DEGs) of A. japonicus under salinity changes. Transcriptome analysis of nine samples yielded 55.873 Gb of clean data, 64,912 transcripts, and 29,567 unigenes, and 83.62% of the transcripts and 81.89% of the unigenes were annotated. Compared with the control group, the high- and low-salt groups showed 1,731 and 695 DEGs, respectively. Gene Ontology enrichment analysis revealed that the DEGs were significantly enriched in transportation, metabolism, and stress response. Kyoto Encyclopedia of Genes and Genomes pathway enrichment revealed that the DEGs were significantly enriched in some signaling pathways. Several key genes (KRT1, KRT2, ATP1A, LDH, PFN, ACTB_G1, TUBB, GZMB, MHC2, CCL19, EPX, ANXA5, ACBP, EHF, BHMT, COL1A, and RHOA) were related to salinity adaptation. When environmental salinity fluctuated, genes related to stress, immunity, ion transport, and metabolism became more sensitive. These results suggest that the adaptation of A. japonicus under salinity changes is a complex process that involves multiple genes acting together.Zhujun LiTianxiang GaoZhiqiang HanFrontiers Media S.A.articleArgyrosomus japonicussalinity changesgillRNA-seqdifferentially expressed genesScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Argyrosomus japonicus salinity changes gill RNA-seq differentially expressed genes Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	Argyrosomus japonicus salinity changes gill RNA-seq differentially expressed genes Science Q General. Including nature conservation, geographical distribution QH1-199.5 Zhujun Li Tianxiang Gao Zhiqiang Han RNA-seq and Analysis of Argyrosomus japonicus Under Different Salinities
description	Salinity variation affects the physiological processes of fish. This study analyzed the transcriptome of the gill tissue of Argyrosomus japonicus to determine the significantly differentially expressed genes (DEGs) of A. japonicus under salinity changes. Transcriptome analysis of nine samples yielded 55.873 Gb of clean data, 64,912 transcripts, and 29,567 unigenes, and 83.62% of the transcripts and 81.89% of the unigenes were annotated. Compared with the control group, the high- and low-salt groups showed 1,731 and 695 DEGs, respectively. Gene Ontology enrichment analysis revealed that the DEGs were significantly enriched in transportation, metabolism, and stress response. Kyoto Encyclopedia of Genes and Genomes pathway enrichment revealed that the DEGs were significantly enriched in some signaling pathways. Several key genes (KRT1, KRT2, ATP1A, LDH, PFN, ACTB_G1, TUBB, GZMB, MHC2, CCL19, EPX, ANXA5, ACBP, EHF, BHMT, COL1A, and RHOA) were related to salinity adaptation. When environmental salinity fluctuated, genes related to stress, immunity, ion transport, and metabolism became more sensitive. These results suggest that the adaptation of A. japonicus under salinity changes is a complex process that involves multiple genes acting together.
format	article
author	Zhujun Li Tianxiang Gao Zhiqiang Han
author_facet	Zhujun Li Tianxiang Gao Zhiqiang Han
author_sort	Zhujun Li
title	RNA-seq and Analysis of Argyrosomus japonicus Under Different Salinities
title_short	RNA-seq and Analysis of Argyrosomus japonicus Under Different Salinities
title_full	RNA-seq and Analysis of Argyrosomus japonicus Under Different Salinities
title_fullStr	RNA-seq and Analysis of Argyrosomus japonicus Under Different Salinities
title_full_unstemmed	RNA-seq and Analysis of Argyrosomus japonicus Under Different Salinities
title_sort	rna-seq and analysis of argyrosomus japonicus under different salinities
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1697f89e14b04aeebba0b3edfb8a772f
work_keys_str_mv	AT zhujunli rnaseqandanalysisofargyrosomusjaponicusunderdifferentsalinities AT tianxianggao rnaseqandanalysisofargyrosomusjaponicusunderdifferentsalinities AT zhiqianghan rnaseqandanalysisofargyrosomusjaponicusunderdifferentsalinities
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RNA-seq and Analysis of Argyrosomus japonicus Under Different Salinities

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