DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.

DNA damage is a threat to genomic integrity in all living organisms. Plants and green algae are particularly susceptible to DNA damage especially that caused by UV light, due to their light dependency for photosynthesis. For survival of a plant, and other eukaryotic cells, it is essential for an org...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Monika Hlavová, Mária Čížková, Milada Vítová, Kateřina Bišová, Vilém Zachleder
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16a171683ba44eaa9fc4354d058d1e97
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:16a171683ba44eaa9fc4354d058d1e97
record_format	dspace
spelling	oai:doaj.org-article:16a171683ba44eaa9fc4354d058d1e972021-11-18T06:53:57ZDNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.1932-620310.1371/journal.pone.0019626https://doaj.org/article/16a171683ba44eaa9fc4354d058d1e972011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21603605/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203DNA damage is a threat to genomic integrity in all living organisms. Plants and green algae are particularly susceptible to DNA damage especially that caused by UV light, due to their light dependency for photosynthesis. For survival of a plant, and other eukaryotic cells, it is essential for an organism to continuously check the integrity of its genetic material and, when damaged, to repair it immediately. Cells therefore utilize a DNA damage response pathway that is responsible for sensing, reacting to and repairing damaged DNA. We have studied the effect of 5-fluorodeoxyuridine, zeocin, caffeine and combinations of these on the cell cycle of the green alga Scenedesmus quadricauda. The cells delayed S phase and underwent a permanent G2 phase block if DNA metabolism was affected prior to S phase; the G2 phase block imposed by zeocin was partially abolished by caffeine. No cell cycle block was observed if the treatment with zeocin occurred in G2 phase and the cells divided normally. CDKA and CDKB kinases regulate mitosis in S. quadricauda; their kinase activities were inhibited by Wee1. CDKA, CDKB protein levels were stabilized in the presence of zeocin. In contrast, the protein level of Wee1 was unaffected by DNA perturbing treatments. Wee1 therefore does not appear to be involved in the DNA damage response in S. quadricauda. Our results imply a specific reaction to DNA damage in S. quadricauda, with no cell cycle arrest, after experiencing DNA damage during G2 phase.Monika HlavováMária ČížkováMilada VítováKateřina BišováVilém ZachlederPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19626 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Monika Hlavová Mária Čížková Milada Vítová Kateřina Bišová Vilém Zachleder DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.
description	DNA damage is a threat to genomic integrity in all living organisms. Plants and green algae are particularly susceptible to DNA damage especially that caused by UV light, due to their light dependency for photosynthesis. For survival of a plant, and other eukaryotic cells, it is essential for an organism to continuously check the integrity of its genetic material and, when damaged, to repair it immediately. Cells therefore utilize a DNA damage response pathway that is responsible for sensing, reacting to and repairing damaged DNA. We have studied the effect of 5-fluorodeoxyuridine, zeocin, caffeine and combinations of these on the cell cycle of the green alga Scenedesmus quadricauda. The cells delayed S phase and underwent a permanent G2 phase block if DNA metabolism was affected prior to S phase; the G2 phase block imposed by zeocin was partially abolished by caffeine. No cell cycle block was observed if the treatment with zeocin occurred in G2 phase and the cells divided normally. CDKA and CDKB kinases regulate mitosis in S. quadricauda; their kinase activities were inhibited by Wee1. CDKA, CDKB protein levels were stabilized in the presence of zeocin. In contrast, the protein level of Wee1 was unaffected by DNA perturbing treatments. Wee1 therefore does not appear to be involved in the DNA damage response in S. quadricauda. Our results imply a specific reaction to DNA damage in S. quadricauda, with no cell cycle arrest, after experiencing DNA damage during G2 phase.
format	article
author	Monika Hlavová Mária Čížková Milada Vítová Kateřina Bišová Vilém Zachleder
author_facet	Monika Hlavová Mária Čížková Milada Vítová Kateřina Bišová Vilém Zachleder
author_sort	Monika Hlavová
title	DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.
title_short	DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.
title_full	DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.
title_fullStr	DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.
title_full_unstemmed	DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.
title_sort	dna damage during g2 phase does not affect cell cycle progression of the green alga scenedesmus quadricauda.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/16a171683ba44eaa9fc4354d058d1e97
work_keys_str_mv	AT monikahlavova dnadamageduringg2phasedoesnotaffectcellcycleprogressionofthegreenalgascenedesmusquadricauda AT mariacizkova dnadamageduringg2phasedoesnotaffectcellcycleprogressionofthegreenalgascenedesmusquadricauda AT miladavitova dnadamageduringg2phasedoesnotaffectcellcycleprogressionofthegreenalgascenedesmusquadricauda AT katerinabisova dnadamageduringg2phasedoesnotaffectcellcycleprogressionofthegreenalgascenedesmusquadricauda AT vilemzachleder dnadamageduringg2phasedoesnotaffectcellcycleprogressionofthegreenalgascenedesmusquadricauda
_version_	1718424214113878016

DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.

Ejemplares similares